LOCUS       sequence-36913-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kitazono AA
  TITLE     Optimized protocols and plasmids for in vivo cloning in yeast.
  JOURNAL   Gene. 2011 Sep 15;484(1-2):86-9. doi: 10.1016/j.gene.2011.06.006. 
            Epub 2011 Jun 22.
  PUBMED    21722715
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-09-15"; volume: "484(1-2):86-9. doi"; pages: " 
            10.1016/j.gene.2011.06.006. Epub 2011 Jun 22"
COMMENT     Sequence Label: pRS413-IVb
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(173..189)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(214..233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(215..233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(243..260)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(243..259)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(252..274)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      400..858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(487..506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     697..718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaggattacc tactaaggga caaaagctgg agctccaccg cggtggcggc cgctctagaa
       61 ctagtggatc ccccgggctg cagatctcaa gcgctacgta gtttaaacat atgcatgcta
      121 gccggccatg gacgtctcgc gaaggcctgc aggaattcga tatcaagctt atcgataccg
      181 tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
      241 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      301 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      361 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
      421 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      481 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      541 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      601 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      661 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      721 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      781 cctattggtt taaaaaaatg agctgattta acaaaaattt aacgcgaatt tttaacaaaa
      841 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      901 acaccgca
//
