LOCUS       sequence-369135-       10386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PsCatCh2.0 channelrhodopsin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Hayward RF, Brooks FP 3rd, Yang S, Gao S, Cohen AE
  TITLE     Diminishing neuronal acidification by channelrhodopsins with low 
            proton conduction.
  JOURNAL   eLife. 2023 Oct 6;12:RP86833. doi: 10.7554/eLife.86833.
  PUBMED    37801078
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2023-10-6"; volume: "12:RP86833. doi"; pages: " 
            10.7554/eLife.86833"
COMMENT     Sequence Label: pLenti_CMV_PsCatCh2.0-YFP
FEATURES             Location/Qualifiers
     source          1..10386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..102
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             join(1108..1110,1111..1113,1114..1824)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1153..1174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1414..1433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1761..1782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1878..2466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1931..1951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2349..2360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2469..2485)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2470..2486)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2991..3171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3197..3214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3203..3427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3473..3901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3560..3579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3770..3791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3910..3930)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3915..4244
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4095..4230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4157..4176
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4292..4339
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4358..4732
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4862..4983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4899..4918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4953..4972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5032..5048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5032..5048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5045..5067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5056..5072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5080..5086,5087..5104,5105..5110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5125..5146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5263..5280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5434..6022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5514..5533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6193..6984,6985..7053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6816..6835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7054..7158)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7233..7252)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7424..7803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7805..8003
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8021..8201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8248..8373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8866..9099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9284..9328
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9626..9743
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9802..10105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10106..10309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10306..10330
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atgcgacccc aaatactcct cttgctggct ttgttgaccc ttggactggc taacggaaca
       61 gaaggtccca acttctacgt tcctttcagc aataagacag gcgtagtcag atccatgggc
      121 ttccagctca atccggaata cctgaacgaa acaattttgc tggacgactg tacgccaatc
      181 tatctgaacg tcggtcccct gtgggagcaa aaggttgccc gaggtacgca atggtttggt
      241 gttatcctct cgttggcgtt tctcatctat tatatatgga taacatataa ggcgacttgc
      301 ggatgggaag aactgtacgt gtgtacgatc gaattttgta aaattgtaat tgaactctac
      361 ttcgagtttt cgcctccggc gatgatatat cagaccaacg gagaggtaac gccctggttg
      421 cgatatgctg aatggctctt gacctgccca gtcatttgca tacatttgag taacattaca
      481 ggactcaatg acgactattc gggccgaact atgtccctga tcacatccga cctgggaggc
      541 atctgcatgg ccgttacttc cgccttgagt aaaggctggt tgaagtggct ctttttcgtg
      601 atcggctgtt gttacggtgc tagtacgttt tatcatgcgg cgctcatata tatagaaagt
      661 tactacacta tgcctcacgg tgtttgtaaa aacatggtat tggcgatggc agcggtattt
      721 tttacaagtt ggttcatgtt tcccggtttg ttcctggcag gccctgaagg aaccaatgca
      781 ctgtcgtggg cgggctcgac tatcggtcat accgtggcag atttgctctc caaaaacgcc
      841 tggggtatga ttggtcactt cctccgactg gagatacaca aacatataat tattcatggt
      901 gatgttcgtc gcccgataac ggttaatacc ttgggacgag aagtcacggt gtcgtgtttt
      961 gtcgataaag aagaggagga tgaagacgaa cgtatcagca ctaagaccta cgcgaaccgg
     1021 gcactcgaga aatccagaat tacttctgaa ggggagtata tccctctgga tcaaatagac
     1081 atcaatgttg tcgacactag ttctagaatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     1321 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1561 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1801 ctcggcatgg acgagctgta caagtctaga ttttgctatg aaaatgaagt ttaaaattcg
     1861 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     1921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     1981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2281 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2461 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     2521 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     2581 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     2641 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     2701 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     2761 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     2821 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     2881 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     2941 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     3001 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3061 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3121 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     3181 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3241 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3301 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3361 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3421 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3901 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3961 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4201 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4261 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     4321 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     4381 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     4441 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     4501 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     4561 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     4621 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     4681 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     4741 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4801 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4861 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4981 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5041 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5101 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7201 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7261 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     7321 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     7381 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     7441 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7501 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7561 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7621 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7681 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7741 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7801 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     7861 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     7921 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     7981 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
     8041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8161 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8221 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8281 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8341 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8401 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8461 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8521 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8581 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8641 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8701 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8761 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8821 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8881 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8941 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9001 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9061 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9121 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9181 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9241 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9301 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9361 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9421 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9481 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9541 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9601 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9661 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9721 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     9781 taattaagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     9841 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     9901 agggactttc cattgacgtc aatgggtgga gcatttacgg taaactgccc acttggcagt
     9961 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
    10021 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
    10081 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
    10141 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
    10201 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
    10261 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
    10321 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact
    10381 ctagag
//
