LOCUS       sequence-369212-       15374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15374)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 15374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDONR221-HUWE1_delta3291-3298
FEATURES             Location/Qualifiers
     source          1..15374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(13603..13702)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13814..13833)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13825..14634
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13892..13911)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      14724..15312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15213..15232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atgaaagtag acaggactaa actgaagaag acacctactg aggctcctgc agactgcaga
      541 gccttaatag acaaactcaa agtttgtaat gatgagcaac ttctcttgga actgcagcag
      601 atcaaaacat ggaacattgg aaagtgcgag ttatatcact gggtggacct gttggaccgc
      661 ttcgatggaa tactggcaga tgctggacag acagtggaga atatgtcatg gatgctcgta
      721 tgtgataggc cagaaagaga gcaactgaaa atgcttctct tggctgtgtt gaacttcaca
      781 gccttgctca ttgagtacag cttttcccgg catctgtaca gttccataga gcatttgaca
      841 actttattgg cttcctctga tatgcaagtg gtgctggcag tcctcaatct cctatatgta
      901 tttagcaaaa gatcaaacta catcactcgt ctgggatctg acaagaggac cccgctgcta
      961 actcggctac aacatttggc agagagctgg ggtggaaagg agaatggctt tggacttgca
     1021 gaatgttgca gagacttgca tatgatgaaa tatccaccca gtgcaactac actacacttt
     1081 gaattctatg cagatcctgg ggccgaggtc aaaattgaga aaaggacaac tagtaacaca
     1141 ctacattata ttcacataga gcaacttgac aagatttcag aaagcccttc tgaaatcatg
     1201 gaatctctta ccaaaatgta cagcattcct aaggataagc agatgctgtt atttacacac
     1261 atacgactgg cccatggctt ttctaatcac aggaagcgat tgcaggcagt tcaggccaga
     1321 ctgcatgcaa tatctatatt agtgtattcc aatgccttgc aggaatcagc aaacagtatc
     1381 ttgtataatg gcttgataga ggagttagtg gacgtgctgc aaataacgga taagcagctt
     1441 atggagatta aagcagcttc tttacgaaca ttaacatcaa ttgtccactt ggagagaact
     1501 cccaaactca gcagtattat tgactgtact ggaactgcct cctaccatgg atttttgcca
     1561 gtgcttgtaa ggaactgtat ccaggccatg attgatcctt ccatggatcc ataccctcac
     1621 cagtttgcca ctgctctctt ctctttttta taccatctgg ccagctacga tgctggtggt
     1681 gaagccttgg tctcctgtgg aatgatggaa gccttattga aggtcataaa gtttcttggc
     1741 gatgaacagg accagataac atttgtcacc agagccgtca gagtggttga ccttatcacc
     1801 aacctggata tggcagcttt tcaatcccat agtggacttt ctatcttcat ttatagactt
     1861 gagcatgaag tagatctgtg ccgaaaagaa tgtccgtttg tgatcaagcc aaagatccag
     1921 agacccaata ctacacaaga aggagaggaa atggaaactg atatggatgg agtccagtgt
     1981 attccacaac gagcagcact tctgaaatcc atgttgaatt tcctcaagaa ggccatccaa
     2041 gaccctgctt tctcagatgg catacgacat gtgatggatg gttctctgcc tacctccctg
     2101 aaacacatca tcagcaatgc agaatactat ggcccatcac tcttcctcct agctactgaa
     2161 gtggtgactg tgtttgtatt tcaagaacca tcactgctct cctcactcca ggacaatgga
     2221 ttgacagatg tcatgctgca tgcactgctt atcaaagatg ttcctgctac ccgtgaagtc
     2281 cttggctccc tcccaaatgt attcagtgca ctctgtttga atgcccgagg tcttcagtct
     2341 tttgttcagt gtcagccttt tgaacgcctc ttcaaagttc ttctgtctcc agattacctc
