LOCUS       sequence-369243-       15392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 15392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDONR221-HUWE1_H3962D
FEATURES             Location/Qualifiers
     source          1..15392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(13621..13720)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13832..13851)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13843..14652
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13910..13929)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      14742..15330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15231..15250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 accatgaaag tagacaggac taaactgaag aagacaccta ctgaggctcc tgcagactgc
      541 agagccttaa tagacaaact caaagtttgt aatgatgagc aacttctctt ggaactgcag
      601 cagatcaaaa catggaacat tggaaagtgc gagttatatc actgggtgga cctgttggac
      661 cgcttcgatg gaatactggc agatgctgga cagacagtgg agaatatgtc atggatgctc
      721 gtatgtgata ggccagaaag agagcaactg aaaatgcttc tcttggctgt gttgaacttc
      781 acagccttgc tcattgagta cagcttttcc cggcatctgt acagttccat agagcatttg
      841 acaactttat tggcttcctc tgatatgcaa gtggtgctgg cagtcctcaa tctcctatat
      901 gtatttagca aaagatcaaa ctacatcact cgtctgggat ctgacaagag gaccccgctg
      961 ctaactcggc tacaacattt ggcagagagc tggggtggaa aggagaatgg ctttggactt
     1021 gcagaatgtt gcagagactt gcatatgatg aaatatccac ccagtgcaac tacactacac
     1081 tttgaattct atgcagatcc tggggccgag gtcaaaattg agaaaaggac aactagtaac
     1141 acactacatt atattcacat agagcaactt gacaagattt cagaaagccc ttctgaaatc
     1201 atggaatctc ttaccaaaat gtacagcatt cctaaggata agcagatgct gttatttaca
     1261 cacatacgac tggcccatgg cttttctaat cacaggaagc gattgcaggc agttcaggcc
     1321 agactgcatg caatatctat attagtgtat tccaatgcct tgcaggaatc agcaaacagt
     1381 atcttgtata atggcttgat agaggagtta gtggacgtgc tgcaaataac ggataagcag
     1441 cttatggaga ttaaagcagc ttctttacga acattaacat caattgtcca cttggagaga
     1501 actcccaaac tcagcagtat tattgactgt actggaactg cctcctacca tggatttttg
     1561 ccagtgcttg taaggaactg tatccaggcc atgattgatc cttccatgga tccataccct
     1621 caccagtttg ccactgctct cttctctttt ttataccatc tggccagcta cgatgctggt
     1681 ggtgaagcct tggtctcctg tggaatgatg gaagccttat tgaaggtcat aaagtttctt
     1741 ggcgatgaac aggaccagat aacatttgtc accagagccg tcagagtggt tgaccttatc
     1801 accaacctgg atatggcagc ttttcaatcc catagtggac tttctatctt catttataga
     1861 cttgagcatg aagtagatct gtgccgaaaa gaatgtccgt ttgtgatcaa gccaaagatc
     1921 cagagaccca atactacaca agaaggagag gaaatggaaa ctgatatgga tggagtccag
     1981 tgtattccac aacgagcagc acttctgaaa tccatgttga atttcctcaa gaaggccatc
     2041 caagaccctg ctttctcaga tggcatacga catgtgatgg atggttctct gcctacctcc
     2101 ctgaaacaca tcatcagcaa tgcagaatac tatggcccat cactcttcct cctagctact
     2161 gaagtggtga ctgtgtttgt atttcaagaa ccatcactgc tctcctcact ccaggacaat
     2221 ggattgacag atgtcatgct gcatgcactg cttatcaaag atgttcctgc tacccgtgaa
     2281 gtccttggct ccctcccaaa tgtattcagt gcactctgtt tgaatgcccg aggtcttcag
     2341 tcttttgttc agtgtcagcc ttttgaacgc ctcttcaaag ttcttctgtc tccagattac
     2401 ctcccagcca tgcggaggag gagaagttct gatccccttg gggatactgc atccaacctg
