LOCUS       sequence-369140-       12937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing monSTIM1 in neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Kim S, Kyung T, Chung JH, Kim N, Keum S, Lee J, Park H, Kim HM, Lee 
            S, Shin HS, Do Heo W
  TITLE     Non-invasive optical control of endogenous Ca(2+) channels in awake 
            mice.
  JOURNAL   Nat Commun. 2020 Jan 10;11(1):210. doi: 10.1038/s41467-019-14005-4.
  PUBMED    31924789
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-10"; volume: "11(1):210. doi"; pages: " 
            10.1038/s41467-019-14005-4"
COMMENT     Sequence Label: pLenti-CamKIIa-monSTIM1
FEATURES             Location/Qualifiers
     source          1..12937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3900..3909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3906..3908,3909..3911,3912..4622)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3906..3908,3909..3911,3912..4622)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3951..3972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4212..4231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4559..4580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7629..8217
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7682..7702)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8100..8111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8220..8236)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8221..8237)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8742..8922
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8948..8965)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8954..9178
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9224..9652
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9311..9330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9521..9542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9661..9681)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9666..9995
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9846..9981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9908..9927
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10043..10090
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10109..10483
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10613..10734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10650..10669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10704..10723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10783..10799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10783..10799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10796..10818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10807..10823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10831..10837,10838..10855,10856..10861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10876..10897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11014..11031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11185..11773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11265..11284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11944..12735,12736..12804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12567..12586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12805..12909)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg
     3901 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3961 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4021 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     4081 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     4141 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4201 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4261 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4321 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4381 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4441 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4501 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4561 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4621 agccaccggt catgaagatg gacaaaaaga ccatcgtctg gtttcggaga gatttgagaa
     4681 tagaagataa tcccgcgctc gccgccgcgg cccacgaggg ttccgtcttc cccgttttca
     4741 tttggtgtcc tgaagaagaa ggccagtttt atcccggaag ggcctctagg tggtggatga
     4801 agcaaagtct ggcccatctt agccagtcac tgaaagcact gggcagtgat cttaccctga
     4861 tcaagacaca caataccatc tctgccatcc tcgactgcat cagggtgacc ggcgcaacga
     4921 aagtcgtgtt taaccacctg tacgatccag ttagtctggt gcgcgaccac actgtgaagg
     4981 agaagctggt ggaacggggg atcagtgtgc agagctacaa cggggacctt ctgtacgagc
     5041 catgggagat ctattgcgag aaagggaaac cgttcacctc cttcaacagt tactggaaga
     5101 aatgtttgga tatgtcaata gagtccgtta tgttgccccc tccctggaga ctgatgccga
     5161 ttactgctgc tgcagaggcc atctgggcct gctccatcga ggaactcggt ctggaaaatg
     5221 aagcagaaaa gccaagcaat gcacttctca ctagagcctg gagccccggc tggtctaatg
     5281 ccgacaagct gcttaacgag ttcatcgaaa aacaactgat tgactacgcg aagaactcca
     5341 agaaagtggt aggtaactca actagcttgc tctctccata tctccatttt ggcgagattt
     5401 ctgtccgcca tgtatttcag tgcgctcgga tgaaacagat tatctgggct cgcgataaaa
     5461 acagcgaagg cgccgaaagc gccgatctgt tcctgcgagg gatcggactt cgggaatact
     5521 cccggtatat atgtttcaac tttccattca cacacgagca gagtctgttg tcccacctca
     5581 ggttcttccc ctgggacgcc gatgtcgaca aattcaaggc atggagacag ggaaggacag
     5641 ggtacccact cgtggatgct ggcatgagag agctctgggc tacaggctgg atgcacaacc
     5701 gcatccgggt aatcgtgtcc tcatttgctg tcaagtttct gctcctgcct tggaaatggg
     5761 gaatgaagta cttttgggat acccttctcg acgccgactt ggagtgtgac attctgggat
     5821 ggcaatatat tagcgggtca attcctgacg gccatgagtt ggacaggttg gacaatccgg
     5881 ccttgcaggg agctaagtat gatcccgaag gagagtatat tcgacagtgg ctccccgagc
     5941 tggcccgact tcctacggag tggattcacc atccttggga cgcaccactg acagtgctca
     6001 aggcaagcgg ggtggagctg ggcaccaatt acgctaagcc tatagttgat atagatacag
