LOCUS       sequence-369223-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. 
            mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Niwa S, Chiba K
  TITLE     Generation of recombinant and chickenized scFv versions of an 
            anti-kinesin monoclonal antibody H2.
  JOURNAL   Cytoskeleton (Hoboken). 2023 Apr 10. doi: 10.1002/cm.21756.
  PUBMED    37036074
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Cytoskeleton (Hoboken). 2023 Apr 10. doi: 10.1002/cm.21756."
COMMENT     Sequence Label: pSN879
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           759..785
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           849..890
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           897..923
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           987..1028
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           1035..1061
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           1125..1166
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcat ggacgagctg tacaagtaag ctaagcactt cgtggccgtc
      721 gatcgtttaa agggaggtag tgagtcgacc agtggatcct ggaggcttgc tgaaggctgt
      781 atgctgttct tgtgacttct ggtcgtagtt ttggccactg actgactacg accaagtcac
      841 aagaacagga cacaaggcct gttactagca ctcacatgga acaaatggcc cagatcctgg
      901 aggcttgctg aaggctgtat gctgttcatt tgcagtctga accttgtttt ggccactgac
      961 tgacaaggtt cactgcaaat gaacaggaca caaggcctgt tactagcact cacatggaac
     1021 aaatggccca gatcctggag gcttgctgaa ggctgtatgc tgaaatcggc aaagcacctt
     1081 gatgttttgg ccactgactg acatcaaggt tttgccgatt tcaggacaca aggcctgtta
     1141 ctagcactca catggaacaa atggcc
//
