LOCUS       sequence-369246-       15326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15326)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 15326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDONR221-HUWE1_delta169-189
FEATURES             Location/Qualifiers
     source          1..15326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(13555..13654)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13766..13785)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13777..14586
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13844..13863)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      14676..15264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15165..15184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atgaaagtag acaggactaa actgaagaag acacctactg aggctcctgc agactgcaga
      541 gccttaatag acaaactcaa agtttgtaat gatgagcaac ttctcttgga actgcagcag
      601 atcaaaacat ggaacattgg aaagtgcgag ttatatcact gggtggacct gttggaccgc
      661 ttcgatggaa tactggcaga tgctggacag acagtggaga atatgtcatg gatgctcgta
      721 tgtgataggc cagaaagaga gcaactgaaa atgcttctct tggctgtgtt gaacttcaca
      781 gccttgctca ttgagtacag cttttcccgg catctgtaca gttccataga gcatttgaca
      841 actttattgg cttcctctga tatgcaagtg gtgctggcag tcctcaatct cctatatgta
      901 tttagcaaaa gatcaaacta catcactcgt ctgggatctg acaagaggac cccgctgcta
      961 actcggctac aacatttggc agagaaatat ccacccagtg caactacact acactttgaa
     1021 ttctatgcag atcctggggc cgaggtcaaa attgagaaaa ggacaactag taacacacta
     1081 cattatattc acatagagca acttgacaag atttcagaaa gcccttctga aatcatggaa
     1141 tctcttacca aaatgtacag cattcctaag gataagcaga tgctgttatt tacacacata
     1201 cgactggccc atggcttttc taatcacagg aagcgattgc aggcagttca ggccagactg
     1261 catgcaatat ctatattagt gtattccaat gccttgcagg aatcagcaaa cagtatcttg
     1321 tataatggct tgatagagga gttagtggac gtgctgcaaa taacggataa gcagcttatg
     1381 gagattaaag cagcttcttt acgaacatta acatcaattg tccacttgga gagaactccc
     1441 aaactcagca gtattattga ctgtactgga actgcctcct accatggatt tttgccagtg
     1501 cttgtaagga actgtatcca ggccatgatt gatccttcca tggatccata ccctcaccag
     1561 tttgccactg ctctcttctc ttttttatac catctggcca gctacgatgc tggtggtgaa
     1621 gccttggtct cctgtggaat gatggaagcc ttattgaagg tcataaagtt tcttggcgat
     1681 gaacaggacc agataacatt tgtcaccaga gccgtcagag tggttgacct tatcaccaac
     1741 ctggatatgg cagcttttca atcccatagt ggactttcta tcttcattta tagacttgag
     1801 catgaagtag atctgtgccg aaaagaatgt ccgtttgtga tcaagccaaa gatccagaga
     1861 cccaatacta cacaagaagg agaggaaatg gaaactgata tggatggagt ccagtgtatt
     1921 ccacaacgag cagcacttct gaaatccatg ttgaatttcc tcaagaaggc catccaagac
     1981 cctgctttct cagatggcat acgacatgtg atggatggtt ctctgcctac ctccctgaaa
     2041 cacatcatca gcaatgcaga atactatggc ccatcactct tcctcctagc tactgaagtg
     2101 gtgactgtgt ttgtatttca agaaccatca ctgctctcct cactccagga caatggattg
     2161 acagatgtca tgctgcatgc actgcttatc aaagatgttc ctgctacccg tgaagtcctt
     2221 ggctccctcc caaatgtatt cagtgcactc tgtttgaatg cccgaggtct tcagtctttt
     2281 gttcagtgtc agccttttga acgcctcttc aaagttcttc tgtctccaga ttacctccca
     2341 gccatgcgga ggaggagaag ttctgatccc cttggggata ctgcatccaa cctggggagt
     2401 gctgtcgatg agctcatgag acatcagccc acccttaaaa cagatgcaac gactgccatc
     2461 atcaagttac ttgaagaaat ctgtaatctt ggaagggacc ccaaatacat ctgtcagaag
     2521 ccatcgatcc agaaggcaga tggcactgcc actgctcctc ccccaaggtc taatcatgcc
     2581 gcagaagaag cctctagtga ggatgaggag gaagaggaag tacaggccat gcagagcttt
