LOCUS       sequence-369066-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  optimisation of phosphinothricin (Basta) selection in plant cells; 
            combines kanamycin resistance gene (nptII) and Basta resistance gene
            (bar).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Kobercova E, Srba M, Fischer L
  TITLE     Sulfadiazine and phosphinothricin selection systems optimised for 
            the transformation of tobacco BY-2 cells.
  JOURNAL   Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7.
  PUBMED    36609768
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7."
COMMENT     Sequence Label: pDGB3omega1_KanR_BastaR
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..280)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(320..1114)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(431..450)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1041..1060
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1148..1327)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1832..2176
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2120..2140
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2328..2876
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(3446..3463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3446..3462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3455..3477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3521..3545
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4846..5475
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5904..6977
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7043..7237
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7473..7495)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7581..7721
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7636..7655
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7736..7753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7907..8495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7987..8006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8583..9374)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10160..10184
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggagagcgat cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata
       61 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
      121 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
      181 catgcttaac gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat
      241 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt
      301 ccgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct
      361 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      421 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      481 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      541 aggcgtcgcc gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
      601 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      661 gaccggcttc catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg
      721 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      781 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      841 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      901 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
      961 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     1021 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     1081 gagaacctgc gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat
     1141 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     1201 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     1261 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt
     1321 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcatac
     1381 cgtgactccc ttaattctca tgtatctccg tcaggagatg cctgcaggtc aacatggtgg
     1441 agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg
     1501 caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
     1561 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
     1621 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
     1681 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     1741 aagtggattg atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca
     1801 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     1861 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     1921 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1981 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     2041 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     2101 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     2161 ttcatttgga gaggacctcg agaattaatt ctcaacacaa catatacaaa acaaacgaat
     2221 ctcaagcaat caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa
     2281 aagcaatttt ctgaaaattt tcaccattta cgaacggggg atctaccatg agcccagaac
     2341 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     2401 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     2461 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     2521 acggcgaggt cgccggcata gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     2581 ggacggccga gtcgaccgtg tacgtatccc cccgccacca gcggacggga ctgggctcca
     2641 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     2701 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     2761 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
     2821 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatctgct
     2881 caacaatcta gctagagttt gctcctatct atatgtaata aggtatgctg atatgcacta
     2941 ttcaaatagg agcattagct atgtttgtta atgtcacttt atgttatgtg ggtaagtcac
     3001 ctaagacact ccacgtacct acttgttgtc tcttacgcgg ctttaataaa tcttctgccc
     3061 ttgttccata tttactaatt atccctttct tcactaaaag aaaattgtta tcattaagta
     3121 ttagtcttta gaacatatga ggtctttaat tgggtaggtt ttacaaatta actaatataa
     3181 aatgtcataa aatccacgtg gttaaacaaa tgcagaaaat cgacgtcgtc tattggaccg
     3241 acagttgcta ttaatataat gggccaccat agtagactga caaataaatt acctgacaac
     3301 atcgtttcac aaaaaaacaa acacaaaaag ggagtgcatt ttccagggca tttttgtaat
     3361 aaaaaacagt taaaagggag tgcaatagaa atataggggt gtggaaatag tgatttgagc
     3421 acgtcttgaa gcgaattagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3481 accctggcgt tacccaactc gctgtcatga gaccggatcc tgacaggata tattggcggg
     3541 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     3601 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa
     3661 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     3721 cgtcatctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     3781 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     3841 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     3901 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     3961 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     4021 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     4081 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     4141 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     4201 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     4261 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     4321 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     4381 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     4441 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     4501 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     4561 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     4621 gtacgtgttc gagccgcccg cgcacctctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4681 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4741 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4801 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4861 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4921 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4981 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     5041 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5101 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5161 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5221 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5281 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5341 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5401 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5461 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5521 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5581 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5641 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5701 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5761 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5821 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
     5881 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     5941 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     6001 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6061 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     6121 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6181 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6241 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6301 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6361 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6421 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6481 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6541 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6601 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6661 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6721 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6781 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6841 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6901 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6961 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     7021 aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7081 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7141 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7201 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7261 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7321 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     7381 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     7441 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggtgacg
     7501 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7561 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     7621 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7681 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     7741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8101 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8341 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     8581 gattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     8641 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     8701 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     8761 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     8821 tttcgctcat caccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     8881 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     8941 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     9001 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     9061 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     9121 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     9181 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     9241 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     9301 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     9361 accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     9421 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     9481 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     9541 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     9601 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     9661 ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc atttctgtcc
     9721 tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     9781 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     9841 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     9901 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     9961 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
    10021 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
    10081 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
    10141 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataacg gatccggtct
    10201 ca
//
