LOCUS       sequence-36909-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kitazono AA
  TITLE     Optimized protocols and plasmids for in vivo cloning in yeast.
  JOURNAL   Gene. 2011 Sep 15;484(1-2):86-9. doi: 10.1016/j.gene.2011.06.006. 
            Epub 2011 Jun 22.
  PUBMED    21722715
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-09-15"; volume: "484(1-2):86-9. doi"; pages: " 
            10.1016/j.gene.2011.06.006. Epub 2011 Jun 22"
COMMENT     Sequence Label: pRS415-IVb
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..72
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     56..72
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(175..191)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(216..235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(217..235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(245..262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(245..261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(254..276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      402..858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(489..508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     699..720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agggattanc ctactaaagg gacaaaagct ggagctccac cgcggtggcg gccgctctag
       61 aactagtgga tcccccgggc tgcagatctc aagcgctacg tagtttaaac atatgcatgc
      121 tagccggcca tggacgtctc gcgaaggcct gcaggaattc gatatcaagc ttatcgatac
      181 cgtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc
      241 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      361 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
      421 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      481 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      541 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      601 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      661 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      721 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      781 ggcctattgg ttaaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      841 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      901 acaccgcata tcgaccggtc gaggaaanct tctagtatat ctacatacct
//