     2461 gggagtgctg tcgatgagct catgagacat cagcccaccc ttaaaacaga tgcaacgact
     2521 gccatcatca agttacttga agaaatctgt aatcttggaa gggaccccaa atacatctgt
     2581 cagaagccat cgatccagaa ggcagatggc actgccactg ctcctccccc aaggtctaat
     2641 catgccgcag aagaagcctc tagtgaggat gaggaggaag aggaagtaca ggccatgcag
     2701 agctttaatt ctacccagca aaatgaaact gagcctaatc agcaggttgt tggtacagag
     2761 gaacgtattc ctattcccct catggattac atccttaatg tgatgaaatt tgtggaatct
     2821 attctgagca acaatacaac agatgaccac tgccaggaat ttgtgaatca gaaaggactg
     2881 ttgcctttgg ttaccatttt gggtcttccc aatctgccca ttgactttcc cacatctgct
     2941 gcctgtcagg ctgttgcagg tgtctgcaaa tccatattga cactgtcaca tgaacccaaa
     3001 gtccttcaag agggtctcct tcagttggac tccatcctct cctccctgga gcccttacac
     3061 cgccccattg aatcccctgg gggctcagtg ttgttgcgag aactggcttg cgcaggcaat
     3121 gttgctgatg ctaccctctc agcccaggcc acacctctgc tgcatgcact cactgctgcc
     3181 catgcctaca tcatgatgtt tgttcatact tgcagagttg gacagagtga aattcgttcc
     3241 atctccgtaa accagtgggg ctctcaattg ggtctgagtg ttttgagcaa gctgagccag
     3301 ttatactgtt ccctggtgtg ggaaagcact gtcctcctct ctctgtgtac cccaaacagc
     3361 ctaccatctg ggtgtgaatt tggccaggca gatatgcaga aactggttcc aaaggatgag
     3421 aaggcaggta cgacccaggg cggaaaaaga tcagatgggg aacaggatgg agcagctgga
     3481 agtatggatg cttctaccca gggcttatta gaaggcattg ggctagatgg tgacacattg
     3541 gctcccatgg agacagatga acctactgct tcagactcta agggcaaatc taaaatcaca
     3601 ccagcaatgg ctgccagaat taagcaaatc aagcctttgt tatcagcttc ctccagatta
     3661 ggccgagcac ttgctgagct atttggactt cttgttaaac tttgtgtggg atctcctgtc
     3721 cgccagagaa ggagccatca tgctgccagc accactacag caccgacacc tgccgcgcga
     3781 tcaacagcct cagctctcac taagctcttg actaaggggt tatcttggca gcccccacca
     3841 tatacaccta ctccccgatt caggctgaca ttcttcatct gttcagttgg tttcacatcc
     3901 ccaatgctgt ttgatgagag gaagtatccc taccacctca tgctgcaaaa atttctctgc
     3961 tccggaggcc acaatgctct ttttgaaact ttcaactggg ctctgtccat gggaggtaaa
     4021 gttcctgttt ctgagggatt ggaacactca gacttgcctg atggcacagg agaattccta
     4081 gatgcctggc ttatgctggt ggagaagatg gtgaatccca ccacggtgct tgaatctcca
     4141 cattcgctgc ctgccaaatt gcctggaggt gtccagaact ttccccagtt cagtgcactg
     4201 cgcttccttg tggtaactca gaaagcagcc tttacttgca tcaaaaactt atggaaccgg
     4261 aaacccctga aggtatatgg tggacgaatg gctgaatcga tgctggccat tctatgccac
     4321 atcctccgag gagaacctgt gattcgagag agactaagca aggagaagga ggggtctcga
     4381 ggagaagagg atacagggca agaggaaggt ggctcccgcc gggaacctca agtcaaccag
     4441 caacaactgc aacagctcat ggacatgggc ttcacaaggg aacatgcaat ggaggcactg
     4501 ttgaacacca gcaccatgga gcaggccaca gagtaccttt taacccaccc tcctccaatc
     4561 atgggaggag ttgttcggga tctcagcatg tctgaagagg accagatgat gagagcaatt
     4621 gctatgtctc tgggacagga tattccaatg gatcaaaggg cagagtcacc tgaggaagtt
     4681 gcttgccgga aggaggaaga ggaacggaaa gctcgggaaa agcaggagga ggaagaggct
     4741 aaatgtctag agaagttcca ggatgctgac ccgttggaac aagatgagct ccacactttc
     4801 acagatacta tgttgccagg ctgcttccac cttcttgatg agctgccaga cacagtatac
     4861 cgtgtgtgtg acctgatcat gacagcaatc aaacgtaatg gagcagatta tcgtgacatg
     4921 attctgaagc aagtagtcaa tcaggtgtgg gaagctgctg atgtattgat caaagctgct