     2401 ccagccatgc ggaggaggag aagttctgat ccccttgggg atactgcatc caacctgggg
     2461 agtgctgtcg atgagctcat gagacatcag cccaccctta aaacagatgc aacgactgcc
     2521 atcatcaagt tacttgaaga aatctgtaat cttggaaggg accccaaata catctgtcag
     2581 aagccatcga tccagaaggc agatggcact gccactgctc ctcccccaag gtctaatcat
     2641 gccgcagaag aagcctctag tgaggatgag gaggaagagg aagtacaggc catgcagagc
     2701 tttaattcta cccagcaaaa tgaaactgag cctaatcagc aggttgttgg tacagaggaa
     2761 cgtattccta ttcccctcat ggattacatc cttaatgtga tgaaatttgt ggaatctatt
     2821 ctgagcaaca atacaacaga tgaccactgc caggaatttg tgaatcagaa aggactgttg
     2881 cctttggtta ccattttggg tcttcccaat ctgcccattg actttcccac atctgctgcc
     2941 tgtcaggctg ttgcaggtgt ctgcaaatcc atattgacac tgtcacatga acccaaagtc
     3001 cttcaagagg gtctccttca gttggactcc atcctctcct ccctggagcc cttacaccgc
     3061 cccattgaat cccctggggg ctcagtgttg ttgcgagaac tggcttgcgc aggcaatgtt
     3121 gctgatgcta ccctctcagc ccaggccaca cctctgctgc atgcactcac tgctgcccat
     3181 gcctacatca tgatgtttgt tcatacttgc agagttggac agagtgaaat tcgttccatc
     3241 tccgtaaacc agtggggctc tcaattgggt ctgagtgttt tgagcaagct gagccagtta
     3301 tactgttccc tggtgtggga aagcactgtc ctcctctctc tgtgtacccc aaacagccta
     3361 ccatctgggt gtgaatttgg ccaggcagat atgcagaaac tggttccaaa ggatgagaag
     3421 gcaggtacga cccagggcgg aaaaagatca gatggggaac aggatggagc agctggaagt
     3481 atggatgctt ctacccaggg cttattagaa ggcattgggc tagatggtga cacattggct
     3541 cccatggaga cagatgaacc tactgcttca gactctaagg gcaaatctaa aatcacacca
     3601 gcaatggctg ccagaattaa gcaaatcaag cctttgttat cagcttcctc cagattaggc
     3661 cgagcacttg ctgagctatt tggacttctt gttaaacttt gtgtgggatc tcctgtccgc
     3721 cagagaagga gccatcatgc tgccagcacc actacagcac cgacacctgc cgcgcgatca
     3781 acagcctcag ctctcactaa gctcttgact aaggggttat cttggcagcc cccaccatat
     3841 acacctactc cccgattcag gctgacattc ttcatctgtt cagttggttt cacatcccca
     3901 atgctgtttg atgagaggaa gtatccctac cacctcatgc tgcaaaaatt tctctgctcc
     3961 ggaggccaca atgctctttt tgaaactttc aactgggctc tgtccatggg aggtaaagtt
     4021 cctgtttctg agggattgga acactcagac ttgcctgatg gcacaggaga attcctagat
     4081 gcctggctta tgctggtgga gaagatggtg aatcccacca cggtgcttga atctccacat
     4141 tcgctgcctg ccaaattgcc tggaggtgtc cagaactttc cccagttcag tgcactgcgc
     4201 ttccttgtgg taactcagaa agcagccttt acttgcatca aaaacttatg gaaccggaaa
     4261 cccctgaagg tatatggtgg acgaatggct gaatcgatgc tggccattct atgccacatc
     4321 ctccgaggag aacctgtgat tcgagagaga ctaagcaagg agaaggaggg gtctcgagga
     4381 gaagaggata cagggcaaga ggaaggtggc tcccgccggg aacctcaagt caaccagcaa
     4441 caactgcaac agctcatgga catgggcttc acaagggaac atgcaatgga ggcactgttg
     4501 aacaccagca ccatggagca ggccacagag taccttttaa cccaccctcc tccaatcatg
     4561 ggaggagttg ttcgggatct cagcatgtct gaagaggacc agatgatgag agcaattgct
     4621 atgtctctgg gacaggatat tccaatggat caaagggcag agtcacctga ggaagttgct
     4681 tgccggaagg aggaagagga acggaaagct cgggaaaagc aggaggagga agaggctaaa
     4741 tgtctagaga agttccagga tgctgacccg ttggaacaag atgagctcca cactttcaca
     4801 gatactatgt tgccaggctg cttccacctt cttgatgagc tgccagacac agtataccgt
     4861 gtgtgtgacc tgatcatgac agcaatcaaa cgtaatggag cagattatcg tgacatgatt
     4921 ctgaagcaag tagtcaatca ggtgtgggaa gctgctgatg tattgatcaa agctgctctt
