LOCUS       sequence-369067-        4401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transcriptional unit for sulfadiazine resistance; plant expression 
            driven by the Pnos promoter; suitable for quintuple assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Kobercova E, Srba M, Fischer L
  TITLE     Sulfadiazine and phosphinothricin selection systems optimised for 
            the transformation of tobacco BY-2 cells.
  JOURNAL   Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7.
  PUBMED    36609768
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7."
COMMENT     Sequence Label: pDGB1alpha11_SulR
FEATURES             Location/Qualifiers
     source          1..4401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..94
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      374..809
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    936..958
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        1054..1233
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      2302..2554
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2589..2607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2795..2819
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2910..3498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2990..3009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3669..4401,1..83))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(3669..4401,1..83))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     join(4392..4401,1..10)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac
       61 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
      121 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
      181 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat
      241 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc
      301 acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg
      361 attcaggcga tccccatcca acagcccgcc gtcgagcggg cttttttatc cccggaagcc
      421 tgtggataga gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc cttgattcac
      481 ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag ggtacgtgaa
      541 atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct aatcaaaaag
      601 gcacgtgaga acgctaatag ccctttcaga tcaacagctt gcaaacaccc ctcgctccgg
      661 caagtagtta cagcaagtag tatgttcaat tagcttttca attatgaata tatatatcaa
      721 ttattggtcg cccttggctt gtggacaatg cgctacgcgc accggctccg cccgtggaca
      781 accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca acccggcggc cggccgcaac
      841 agatcgtttt ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg gcaacctctc
      901 gggcttctgg atttccgatc cccggaatta gatcttggca ggatatattg tggtgtaaag
      961 aattcgtctc aggagataca tgagaattaa gggagtcacg gtatgacccc cgccgatgac
     1021 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga
     1081 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
     1141 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact
     1201 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgat
     1261 cgaggcaatg gcatctatga tttcttcttc agctgtgact acagtcagcc gtgcttctac
     1321 ggtgcaatcg gccgctgtgg ctccattcgg cggcctcaaa tccatgactg gattcccagt
     1381 taagaaggtc aacactgaca ttacttccat tacaagcaat ggtggaagag taaagtgcat
     1441 ggtgacggtg ttcggcattc tgaatctcac cgaggactcc ttcttcgatg agagccggcg
     1501 gctagacccc gccggcgctg tcacggctgc gatcgaaatg ctgcgagtcg gatcagacgt
     1561 cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg ccagtatcgc cggccgatga
     1621 gatcagacgt attgcgccgc tcttagacgc cctgtccgat cagatgcacc gtgtttcaat
     1681 cgacagcttc caaccggaaa cccagcgcta tgcgctcaag cgcggcgtgg gctacctgaa
     1741 cgacatccaa ggatttcctg accctgcgct ctatcccgat attgctgagg cggactgtag
     1801 gctggtggtt atgcactcag cgcagcggga tggcatcgcc acccgcaccg gtcaccttcg
     1861 acccgaagac gcgctcgacg agattgtgcg gttcttcgag gcgcgggttt ccgccttgcg
     1921 acggagcggg gtcgctgccg accggctcat cctcgatccg gggatgggat ttttcttgag
     1981 ccccgcaccg gaaacatcgc tgcacgtgct gtcgaacctt caaaagctga agtcggcgtt
     2041 ggggcttccg ctattggtat cggtgtcgcg gaaatccttc ttgggcgcca ccgttggcct
     2101 tcctgtaaag gatctgggtc cagcgagcct tgcggcggaa cttcacgcga tcggcaatgg
     2161 cgctgactac gtccgcaccc acgcgcctgg agatctgcga agcgcaatca ccatctcgga
     2221 aaccctcgcg aaatttcgca gtcgcgacgc cagagatcga gggttagatc atgcctaagc
     2281 ttggaatgga tcttcgatcc cgatcgttca aacatttggc aataaagttt cttaagattg
     2341 aatcctgttg ccggtcttgc gacgattatc atataatttc tgttgaatta cgttaagcat
     2401 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     2461 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     2521 ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tgccaagcta attcttgaag
     2581 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     2641 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2761 atgggaccga ctcgcgctcc attgagacga attctgacag gatatattgg cgggtaaact
     2821 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     2881 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2941 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3001 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3061 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3121 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3181 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3241 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3301 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3361 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     3421 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3481 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3541 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3601 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     3661 tctagtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     3721 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     3781 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     3841 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     3901 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     3961 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     4021 cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     4081 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     4141 tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac
     4201 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     4261 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     4321 ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat
     4381 tgcccgacat tatcgcgagc c
//
