LOCUS       sequence-369141-       12326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing monSTIM1 in astrocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12326)
  AUTHORS   Kim S, Kyung T, Chung JH, Kim N, Keum S, Lee J, Park H, Kim HM, Lee 
            S, Shin HS, Do Heo W
  TITLE     Non-invasive optical control of endogenous Ca(2+) channels in awake 
            mice.
  JOURNAL   Nat Commun. 2020 Jan 10;11(1):210. doi: 10.1038/s41467-019-14005-4.
  PUBMED    31924789
REFERENCE   2  (bases 1 to 12326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-10"; volume: "11(1):210. doi"; pages: " 
            10.1038/s41467-019-14005-4"
COMMENT     Sequence Label: pLenti-gfaABC1D-monSTIM1
FEATURES             Location/Qualifiers
     source          1..12326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        446..493
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             512..886
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1016..1137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1053..1072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1107..1126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1186..1202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1186..1202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1199..1221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1210..1226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1234..1240,1241..1258,1259..1264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1279..1300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1417..1434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1588..2176)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1668..1687)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2347..3138,3139..3207))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3208..3312)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3387..3406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3578..3957
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3959..4157
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4112..4132
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4175..4355
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4402..4527
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5020..5253
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5438..5482
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5780..5897
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      6629..6638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6635..6637,6638..6640,6641..7351)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6635..6637,6638..6640,6641..7351)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6680..6701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6941..6960)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7288..7309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10358..10946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10411..10431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10829..10840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10949..10965)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10950..10966)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11471..11651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11677..11694)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11683..11907
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11953..12326,1..55)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12040..12059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12250..12271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
      421 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
      481 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
      541 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
      601 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
      661 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
      721 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
      781 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
      841 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
      901 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
      961 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     1021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1141 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     1201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1321 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1381 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2221 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2281 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2341 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2401 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2461 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2521 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2581 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2641 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2701 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2761 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2821 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2881 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2941 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3001 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3061 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3121 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3181 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3241 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3301 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     3361 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3421 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     3481 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     3541 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     3601 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3661 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3721 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3781 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3841 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3901 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3961 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     4021 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     4081 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     4141 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
     4201 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4261 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4321 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     4381 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     4441 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     4501 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     4561 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     4621 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     4681 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     4741 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     4801 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     4861 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     4921 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     4981 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     5041 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     5101 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     5161 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     5221 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     5281 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     5341 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     5401 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     5461 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     5521 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     5581 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     5641 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     5701 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     5761 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     5821 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     5881 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta
     5941 aaacatatcc tggtgtggag taggggacgc tgctctgaca gaggctcggg ggcctgagct
     6001 ggctctgtga gctggggagg aggcagacag ccaggccttg tctgcaagca gacctggcag
     6061 cattgggctg gccgcccccc agggcctcct cttcatgccc agtgaatgac tcaccttggc
     6121 acagacacaa tgttcggggt gggcacagtg cctgcttccc gccgcacccc agcccccctc
     6181 aaatgccttc cgagaagccc attgagcagg gggcttgcat tgcaccccag cctgacagcc
     6241 tggcatcttg ggataaaagc agcacagccc cctaggggct gcccttgctg tgtggcgcca
     6301 ccggcggtgg agaacaaggc tctattcagc ctgtgcccag gaaaggggat caggggatgc
     6361 ccaggcatgg acagtgggtg gcaggggggg agaggagggc tgtctgcttc ccagaagtcc
     6421 aaggacacaa atgggtgagg ggagagctct ccccatagct gggctgcggc ccaaccccac
     6481 cccctcaggc tatgccaggg ggtgttgcca ggggcacccg ggcatcgcca gtctagccca
     6541 ctccttcaca aagccctcgc atcccaggag cgagcagagc cagagcaggt tggagaggag
     6601 acgcatcacc tccgctgctc gcggatccgc caccatggtg agcaagggcg aggagctgtt
     6661 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     6721 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     6781 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     6841 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     6901 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     6961 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7021 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     7081 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     7141 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     7201 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     7261 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     7321 gatcactctc ggcatggacg agctgtacaa gccaccggtc atgaagatgg acaaaaagac
     7381 catcgtctgg tttcggagag atttgagaat agaagataat cccgcgctcg ccgccgcggc
     7441 ccacgagggt tccgtcttcc ccgttttcat ttggtgtcct gaagaagaag gccagtttta
     7501 tcccggaagg gcctctaggt ggtggatgaa gcaaagtctg gcccatctta gccagtcact
     7561 gaaagcactg ggcagtgatc ttaccctgat caagacacac aataccatct ctgccatcct
     7621 cgactgcatc agggtgaccg gcgcaacgaa agtcgtgttt aaccacctgt acgatccagt
     7681 tagtctggtg cgcgaccaca ctgtgaagga gaagctggtg gaacggggga tcagtgtgca
     7741 gagctacaac ggggaccttc tgtacgagcc atgggagatc tattgcgaga aagggaaacc
     7801 gttcacctcc ttcaacagtt actggaagaa atgtttggat atgtcaatag agtccgttat
     7861 gttgccccct ccctggagac tgatgccgat tactgctgct gcagaggcca tctgggcctg
     7921 ctccatcgag gaactcggtc tggaaaatga agcagaaaag ccaagcaatg cacttctcac
     7981 tagagcctgg agccccggct ggtctaatgc cgacaagctg cttaacgagt tcatcgaaaa
     8041 acaactgatt gactacgcga agaactccaa gaaagtggta ggtaactcaa ctagcttgct
     8101 ctctccatat ctccattttg gcgagatttc tgtccgccat gtatttcagt gcgctcggat
     8161 gaaacagatt atctgggctc gcgataaaaa cagcgaaggc gccgaaagcg ccgatctgtt
     8221 cctgcgaggg atcggacttc gggaatactc ccggtatata tgtttcaact ttccattcac
     8281 acacgagcag agtctgttgt cccacctcag gttcttcccc tgggacgccg atgtcgacaa
     8341 attcaaggca tggagacagg gaaggacagg gtacccactc gtggatgctg gcatgagaga
     8401 gctctgggct acaggctgga tgcacaaccg catccgggta atcgtgtcct catttgctgt
     8461 caagtttctg ctcctgcctt ggaaatgggg aatgaagtac ttttgggata cccttctcga
     8521 cgccgacttg gagtgtgaca ttctgggatg gcaatatatt agcgggtcaa ttcctgacgg
     8581 ccatgagttg gacaggttgg acaatccggc cttgcaggga gctaagtatg atcccgaagg
     8641 agagtatatt cgacagtggc tccccgagct ggcccgactt cctacggagt ggattcacca
     8701 tccttgggac gcaccactga cagtgctcaa ggcaagcggg gtggagctgg gcaccaatta
     8761 cgctaagcct atagttgata tagatacagc acgcgagctg ctggctaaag cgatctctcg
     8821 cactcgggag gcgcagatta tgatcggtgc tgccgccagg gacccccccg acctcgacaa
     8881 cgaattgagt gggggaggca aattcagtgg gggaggcgga gggggtggtt caggtggtgg
     8941 tggtggttcg ggtggtggcg gagggggagg ctccggactc agatctaagg agcacatgaa
     9001 gaagatgatg aaggacttgg aggggttaca ccgagctgag cagagtctgc atgaccttca
     9061 ggaaaggctg cacaaggccc aggaggagca ccgcacagtg gaggtggaga aggtccatct
     9121 ggaaaagaag ctgcgcgatg agatcaacct tgctaagcag gaagcccagc ggctgaagga
     9181 gctgcgggag ggtactgaga atgagcggag ccgccaaaaa tatgctgagg aggagttgga
     9241 gcaggttcgg gaggccttga ggaaagcaga gaaggagcta gaatctcaca gctcatggta
     9301 tgctccagag gcccttcaga agtggctgca gctgacacat gaggtggagg tgcaatatta
     9361 caacatcaag aagcaaaatg ctgagaagca gctgctggtg gccaaggagg gggctgagaa
     9421 gataaaaaag aagagaaaca cactctttgg caccttccac gtggcccaca gctcttccct
     9481 ggatgatgta gatcataaaa ttctaacagc taagcaagca ctgagcgagg tgacagcagc
     9541 attgcgggag cgcctgcacc gctggcaaca gatcgagatc ctctgtggct tccagattgt
     9601 caacaaccct ggcatccact cactggtggc tgccctcaac atagacccca gctggatggg
     9661 cagtacacgc cccaaccctg ctcacttcat catgactgac gacgtggatg acatggatga
     9721 ggagattgtg tctcccttgt ccatgcagtc ccctagcctg cagagcagtg ttcggcagcg
     9781 cctgacggag ccacagcatg gcctgggatc tcagagagat ttgacccatt ccgattcgga
     9841 gtcctccctc cacatgagtg accgccagcg tgtggccccc aaacctcctc agatgagccg
     9901 tgctgcagac gaggctctca atgccatgac ttccaatggc agccaccggc tgatcgaggg
     9961 ggtccaccca gggtctctgg tggagaaact gcctgacagc cctgccctgg ccaagaaggc
    10021 attactggcg ctgaaccatg ggctggacaa ggcccacagc ctgatggagc tgagcccctc
    10081 agccccacct ggtggctctc cacatttgga ttcttcccgt tctcacagcc ccagctcccc
    10141 agacccagac acaccatctc cagttgggga cagccgagcc ctgcaagcca gccgaaacac
    10201 acgcattccc cacctggctg gcaagaaggc tgtggctgag gaggataatg gctctattgg
    10261 cgaggaaaca gactccagcc caggccggaa gaagtttccc ctcaaaatct ttaagaagcc
    10321 tcttaagaag taggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
    10381 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
    10441 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
    10501 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
    10561 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
    10621 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
    10681 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    10741 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
    10801 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    10861 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
    10921 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
    10981 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
    11041 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
    11101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
    11161 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
    11221 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
    11281 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
    11341 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
    11401 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
    11461 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    11521 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    11581 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    11641 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
    11701 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    11761 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    11821 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    11881 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
    11941 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
    12001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
    12061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    12121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
    12181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    12241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
    12301 cttttgattt ataagggatt ttgccg
//