     4981 cccctgacaa caagtgacac aaaaaccgtg tcagagtgga taagtcagat ggccacactg
     5041 ccccaggcct ccaatttggc tactagaatc ttgcttttaa cgctactttt tgaggagttg
     5101 aagctacctt gtgcttgggt ggttgaatca agtggcatcc ttaatgtcct aatcaaactc
     5161 ttggaagtgg ttcagccctg cctccaggca gccaaggagc agaaggaagt ccagacccca
     5221 aagtggatca caccagtgtt gctcctgatt gatttctatg aaaagacagc catctcctca
     5281 aaaaggagag cccagatgac taagtacctg caatccaaca gcaacaactg gcgctggttt
     5341 gatgatcgct ctgggcgttg gtgtagttac agtgcaagca acaatagcac tattgattct
     5401 gcctggaaat ctggagagac aagcgtgcga ttcactgcag gccgaagaag atacacggtc
     5461 caattcacta caatggtgca ggttaatgag gaaacaggga accgacgccc tgtgatgctg
     5521 actctcctca gggtacctcg gctgaataaa aattcaaaaa acagcaatgg acaggaacta
     5581 gagaagacgc tggaagaaag caaagaaatg gatatcaaac gtaaagaaaa taaaggcaat
     5641 gatacccctt tggccctaga gagtacaaac actgaaaagg agacaagcct ggaggaaaca
     5701 aaaatcgggg agatcctgat ccagggcttg acagaagata tggtgactgt tttaatccgg
     5761 gcctgcgtga gcatgctggg agtccctgtg gacccagata ctttgcatgc cacccttcgt
     5821 ctctgtctga ggctcacccg ggaccacaaa tatgccatga tgtttgcaga actgaagagt
     5881 acccgcatga tcttgaattt gacccagagc tcaggcttca atgggtttac tcccctggtc
     5941 acccttctct taagacacat cattgaggac ccctgtaccc ttcgtcatac catggaaaag
     6001 gttgttcgct cagcagctac aagtggagct ggtagcacta cctctggtgt tgtgtctggc
     6061 agcctcggct ctcgggagat caactacatc cttcgtgtcc ttgggccagc cgcatgccgc
     6121 aatccagaca tattcacaga agtggccaac tgctgtatcc gcatcgccct tcctgcccct
     6181 cgaggctcag gaactgcttc agatgatgaa tttgagaatc ttagaattaa aggccctaat
     6241 gctgtacagc tggtgaagac cacccctttg aagccctcac ctctgcctgt catccctgat
     6301 actatcaagg aagtgatcta tgatatgctg aatgctctgg ctgcatacca tgctccagag
     6361 gaagccgata aatctgatcc taaacctggg gttatgaccc aagaggttgg ccagctcctg
     6421 caagacatgg gtgatgatgt ataccagcag taccggtcac ttacgcgtca gagcagtgac
     6481 tttgatacgc agtcaggttt ttccattaat agtcaggtct ttgctgcaga tggtgcctcc
     6541 actgagactt ccgcatctgg gacctcccaa ggagaggctt caactccaga ggagtctcga
     6601 gatgggaaga aagataaaga aggggaccgg gcctctgagg aaggcaaaca gaaaggcaag
     6661 ggcagcaaac ctttaatgcc tacctccact atccttcgtc ttctggcaga gttggtgagg
     6721 tcctatgttg gtattgctac cctgattgcc aactacagct acactgtggg ccagtctgaa
     6781 ctgatcaaag aggactgcag tgtgctagct tttgttctgg accacctgct cccacatacc
     6841 cagaatgcag aagacaagga cacccctgcc ttggcccgcc tgttcctcgc aagcctggct
     6901 gctgcaggga gtggcacaga tgcccaggtg gccctagtga atgaagtaaa agcagccctt
     6961 ggacgggcac tggctatggc tgagagtaca gagaaacatg ccaggcttca ggcagtgatg
     7021 tgtatcatca gtactatcat ggagtcctgc ccctccacct ccagcttcta cagcagtgcc
     7081 acagcgaaga cccagcacaa tggcatgaac aacatcattc ggcttttcct gaagaaggga
     7141 ctggttaatg acctggccag agtacctcac agcttagacc tgtccagtcc caacatggcc
     7201 aacacagtca atgctgctct gaagcctttg gaaacacttt cccggattgt gaaccagccc
     7261 agtagccttt ttggcagcaa gagtgcttct agcaagaaca agtctgagca ggatgcccaa
     7321 ggagcctctc aagattccag tagcaaccag caggacccag gcgagcctgg ggaagcagaa
     7381 gtgcaggagg aggatcatga tgtcactcag acagaggtgg cagatgggga tatcatggat
     7441 ggggaggctg aaaccgactc agtggtgatt gctgggcagc ctgaggtgct cagttcacaa
