LOCUS       sequence-369137-         827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes enhanced green fluorescent protein linked to P(11)4 
            self-assembling peptide via a GS-linker sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Shanbhag BK, Liu C, Pradeep GC, Younas T, Hu KKY, Fulcher AJ, Struwe
            WB, Steer D, Dumsday G, Harper IS, Kukura P, Haritos VS, He L
  TITLE     Custom Design of Protein Particles as Multifunctional Biomaterials
  JOURNAL   Adv. Funct. Mater. 2022, 32, 2108039
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv. Funct.
            Mater. 2022, 32, 2108039"
COMMENT     Sequence Label: pET28a-eGFP-P(11)4
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccatgggcca tcaccatcac catcacaccg atccgatggt ctctaaaggt gaagaactgt
       61 ttacgggcgt ggttccgatt ctggttgaac tggatggtga cgtcaatggc cataaattca
      121 gcgtttctgg cgaaggtgaa ggcgacgcaa cctatggtaa actgacgctg aaatttatct
      181 gcaccaccgg taaactgccg gtgccgtggc cgaccctggt taccaccctg gcgtatggcg
      241 tgctgtgttt ttcccgctac ccggatcaca tgaaacagca cgactttttc aaaagcgcta
      301 tgccggaagg ttacgttcaa gaacgtacca ttttctttaa agatgacggc aactacaaaa
      361 cccgcgccga agtcaaattt gaaggtgata cgctggtgaa ccgtattgaa ctgaaaggca
      421 tcgatttcaa agaagacggt aatatcctgg gccataaact ggaatataac tacaatagcc
      481 acaacgtgta tattatggcg gataaacaga aaaacggtat caaatctaac ttcaaaatcc
      541 gtcataatat cgaagatggc agtgttcagc tggccgacca ctatcagcaa aacaccccga
      601 ttggtgatgg cccggtcctg ctgccggaca atcattacct gagtacgcag tccaaactgt
      661 caaaagatcc gaacgaaaaa cgcgaccaca tggtcctgct ggaatttgtg accgcggccg
      721 gtatcacgct gggcatggat gaactgtaca aaggcggtgg cggttcgggc ggtggcggta
      781 gccagcaacg ttttgaatgg gaatttgaac agcaataata actcgag
//
