LOCUS       sequence-369133-       10376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ChR2-3xM channelrhodopsin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Hayward RF, Brooks FP 3rd, Yang S, Gao S, Cohen AE
  TITLE     Diminishing neuronal acidification by channelrhodopsins with low 
            proton conduction.
  JOURNAL   eLife. 2023 Oct 6;12:RP86833. doi: 10.7554/eLife.86833.
  PUBMED    37801078
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-10-6"; volume: "12:RP86833. doi"; pages: " 
            10.7554/eLife.86833"
COMMENT     Sequence Label: pLenti_CMV_ChR2-3M-YFP
FEATURES             Location/Qualifiers
     source          1..10376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..102
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             join(1099..1101,1102..1104,1105..1815)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1144..1165)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1405..1424)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1752..1773
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1868..2456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1921..1941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2339..2350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2459..2475)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2460..2476)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2981..3161
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3187..3204)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3193..3417
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3463..3891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3550..3569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3760..3781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3900..3920)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3905..4234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4085..4220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4282..4329
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4348..4722
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4852..4973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4889..4908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4943..4962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5022..5038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5022..5038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5035..5057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5046..5062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5070..5076,5077..5094,5095..5100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5115..5136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5253..5270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5424..6012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5504..5523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6183..6974,6975..7043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6806..6825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7044..7148)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7223..7242)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7414..7793
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7795..7993
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8011..8191
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8238..8363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8856..9089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9274..9318
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9616..9733
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9792..10095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10096..10299
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10296..10320
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgcgacccc aaatactcct cttgctggct ttgttgaccc ttggactggc taacggaaca
       61 gaaggtccca acttctacgt tcctttcagc aataagacag gcgtagtcag atccggtagt
      121 cgcgagctgc tatttgtaac gaacccagta gtcgtcaatg gctctgtact tgtgcctgag
      181 gaccagtgtt actgcgcggg ctggattgag tcgcgtggca caaacggtgc ccaaacggcg
      241 tcgaacgtgc tgcaatggct tgctgctggc ttctccatcc tactgcttat gttttacgcc
      301 taccaaacat ggaagtcaac ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg
      361 gtcaaggtga ttctcgagtt cttcttcaac tttaagaacc cgtccatgct gtatctagcc
      421 acaggccacc gcgtccagtg gttgcgttac gccgagtggc ttctcacctg cccggtcatt
      481 ctcattcagc tgtcaaacct gacgggcttg tccaacgact acagcaggcg caccatgggt
      541 ctgcttgtgt ctcatattgg cacaattgtg tggggcgcca cttccgccat ggccaccgga
      601 tacgtcaagg tcatcttctt ctgcctgggt ctgtgttatg gtgctaacac gttctttcac
      661 gctgccaagg cctacatcga gggttaccac accgtgccga agggccggtg tcgccaggtg
      721 gtgactggca tggcttggct cttcttcgta tcatggggta tgttccccat cctgttcatc
      781 ctcggccccg agggcttcgg cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc
      841 attgacctga tgtcgaagaa ctgctggggt ctgctcggcc actacctgcg cgtgctgatc
      901 cacgagcata tcctcatcca cggcgacatt cgcaagacca ccaaattgaa cattggtggc
      961 actgagattg aggtcgagac gctggtggag gacgaggccg aggctggcgc ggtagtcgag
     1021 aaatccagaa ttacttctga aggggagtat atccctctgg atcaaataga catcaatgtt
     1081 gtcgacacta gttctagaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1141 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1201 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1261 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     1321 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1381 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1441 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1501 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1561 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1621 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1681 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     1741 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1801 gacgagctgt acaagtctag attttgctat gaaaatgaag tttaaattcg atatcaagct
     1861 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2461 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     2521 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     2581 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     2641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     2701 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     2761 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     2821 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     2881 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     2941 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     3001 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     3061 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     3121 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     3181 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3241 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3301 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3361 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3421 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3901 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3961 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4141 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4201 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4261 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     4321 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     4381 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     4441 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     4501 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     4561 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     4621 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     4681 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     4741 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4801 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4981 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5041 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5101 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5161 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5221 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5281 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5341 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5401 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5461 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5521 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5581 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5641 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5701 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5761 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5821 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5881 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5941 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6001 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6061 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6121 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6181 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6241 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6301 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6361 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6421 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6481 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6541 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6601 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6661 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6721 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6781 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6841 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6901 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6961 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7021 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7081 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7141 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     7201 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     7261 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7321 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7381 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7441 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7501 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7561 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7621 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7681 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7741 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7801 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7861 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7921 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7981 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8041 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8101 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8161 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8221 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8281 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8341 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8401 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8461 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8521 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8581 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8641 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8701 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8761 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8821 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8881 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8941 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9001 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9061 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9121 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9181 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9241 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9301 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9361 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9421 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9481 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     9541 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     9601 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9661 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9721 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaagct
     9781 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9841 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9901 cattgacgtc aatgggtgga gcatttacgg taaactgccc acttggcagt acatcaagtg
     9961 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    10021 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    10081 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    10141 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    10201 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    10261 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
    10321 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagag
//