     4981 cttcccctga caacaagtga cacaaaaacc gtgtcagagt ggataagtca gatggccaca
     5041 ctgccccagg cctccaattt ggctactaga atcttgcttt taacgctact ttttgaggag
     5101 ttgaagctac cttgtgcttg ggtggttgaa tcaagtggca tccttaatgt cctaatcaaa
     5161 ctcttggaag tggttcagcc ctgcctccag gcagccaagg agcagaagga agtccagacc
     5221 ccaaagtgga tcacaccagt gttgctcctg attgatttct atgaaaagac agccatctcc
     5281 tcaaaaagga gagcccagat gactaagtac ctgcaatcca acagcaacaa ctggcgctgg
     5341 tttgatgatc gctctgggcg ttggtgtagt tacagtgcaa gcaacaatag cactattgat
     5401 tctgcctgga aatctggaga gacaagcgtg cgattcactg caggccgaag aagatacacg
     5461 gtccaattca ctacaatggt gcaggttaat gaggaaacag ggaaccgacg ccctgtgatg
     5521 ctgactctcc tcagggtacc tcggctgaat aaaaattcaa aaaacagcaa tggacaggaa
     5581 ctagagaaga cgctggaaga aagcaaagaa atggatatca aacgtaaaga aaataaaggc
     5641 aatgataccc ctttggccct agagagtaca aacactgaaa aggagacaag cctggaggaa
     5701 acaaaaatcg gggagatcct gatccagggc ttgacagaag atatggtgac tgttttaatc
     5761 cgggcctgcg tgagcatgct gggagtccct gtggacccag atactttgca tgccaccctt
     5821 cgtctctgtc tgaggctcac ccgggaccac aaatatgcca tgatgtttgc agaactgaag
     5881 agtacccgca tgatcttgaa tttgacccag agctcaggct tcaatgggtt tactcccctg
     5941 gtcacccttc tcttaagaca catcattgag gacccctgta cccttcgtca taccatggaa
     6001 aaggttgttc gctcagcagc tacaagtgga gctggtagca ctacctctgg tgttgtgtct
     6061 ggcagcctcg gctctcggga gatcaactac atccttcgtg tccttgggcc agccgcatgc
     6121 cgcaatccag acatattcac agaagtggcc aactgctgta tccgcatcgc ccttcctgcc
     6181 cctcgaggct caggaactgc ttcagatgat gaatttgaga atcttagaat taaaggccct
     6241 aatgctgtac agctggtgaa gaccacccct ttgaagccct cacctctgcc tgtcatccct
     6301 gatactatca aggaagtgat ctatgatatg ctgaatgctc tggctgcata ccatgctcca
     6361 gaggaagccg ataaatctga tcctaaacct ggggttatga cccaagaggt tggccagctc
     6421 ctgcaagaca tgggtgatga tgtataccag cagtaccggt cacttacgcg tcagagcagt
     6481 gactttgata cgcagtcagg tttttccatt aatagtcagg tctttgctgc agatggtgcc
     6541 tccactgaga cttccgcatc tgggacctcc caaggagagg cttcaactcc agaggagtct
     6601 cgagatggga agaaagataa agaaggggac cgggcctctg aggaaggcaa acagaaaggc
     6661 aagggcagca aacctttaat gcctacctcc actatccttc gtcttctggc agagttggtg
     6721 aggtcctatg ttggtattgc taccctgatt gccaactaca gctacactgt gggccagtct
     6781 gaactgatca aagaggactg cagtgtgcta gcttttgttc tggaccacct gctcccacat
     6841 acccagaatg cagaagacaa ggacacccct gccttggccc gcctgttcct cgcaagcctg
     6901 gctgctgcag ggagtggcac agatgcccag gtggccctag tgaatgaagt aaaagcagcc
     6961 cttggacggg cactggctat ggctgagagt acagagaaac atgccaggct tcaggcagtg
     7021 atgtgtatca tcagtactat catggagtcc tgcccctcca cctccagctt ctacagcagt
     7081 gccacagcga agacccagca caatggcatg aacaacatca ttcggctttt cctgaagaag
     7141 ggactggtta atgacctggc cagagtacct cacagcttag acctgtccag tcccaacatg
     7201 gccaacacag tcaatgctgc tctgaagcct ttggaaacac tttcccggat tgtgaaccag
     7261 cccagtagcc tttttggcag caagagtgct tctagcaaga acaagtctga gcaggatgcc
     7321 caaggagcct ctcaagattc cagtagcaac cagcaggacc caggcgagcc tggggaagca
     7381 gaagtgcagg aggaggatca tgatgtcact cagacagagg tggcagatgg ggatatcatg
     7441 gatggggagg ctgaaaccga ctcagtggtg attgctgggc agcctgaggt gctcagttca
     7501 caagagatgc aggttgagaa tgagctggag gacctgatag atgagttgct tgagagggat