     7501 gagatgcagg ttgagaatga gctggaggac ctgatagatg agttgcttga gagggatggc
     7561 ggatctggga acagtacaat tatagtgagc agaagtggag aggatgaatc acaagaggac
     7621 gtgctgatgg atgaagctcc ttccaacctc agccaagctt ccaccttgca ggccaaccga
     7681 gaagattcca tgaatatcct ggaccctgag gatgaggagg agcacactca ggaagaggac
     7741 agcagtggca gtaacgagga tgaggatgat agtcaggatg aagaggagga ggaggaggaa
     7801 gatgaggaag atgatcagga ggatgatgaa ggtgaagagg gagatgaaga cgatgacgac
     7861 gatggctctg agatggaatt ggatgaggat tatcctgata tgaacgcttc tcccttggtc
     7921 cgatttgagc gctttgaccg ggaggatgat ctcatcattg agtttgacaa catgttctcc
     7981 agtgctacag acatcccccc atccccagga aatatcccta ccacccatcc actgatggtg
     8041 cgccatgcag accacagttc tctgacactg ggcagtggct cttcaacaac tcgtctcacc
     8101 cagggcatcg ggcgcagtca gaggacccta aggcagctga cggccaatac tggccacacc
     8161 attcatgttc actaccctgg gaatcgccag cccaaccctc ctcttatact gcagaggttg
     8221 cttggtccct cagctgctgc tgacatcctt cagctgagca gcagccttcc cctacaaagc
     8281 cggggtcggg cccgcctcct ggtaggcaac gatgacgtcc acatcatcgc ccgttctgat
     8341 gatgagctgc tggatgactt tttccatgat cagagcacag ctaccagcca agcaggaacc
     8401 ctgtccagca tccccacagc cctgacccgc tggacagaag aatgcaaagt tctcgatgct
     8461 gagagcatgc atgactgtgt ttcagtggtt aaagtgtcca ttgtcaatca cctggaattc
     8521 ctgagggatg aggagctgga agaaaggcga gagaagcgca ggaaacaact ggctgaggaa
     8581 gaaacaaaga taactgataa aggcaaagaa gataaggaga acagggatca gagtgcacag
     8641 tgtactgcat ctaagtcaaa tgactccact gaacagaatc tctcagatgg gacgcctatg
     8701 cctgacagct acccaacaac cccatcttca actgatgcag ctacatctga gtccaaggag
     8761 acccttggca ctctgcaatc ctcacaacag caaccaacac tcccaacccc accagctttg
     8821 ggagaggttc ctcaggagct gcagtctcca gctggagaag ggggcagctc tacacagcta
     8881 ttgatgcctg tagagccaga ggaattgggt cccacaaggc caagtgggga agcagaaaca
     8941 actcagatgg agttatcccc agctcccact ataacctcac tttccccaga gagagctgag
     9001 gattctgatg cactgacggc tgtcagcagt cagctagaag gctctcctat ggatacaagc
     9061 agcctggctt cctgtacctt agaggaggct gtgggtgaca cttcagcagc tggcagttct
     9121 gagcagccca gagcaggcag ctccactcct ggggatgccc caccagctgt ggcggaagtg
     9181 caaggcagga gtgatgggtc aggggaatct gcccagccac ctgaggacag ctccccacct
     9241 gcatcctctg agagctcttc caccagagat tctgccgtgg ccatttctgg cgccgattct
     9301 cggggaatac tggaagagcc actgccatct acctctagtg aggaagaaga tcctcttgcc
     9361 ggtatatcat tgcccgaggg ggtagacccg tctttcctcg ccgccctccc ggacgatatt
     9421 cggagagaag tacttcaaaa tcaactcgga attcggcccc ccactcgcac tgccccgagt
     9481 actaatagct cagcgcccgc cgttgtcggg aatccaggag tcactgaagt gagtccggag
     9541 ttccttgcag cactcccgcc ggctattcag gaagaagtcc ttgcgcaaca gcgagcggaa
     9601 caacagaggc gagaacttgc ccaaaacgca tcttcagaca ctccaatgga ccctgtcacg
     9661 ttcatacaga cccttccctc cgacttgcga cgatccgtgc tggaagacat ggaagattct
     9721 gtacttgccg taatgccgcc tgatattgcg gcagaagcac aagctcttcg aagggaacaa
     9781 gaagcaaggc agcgacagtt gatgcatgag aggctcttcg gtcactcctc aacctccgcg
     9841 ttgtctgcca tactcagatc acccgcgttc acaagccgcc tctcaggaaa tcggggcgtc
     9901 cagtacacca gattggccgt ccaacgaggc ggcacgtttc aaatgggcgg gtctagctca
     9961 cataataggc caagcggctc aaatgtagat actctgctgc gacttcgggg acgactgctg