     6061 cacgcgagct gctggctaaa gcgatctctc gcactcggga ggcgcagatt atgatcggtg
     6121 ctgccgccag ggaccccccc gacctcgaca acgaattgag tgggggaggc aaattcagtg
     6181 ggggaggcgg agggggtggt tcaggtggtg gtggtggttc gggtggtggc ggagggggag
     6241 gctccggact cagatctaag gagcacatga agaagatgat gaaggacttg gaggggttac
     6301 accgagctga gcagagtctg catgaccttc aggaaaggct gcacaaggcc caggaggagc
     6361 accgcacagt ggaggtggag aaggtccatc tggaaaagaa gctgcgcgat gagatcaacc
     6421 ttgctaagca ggaagcccag cggctgaagg agctgcggga gggtactgag aatgagcgga
     6481 gccgccaaaa atatgctgag gaggagttgg agcaggttcg ggaggccttg aggaaagcag
     6541 agaaggagct agaatctcac agctcatggt atgctccaga ggcccttcag aagtggctgc
     6601 agctgacaca tgaggtggag gtgcaatatt acaacatcaa gaagcaaaat gctgagaagc
     6661 agctgctggt ggccaaggag ggggctgaga agataaaaaa gaagagaaac acactctttg
     6721 gcaccttcca cgtggcccac agctcttccc tggatgatgt agatcataaa attctaacag
     6781 ctaagcaagc actgagcgag gtgacagcag cattgcggga gcgcctgcac cgctggcaac
     6841 agatcgagat cctctgtggc ttccagattg tcaacaaccc tggcatccac tcactggtgg
     6901 ctgccctcaa catagacccc agctggatgg gcagtacacg ccccaaccct gctcacttca
     6961 tcatgactga cgacgtggat gacatggatg aggagattgt gtctcccttg tccatgcagt
     7021 cccctagcct gcagagcagt gttcggcagc gcctgacgga gccacagcat ggcctgggat
     7081 ctcagagaga tttgacccat tccgattcgg agtcctccct ccacatgagt gaccgccagc
     7141 gtgtggcccc caaacctcct cagatgagcc gtgctgcaga cgaggctctc aatgccatga
     7201 cttccaatgg cagccaccgg ctgatcgagg gggtccaccc agggtctctg gtggagaaac
     7261 tgcctgacag ccctgccctg gccaagaagg cattactggc gctgaaccat gggctggaca
     7321 aggcccacag cctgatggag ctgagcccct cagccccacc tggtggctct ccacatttgg
     7381 attcttcccg ttctcacagc cccagctccc cagacccaga cacaccatct ccagttgggg
     7441 acagccgagc cctgcaagcc agccgaaaca cacgcattcc ccacctggct ggcaagaagg
     7501 ctgtggctga ggaggataat ggctctattg gcgaggaaac agactccagc ccaggccgga
     7561 agaagtttcc cctcaaaatc tttaagaagc ctcttaagaa gtaggaattc gatatcaagc
     7621 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7681 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7741 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7801 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7861 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7921 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7981 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     8041 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8101 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8161 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     8221 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     8281 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     8341 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8401 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     8461 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     8521 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8581 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     8641 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     8701 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8941 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9001 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9061 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9121 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9181 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9661 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9721 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9841 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9901 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9961 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
    10021 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
    10081 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    10141 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    10201 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    10261 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    10321 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    10381 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    10441 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    10501 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10561 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10621 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10681 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10741 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10861 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10921 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    11041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    11101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11821 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11881 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11941 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12001 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    12061 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12121 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12181 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12241 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12301 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12361 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12421 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12481 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12541 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12601 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12661 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12721 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12781 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12841 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12901 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