     7561 ggcggatctg ggaacagtac aattatagtg agcagaagtg gagaggatga atcacaagag
     7621 gacgtgctga tggatgaagc tccttccaac ctcagccaag cttccacctt gcaggccaac
     7681 cgagaagatt ccatgaatat cctggaccct gaggatgagg aggagcacac tcaggaagag
     7741 gacagcagtg gcagtaacga ggatgaggat gatagtcagg atgaagagga ggaggaggag
     7801 gaagatgagg aagatgatca ggaggatgat gaaggtgaag agggagatga agacgatgac
     7861 gacgatggct ctgagatgga attggatgag gattatcctg atatgaacgc ttctcccttg
     7921 gtccgatttg agcgctttga ccgggaggat gatctcatca ttgagtttga caacatgttc
     7981 tccagtgcta cagacatccc cccatcccca ggaaatatcc ctaccaccca tccactgatg
     8041 gtgcgccatg cagaccacag ttctctgaca ctgggcagtg gctcttcaac aactcgtctc
     8101 acccagggca tcgggcgcag tcagaggacc ctaaggcagc tgacggccaa tactggccac
     8161 accattcatg ttcactaccc tgggaatcgc cagcccaacc ctcctcttat actgcagagg
     8221 ttgcttggtc cctcagctgc tgctgacatc cttcagctga gcagcagcct tcccctacaa
     8281 agccggggtc gggcccgcct cctggtaggc aacgatgacg tccacatcat cgcccgttct
     8341 gatgatgagc tgctggatga ctttttccat gatcagagca cagctaccag ccaagcagga
     8401 accctgtcca gcatccccac agccctgacc cgctggacag aagaatgcaa agttctcgat
     8461 gctgagagca tgcatgactg tgtttcagtg gttaaagtgt ccattgtcaa tcacctggaa
     8521 ttcctgaggg atgaggagct ggaagaaagg cgagagaagc gcaggaaaca actggctgag
     8581 gaagaaacaa agataactga taaaggcaaa gaagataagg agaacaggga tcagagtgca
     8641 cagtgtactg catctaagtc aaatgactcc actgaacaga atctctcaga tgggacgcct
     8701 atgcctgaca gctacccaac aaccccatct tcaactgatg cagctacatc tgagtccaag
     8761 gagacccttg gcactctgca atcctcacaa cagcaaccaa cactcccaac cccaccagct
     8821 ttgggagagg ttcctcagga gctgcagtct ccagctggag aagggggcag ctctacacag
     8881 ctattgatgc ctgtagagcc agaggaattg ggtcccacaa ggccaagtgg ggaagcagaa
     8941 acaactcaga tggagttatc cccagctccc actataacct cactttcccc agagagagct
     9001 gaggattctg atgcactgac ggctgtcagc agtcagctag aaggctctcc tatggataca
     9061 agcagcctgg cttcctgtac cttagaggag gctgtgggtg acacttcagc agctggcagt
     9121 tctgagcagc ccagagcagg cagctccact cctggggatg ccccaccagc tgtggcggaa
     9181 gtgcaaggca ggagtgatgg gtcaggggaa tctgcccagc cacctgagga cagctcccca
     9241 cctgcatcct ctgagagctc ttccaccaga gattctgccg tggccatttc tggcgccgat
     9301 tcccgaggaa tcctagaaga gccgttgcct tcaacaagca gtgaagaaga agatcccctt
     9361 gcgggtatca gtctccctga aggtgtggac ccctcttttc tggctgccct gcctgatgac
     9421 atccgtcggg aagttctaca gaaccagcta ggcattcgtc caccaacccg gactgccccc
     9481 tccacaaata gctcagcgcc tgcagtggtg gggaatcctg gtgtgactga ggtgagtccc
     9541 gagtttcttg cggcactgcc acccgcgatc caagaggaag tactggcaca gcagagagct
     9601 gagcagcagc gacgagaact agcacagaat gccagctcag acacccctat ggaccctgtg
     9661 accttcatcc agactctgcc ctcagacctg cgccgtagtg tcctagagga tatggaggac
     9721 agtgtgttag ctgtgatgcc acctgacatt gcagctgagg ctcaagccct gagacgagag
     9781 caagaagccc ggcagcgaca gctcatgcat gagcgtctgt ttgggcacag tagcacctcc
     9841 gcactctctg ctattctccg aagcccggct ttcaccagtc gcttaagtgg caaccgtggg
     9901 gtccagtata ctcgccttgc tgtgcagaga ggtggcacct tccagatggg gggtagcagc
     9961 agccataaca ggccttctgg cagtaatgta gatactctcc tccgcctccg aggacggctc
    10021 cttctggacc acgaagccct ttcttgtctc ttggtcctac tttttgtgga tgagccaaag