    10021 ttggatcacg aagcgctctc ctgtctcttg gttctgctct tcgttgatga gcctaagctc
    10081 aacacaagta ggctccatcg cgtactgcgg aatttgtgct atcatgcgca gacacgccat
    10141 tgggtaatta ggagcttgct ttcaatcctg cagcgaagtt cagaatccga actttgcata
    10201 gaaacaccaa agcttaccac ttccgaggaa aaggggaaaa agtcttcaaa atcttgcggt
    10261 agcagctcac atgaaaatcg cccgctcgat cttttgcaca aaatggaaag caagtctagt
    10321 aatcagctct cctggctttc cgtttccatg ggatctggga atatatttca gattcagaga
    10381 tctggtggcc gcaagcatac agaaaagcat gcttctggtg gaagtacggt acacatacac
    10441 cctcaggcag cccctgtcgt atgcaggcac gtgttggaca cactcatcca gctcgctaag
    10501 gtctttccct cccattttac tcagcaacga acgaaggaaa ccaattgcga gagtgaccgc
    10561 gaaagaggga acaaagcgtg ctctccgtgt tcttcccagt cttcaagtag cggcatttgt
    10621 actgattttt gggatctgct tgtgaaactg gataacatga acgtttccag aaaggggaaa
    10681 aactccgtca aatctgtccc ggtgtctgcg ggaggcgagg gtgagacatc cccttactct
    10741 ctggaggcat cacccctggg acaacttatg aatatgctca gccaccctgt gatacgaaga
    10801 tcctcacttc tgacggaaaa gctgctcagg ctgctttcac tgatttccat agcacttcct
    10861 gagaacaagg tgagtgaggc acaggcaaat tccgggtctg gagcttcttc cacaacgact
    10921 gcgacctcca caaccagcac aacaactacg actgctgcgt ctaccacccc tactccccct
    10981 acggcgccta ctcccgtcac atccgcgccg gccctcgtag ccgctacggc gataagtacg
    11041 atcgtcgttg cggccagtac tacggtcacc acaccgacca cagcgaccac gacggtcagt
    11101 attagtccta cgactaaggg gtcaaagagc cctgctaaag tgtccgacgg aggatcctcc
    11161 tcaacagatt ttaaaatggt gtcctctgga cttacggaaa accagctgca gctttccgta
    11221 gaggtactta cctcacattc ctgcagcgag gaaggactcg aagacgctgc gaatgtgctc
    11281 ctgcagctga gtagaggaga ttccggtacc agggataccg ttctgaaatt gcttcttaat
    11341 ggcgccaggc acctcgggta cactctgtgc aagcaaattg gtactctgtt ggccgaactc
    11401 cgagaataca atctggagca gcaacgacgg gcgcagtgtg aaacactgag tcccgacggg
    11461 cttcctgagg aacagccgca gacaacgaaa cttaagggta aaatgcagag tcggttcgac
    11521 atggcagaaa atgtcgtgat agtggcttct cagaaacgcc ctctcggggg aagggaactg
    11581 cagctgccca gtatgtccat gcttacttcc aagacaagca cccagaagtt ttttttgaga
    11641 gtacttcaag tcataattca attgagagac gatactcgcc gagccaacaa gaaagccaaa
    11701 caaacgggaa ggctcggttc ttctggtctg ggctcagcat catccatcca ggcggccgtg
    11761 cggcaacttg aagcagaggc agacgccatc atacaaatgg tacgtgaggg tcaaagggcg
    11821 cggagacagc aacaagcagc aacgtcggag tctagccagt cagaggcgtc tgtccggagg
    11881 gaggaatcac ccatggatgt ggaccagcca tctcccagtg ctcaagatac tcaatccatt
    11941 gcctccgatg gaaccccaca gggggagaag gaaaaggaag aaagaccacc tgagttaccc
    12001 ctgctcagcg agcagctgag tttggacgag ctgtgggaca tgcttgggga gtgtctaaag
    12061 gaactagagg aatcccatga ccagcatgcg gtgctagtgc tacagcctgc tgtcgaggcc
    12121 ttctttctgg tccatgccac agagcgggag agcaagcctc ctgtccgaga cacccgtgag
    12181 agccagctgg cacacatcaa ggacgagcct cctccactct cccctgcccc cttaacccca
    12241 gccacgcctt cctcccttga cccattcttc tcccgggagc cctcatctat gcacatctcc
    12301 tcaagcctgc cccctgacac acagaagttc cttcgctttg cagagactca ccgcactgtg
    12361 ttaaaccaga tcctacggca gtccacgacc caccttgctg atgggccttt tgctgtcctg
    12421 gtagactaca ttcgtgtcct cgactttgat gtcaagcgca aatatttccg ccaagagctg
    12481 gagcgtttag atgaggggct ccggaaagaa gacatggctg tgcatgtccg tcgtgaccat
