LOCUS       sequence-369083-        1956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of genetically encoded calcium indicator SCcpV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Gohil K, Wu SY, Takahashi-Yamashiro K, Shen Y, Campbell RE
  TITLE     Biosensor Optimization Using a Forster Resonance Energy Transfer 
            Pair Based on mScarlet Red Fluorescent Protein and an 
            mScarlet-Derived Green Fluorescent Protein.
  JOURNAL   ACS Sens. 2023 Feb 24;8(2):587-597. doi: 10.1021/acssensors.2c01730.
            Epub 2023 Jan 24.
  PUBMED    36693235
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.2c01730"; journalName: "ACS Sens"; date: 
            "2023-02-24- 24"; volume: "8"; issue: "2"; pages: "587-597"
COMMENT     Sequence Label: pCDNA-SCcpV
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1159..1215
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 atggttagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcgc atgcctgccg gaccaactga ctgaagagca gatcgcagaa
      721 tttaaagagg ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag
      781 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      841 atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt cctgacaatg
      901 atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc gttcggtgtg
      961 tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac
     1021 cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat
     1081 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gggtggcgga
     1141 ggttctgtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt cagagctata
     1201 ggtcggctga gctcactaga gggggtccag cttgcagatc actaccagca gaatactccg
     1261 atcggggatg ggccggttct cctgcctgac aaccactacc tgtcatacca gtccaagctg
     1321 tccaaagatc ctaatgaaaa gcgggatcac atggtcctgc tggaatttgt caccgccgcc
     1381 ggaatcactc tcggaatgga cgagctgtac aaaggtggga ccgggggatc tatggtgagc
     1441 aagggcgaag aattgttcac tggtgtagtc cctattttgg tggaactgga tggggatgtc
     1501 aacggtcata aattcagcgt gcgaggggag ggtgaggggg acgcaactaa tggcaaattg
     1561 acactcaagc tcatctgcac aacggggaaa ctgcccgtgc cgtggcccac actcgttact
     1621 actctaggat atggccttat gtgtttcgcc cgctacccag accacatgaa acaacacgat
     1681 ttctttaaat cagccatgcc agaggggtac gtccaggaga gaactatctt cttcaaggac
     1741 gatggtaatt acaagactag ggccgaggtg aaattcgaag gggatacact tgtcaaccga
     1801 atcgagctga agggaatcga ctttaaggag gacggaaaca tactcgggca taagttggaa
     1861 tacaactaca acagtcacaa tgtttacatc acagccgata agcaaaagaa cggcattaag
     1921 gctaatttta agatccgtca caacattgag gatggt
//
