LOCUS       sequence-369077-        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of genetically encoded protease indicator 
            SPW-RHH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Gohil K, Wu SY, Takahashi-Yamashiro K, Shen Y, Campbell RE
  TITLE     Biosensor Optimization Using a Forster Resonance Energy Transfer 
            Pair Based on mScarlet Red Fluorescent Protein and an 
            mScarlet-Derived Green Fluorescent Protein.
  JOURNAL   ACS Sens. 2023 Feb 24;8(2):587-597. doi: 10.1021/acssensors.2c01730.
            Epub 2023 Jan 24.
  PUBMED    36693235
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01730"; journalName: "ACS Sens"; date: 
            "2023-02-24- 24"; volume: "8"; issue: "2"; pages: "587-597"
COMMENT     Sequence Label: pBAD-SPW-RHH
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             727..738
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtacccca gggacggcgt gctgaagggc
      481 gacattcata tggccctgcg cctgaaggac ggcggccact acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcgg taccggaggg agtgggggaa caggcgggag tggaggaaca
      721 gggggatggg aattgcaaag cgggggcact ggcgggtcgg gtggtactgg tggaagtggt
      781 gcatgcggcg aggcattgat caaggagtac atgcggttca aggtgcacat ggagggctcc
      841 atggacggcc acgagttcga gatcgagggc gagggcgagg gccgcccnta cgagggcacc
      901 cataccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg
      961 tcccctcagt tcgggtacgg ctccagggcc ttcatcaagc accccgccga catccccgac
     1021 tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1081 ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct gatccacaag
     1141 gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagag gaagacaatg
     1201 ggctgggaag cgtccaccga gcggttgtac cccagggacg gcgtgctgaa gggcgacatt
     1261 catatggccc tgcgcctgaa ggacggcggc cactacctgg cggactgcaa gaccacctac
     1321 aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc
     1381 acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctcaga gggccgccac
     1441 tccaccggcg gcatggacga gctgtacaag
//