    12541 gtgtttgaag actcctatcg tgagctgcat cgcaaatccc ccgaagaaat gaagaatcga
    12601 ttgtatatag tatttgaagg agaagaaggg caggatgctg gcgggctcct gcgggagtgg
    12661 tatatgatca tctctcgaga gatgtttaac cctatgtatg ccttgttccg tacctcacct
    12721 ggtgatcgag tcacctacac catcaatcca tcttcccact gcaaccccaa ccatctgtcc
    12781 tattttaaat ttgtcggacg cattgtggcc aaagctgtat atgacaaccg tcttctggag
    12841 tgctacttta ctcgatcctt ttacaaacac atcttgggca agtcagtcag atatacagat
    12901 atggagagtg aagattacca cttctaccaa ggtctggttt atctgctgga aaatgatgtc
    12961 tccacactag gctatgacct caccttcagc actgaggtcc aagagtttgg agtttgtgaa
    13021 gttcgtgacc tcaaacccaa tggggccaac atcttggtaa cagaggagaa taagaaggag
    13081 tatgtacacc tggtatgcca gatgagaatg acaggagcca tccgcaagca gttggcggct
    13141 ttcttagaag gcttctatga gatcattcca aagcgcctca tttccatctt cactgagcag
    13201 gagttagagc tgcttatatc aggactgccc accattgaca tcgatgatct gaaatccaac
    13261 actgaatacc acaagtacca gtccaactct attcagatcc agtggttctg gagagcattg
    13321 cgttctttcg atcaagctga ccgtgccaag ttcctccagt ttgtcacggg tacttccaag
    13381 gtacccctgc aaggctttgc tgccctcgaa ggcatgaatg gcattcagaa gtttcagatc
    13441 catcgagatg ataggtccac agatcgcctg ccttcagctc acacatgttt taatcagctg
    13501 gatctgcctg cctatgagag ctttgagaag ctccgccaca tgctactgtt ggctatccag
    13561 gagtgctctg aaggctttgg gctggcctaa taagcggccg cgacccagct ttcttgtaca
    13621 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
    13681 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
    13741 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
    13801 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
    13861 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
    13921 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
    13981 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
    14041 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
    14101 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
    14161 gattcaggtg aaaatattgt tgatgcgctg gcagtgtccc tgcgccggtt gcattcgatt
    14221 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
    14281 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
    14341 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
    14401 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
    14461 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
    14521 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
    14581 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
    14641 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc
    14701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    14761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    14821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    14881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    14941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    15001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    15061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    15121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    15181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    15241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    15301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    15361 tttgctcaca tgtt
//