    10081 ctcaatacta gccgtctaca ccgagtactg agaaatctct gctaccatgc ccagacccgc
    10141 cactgggtca tccgcagtct gctctccatc ttgcagcgca gcagtgagag tgagctatgc
    10201 attgaaacac ccaaactcac tacaagtgag gaaaagggca aaaagtcgag caagagctgt
    10261 gggtcaagta gccatgagaa ccgtcccctg gacctgctac acaagatgga gtcaaagagc
    10321 tccaaccagc tttcctggct ctcagtatcc atggatgcag ccctaggctg caggactaat
    10381 atatttcaga tccagcgttc aggggggcgt aaacataccg agaagcatgc aagcggtggc
    10441 tccaccgtcc acatccatcc ccaagctgct cctgttgtct gcagacacgt tttggataca
    10501 ctcattcaat tggccaaggt atttcccagc cacttcacac agcagcggac caaagaaaca
    10561 aactgtgaga gtgatcggga aaggggcaat aaggcctgta gcccatgctc ctcacagtcc
    10621 tccagcagtg gcatttgcac agacttctgg gacttattgg taaaactgga caacatgaat
    10681 gtcagccgga aaggcaagaa ctccgtgaag tcagtgccag tgagcgctgg cggtgagggg
    10741 gaaacctctc catacagcct cgaggcctct ccactggggc agctcatgaa catgttgtca
    10801 cacccagtca tccgccggag ctctctctta actgagaaac tcctcagact cctttctctc
    10861 atctcaattg ctctcccaga aaacaaggtg tcagaagcac aggctaattc tggcagcggt
    10921 gcttcctcca ccaccactgc cacctcaacc acatctacca ccaccaccac tgccgcctcc
    10981 accacgccca caccccctac tgcacccacc cctgtcactt ctgctccagc cctggttgct
    11041 gccacggcta tttccaccat tgtcgtagct gcttcgacca cagtgactac ccccacgact
    11101 gctaccacta ctgtttcaat ttctcccact actaagggca gcaaatctcc agcgaaggtg
    11161 agtgatgggg gcagcagcag tacagacttt aagatggtgt cctctggcct cactgaaaac
    11221 cagctacagc tctctgtaga ggtgttgaca tcccactctt gttctgagga aggcttagag
    11281 gatgcagcca acgtactact gcagctctcc cggggggact ctgggacccg ggacactgtt
    11341 ctcaagctgc tactgaatgg agcccgccat ctgggttata ccctttgtaa acaaataggt
    11401 accctgctgg ccgagctgcg ggaatacaac ctcgagcagc agcggcgagc ccaatgtgaa
    11461 accctctctc ctgatggcct gcctgaggag cagccacaga ccaccaagct gaagggcaaa
    11521 atgcagagca ggtttgacat ggctgagaat gtggtaattg tggcatctca gaagcgacct
    11581 ttgggtggcc gggagctcca gctgccttct atgtccatgt tgacatccaa gacatctacc
    11641 cagaagttct tcttgagggt actacaggtc atcatccagc tccgggacga cacgcgccgg
    11701 gctaacaaga aagccaagca gacaggcagg ctaggttcct ccggtttagg ctcagctagc
    11761 agcatccagg cagctgttcg gcagctggag gctgaggctg atgccattat acaaatggta
    11821 cgtgagggtc aaagggcgcg gagacagcaa caagcagcaa cgtcggagtc tagccagtca
    11881 gaggcgtctg tccggaggga ggaatcaccc atggatgtgg accagccatc tcccagtgct
    11941 caagatactc aatccattgc ctccgatgga accccacagg gggagaagga aaaggaagaa
    12001 agaccacctg agttacccct gctcagcgag cagctgagtt tggacgagct gtgggacatg
    12061 cttggggagt gtctaaagga actagaggaa tcccatgacc agcatgcggt gctagtgcta
    12121 cagcctgctg tcgaggcctt ctttctggtc catgccacag agcgggagag caagcctcct
    12181 gtccgagaca cccgtgagag ccagctggca cacatcaagg acgagcctcc tccactctcc
    12241 cctgccccct taaccccagc cacgccttcc tcccttgacc cattcttctc ccgggagccc
    12301 tcatctatgc acatctcctc aagcctgccc cctgacacac agaagttcct tcgctttgca
    12361 gagactgacc gcactgtgtt aaaccagatc ctacggcagt ccacgaccca ccttgctgat
    12421 gggccttttg ctgtcctggt agactacatt cgtgtcctcg actttgatgt caagcgcaaa
    12481 tatttccgcc aagagctgga gcgtttagat gaggggctcc ggaaagaaga catggctgtg
    12541 catgtccgtc gtgaccatgt gtttgaagac tcctatcgtg agctgcatcg caaatccccc
