LOCUS       sequence-369197-        6115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Zamponi E, Meehl JB, Voeltz GK
  TITLE     The ER ladder is a unique morphological feature of developing 
            mammalian axons.
  JOURNAL   Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 
            10.1016/j.devcel.2022.05.002. Epub 2022 May 23.
  PUBMED    35609616
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2022.05.002"; journalName: "Dev Cell"; date: 
            "2022-06-6- 6"; volume: "57"; issue: "11"; pages: "1369-1382.e6"
COMMENT     Sequence Label: Rtn2b-mNeon
FEATURES             Location/Qualifiers
     source          1..6115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2005..2014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2011..2721
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      2266..2275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2843..2964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2880..2899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2934..2953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2971..3426)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3108..3129)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3320..3339
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3453..3557
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3559..3916
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3559..3750
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3582..3602)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3767..3902
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3951..4745
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3951..4745
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4005..4024)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4615..4634
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4933..4952)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4977..5024
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5353..5941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5842..5861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagat ggggcaggtc ctgccggtct tcgcccactg caaagaagct
      601 ccgtctacag cctcctcaac tcctgattcc acagaaggag ggaacgacga ctctgatttt
      661 cgagagctgc acacagcccg ggaattctca gaggaggacg aggaggagac cacgtcgcag
      721 gactggggca ccccccggga gctgaccttc tcctacatcg cctttgatgg tgtagtgggc
      781 tccgggggcc gcagggattc aactgcccgc cgcccccgcc cccagggccg ctcagtctcg
      841 gaaccacgag accagcaccc tcagcccagc ctgggcgaca gcttggagag catccccagc
      901 ctgagccaat ccccggagcc tggacgacgg ggtgatcctg acaccgcgcc tccatccgag
      961 cgccctctgg aagacctgag gcttcggttg gaccatctgg gctgggtggc ccggggaacg
     1021 ggatccgggg aggactcttc caccagcagc tccaccccgc tggaagacga agaaccccaa
     1081 gaacccaaca gattggagac aggagaagct ggggaagaac tggacctacg actccgactt
     1141 gctcagccct catcgcccga ggtcttgact ccccagctca gtccgggctc tgggacaccc
     1201 caggccggta ctccgtcccc atcccgatcg cgagattcga actctgggcc cgaagagcca
     1261 ttgctggaag aggaagaaaa gcagtggggg ccactggagc gagagccagt aaggggacag
     1321 tgcctcgata gcacggacca attagaattc acggtggagc cacgccttct agtggcggac
     1381 ctgctgtact ggaaggacac gaggacgtca ggagtggtct tcacaggcct gatggtctcc
     1441 ctcctctgcc tcctgcactt tagcatcgtg tccgtggccg cgcacttggc tctgttgctg
     1501 ctctgcggca ccatctctct cagggtttac cgcaaagtgc tgcaggccgt gcaccggggg
     1561 gatggagcca accctttcca ggcctacctg gatgtggacc tcaccctgac tcgggagcag
     1621 acggaacgtt tgtcccacca gatcacctcc cgcgtggtct cggcggccac gcagctgcgg
     1681 cacttcttcc tggtagaaga cctcgtggat tccctcaagc tggccctcct cttctacatc
     1741 ttgaccttcg tgggtgccat cttcaatggt ttgactcttc tcattctggg agtgattggt
     1801 ctattcacca tccccctgct gtaccggcag caccaggctc agatcgacca atatgtgggg
     1861 ttggtgacca atcagttgag ccacatcaaa gctaagatcc gagctaaaat cccagggacc
     1921 ggagccctgg cctctgcagc agccgcagtc tccggatcca aagccaaagc cgaacgggta
     1981 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg
     2041 gcctctctcc cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt
     2101 gacatggtgg gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag
     2161 tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc
     2221 ttccatcagt acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat
     2281 ggctccggct accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt
     2341 aactaccgct acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact
     2401 ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg
     2461 tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc
     2521 actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca
     2581 atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag
     2641 cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc
     2701 atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
     2761 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2821 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2941 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3001 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3061 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3121 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3181 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3241 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3301 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3361 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3421 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3481 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3541 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3601 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3661 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3781 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3841 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3901 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3961 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4021 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4081 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4141 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4201 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4261 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4321 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4381 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4441 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4501 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4561 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4621 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4681 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4741 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4801 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4861 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     4921 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     4981 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5041 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5101 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5161 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5221 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5281 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6001 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
     6061 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
