LOCUS       sequence-36911-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kitazono AA
  TITLE     Optimized protocols and plasmids for in vivo cloning in yeast.
  JOURNAL   Gene. 2011 Sep 15;484(1-2):86-9. doi: 10.1016/j.gene.2011.06.006. 
            Epub 2011 Jun 22.
  PUBMED    21722715
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-09-15"; volume: "484(1-2):86-9. doi"; pages: " 
            10.1016/j.gene.2011.06.006. Epub 2011 Jun 22"
COMMENT     Sequence Label: pRS415-IVa
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..71
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     55..71
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(174..190)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(175..191)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(215..234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(216..234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(244..261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(244..260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(253..275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      401..858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(488..507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     698..719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 anggantanc ctactaaggg acaaaagctg gagctccacc gcggtggcgg ccgctctaga
       61 actagtggat cccccgggct gcaggccttc gcgagacgtc catggccggc tagcatgcat
      121 atgtttaaac tacgtagcgc ttgagatctg caggaattcg atatcaagct tatcgatacc
      181 gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
      241 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
      421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      781 gcctattggt taaaaaatga gctgatttaa caaaaaattt taacgcgaat tttaacaaaa
      841 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      901 acaccgcata tcgaccggtt cgagganaan tttctagtan atctacatac
//