    12601 gaagaaatga agaatcgatt gtatatagta tttgaaggag aagaagggca ggatgctggc
    12661 gggctcctgc gggagtggta tatgatcatc tctcgagaga tgtttaaccc tatgtatgcc
    12721 ttgttccgta cctcacctgg tgatcgagtc acctacacca tcaatccatc ttcccactgc
    12781 aaccccaacc atctgtccta ttttaaattt gtcggacgca ttgtggccaa agctgtatat
    12841 gacaaccgtc ttctggagtg ctactttact cgatcctttt acaaacacat cttgggcaag
    12901 tcagtcagat atacagatat ggagagtgaa gattaccact tctaccaagg tctggtttat
    12961 ctgctggaaa atgatgtctc cacactaggc tatgacctca ccttcagcac tgaggtccaa
    13021 gagtttggag tttgtgaagt tcgtgacctc aaacccaatg gggccaacat cttggtaaca
    13081 gaggagaata agaaggagta tgtacacctg gtatgccaga tgagaatgac aggagccatc
    13141 cgcaagcagt tggcggcttt cttagaaggc ttctatgaga tcattccaaa gcgcctcatt
    13201 tccatcttca ctgagcagga gttagagctg cttatatcag gactgcccac cattgacatc
    13261 gatgatctga aatccaacac tgaataccac aagtaccagt ccaactctat tcagatccag
    13321 tggttctgga gagcattgcg ttctttcgat caagctgacc gtgccaagtt cctccagttt
    13381 gtcacgggta cttccaaggt acccctgcaa ggctttgctg ccctcgaagg catgaatggc
    13441 attcagaagt ttcagatcca tcgagatgac aggtccacag atcgcctgcc ttcagctcac
    13501 acatgtttta atcagctgga tctgcctgcc tatgagagct ttgagaagct ccgccacatg
    13561 ctactgttgg ctatccagga gtgctctgaa ggctttgggc tggcctaata agcggccgcg
    13621 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
    13681 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
    13741 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
    13801 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
    13861 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
    13921 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
    13981 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
    14041 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
    14101 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
    14161 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgtccctg
    14221 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
    14281 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
    14341 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
    14401 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
    14461 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
    14521 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
    14581 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
    14641 gagtttttct aatcagaatt ggttaattgg ttgtaacatt attcagattg ggccccgttc
    14701 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    14761 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    14821 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    14881 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    14941 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    15001 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    15061 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    15121 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    15181 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    15241 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    15301 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    15361 ctggcctttt gctggccttt tgctcacatg tt
//