     2641 aattctaccc agcaaaatga aactgagcct aatcagcagg ttgttggtac agaggaacgt
     2701 attcctattc ccctcatgga ttacatcctt aatgtgatga aatttgtgga atctattctg
     2761 agcaacaata caacagatga ccactgccag gaatttgtga atcagaaagg actgttgcct
     2821 ttggttacca ttttgggtct tcccaatctg cccattgact ttcccacatc tgctgcctgt
     2881 caggctgttg caggtgtctg caaatccata ttgacactgt cacatgaacc caaagtcctt
     2941 caagagggtc tccttcagtt ggactccatc ctctcctccc tggagccctt acaccgcccc
     3001 attgaatccc ctgggggctc agtgttgttg cgagaactgg cttgcgcagg caatgttgct
     3061 gatgctaccc tctcagccca ggccacacct ctgctgcatg cactcactgc tgcccatgcc
     3121 tacatcatga tgtttgttca tacttgcaga gttggacaga gtgaaattcg ttccatctcc
     3181 gtaaaccagt ggggctctca attgggtctg agtgttttga gcaagctgag ccagttatac
     3241 tgttccctgg tgtgggaaag cactgtcctc ctctctctgt gtaccccaaa cagcctacca
     3301 tctgggtgtg aatttggcca ggcagatatg cagaaactgg ttccaaagga tgagaaggca
     3361 ggtacgaccc agggcggaaa aagatcagat ggggaacagg atggagcagc tggaagtatg
     3421 gatgcttcta cccagggctt attagaaggc attgggctag atggtgacac attggctccc
     3481 atggagacag atgaacctac tgcttcagac tctaagggca aatctaaaat cacaccagca
     3541 atggctgcca gaattaagca aatcaagcct ttgttatcag cttcctccag attaggccga
     3601 gcacttgctg agctatttgg acttcttgtt aaactttgtg tgggatctcc tgtccgccag
     3661 agaaggagcc atcatgctgc cagcaccact acagcaccga cacctgccgc gcgatcaaca
     3721 gcctcagctc tcactaagct cttgactaag gggttatctt ggcagccccc accatataca
     3781 cctactcccc gattcaggct gacattcttc atctgttcag ttggtttcac atccccaatg
     3841 ctgtttgatg agaggaagta tccctaccac ctcatgctgc aaaaatttct ctgctccgga
     3901 ggccacaatg ctctttttga aactttcaac tgggctctgt ccatgggagg taaagttcct
     3961 gtttctgagg gattggaaca ctcagacttg cctgatggca caggagaatt cctagatgcc
     4021 tggcttatgc tggtggagaa gatggtgaat cccaccacgg tgcttgaatc tccacattcg
     4081 ctgcctgcca aattgcctgg aggtgtccag aactttcccc agttcagtgc actgcgcttc
     4141 cttgtggtaa ctcagaaagc agcctttact tgcatcaaaa acttatggaa ccggaaaccc
     4201 ctgaaggtat atggtggacg aatggctgaa tcgatgctgg ccattctatg ccacatcctc
     4261 cgaggagaac ctgtgattcg agagagacta agcaaggaga aggaggggtc tcgaggagaa
     4321 gaggatacag ggcaagagga aggtggctcc cgccgggaac ctcaagtcaa ccagcaacaa
     4381 ctgcaacagc tcatggacat gggcttcaca agggaacatg caatggaggc actgttgaac
     4441 accagcacca tggagcaggc cacagagtac cttttaaccc accctcctcc aatcatggga
     4501 ggagttgttc gggatctcag catgtctgaa gaggaccaga tgatgagagc aattgctatg
     4561 tctctgggac aggatattcc aatggatcaa agggcagagt cacctgagga agttgcttgc
     4621 cggaaggagg aagaggaacg gaaagctcgg gaaaagcagg aggaggaaga ggctaaatgt
     4681 ctagagaagt tccaggatgc tgacccgttg gaacaagatg agctccacac tttcacagat
     4741 actatgttgc caggctgctt ccaccttctt gatgagctgc cagacacagt ataccgtgtg
     4801 tgtgacctga tcatgacagc aatcaaacgt aatggagcag attatcgtga catgattctg
     4861 aagcaagtag tcaatcaggt gtgggaagct gctgatgtat tgatcaaagc tgctcttccc
     4921 ctgacaacaa gtgacacaaa aaccgtgtca gagtggataa gtcagatggc cacactgccc
     4981 caggcctcca atttggctac tagaatcttg cttttaacgc tactttttga ggagttgaag
     5041 ctaccttgtg cttgggtggt tgaatcaagt ggcatcctta atgtcctaat caaactcttg
     5101 gaagtggttc agccctgcct ccaggcagcc aaggagcaga aggaagtcca gaccccaaag
     5161 tggatcacac cagtgttgct cctgattgat ttctatgaaa agacagccat ctcctcaaaa
     5221 aggagagccc agatgactaa gtacctgcaa tccaacagca acaactggcg ctggtttgat
     5281 gatcgctctg ggcgttggtg tagttacagt gcaagcaaca atagcactat tgattctgcc
     5341 tggaaatctg gagagacaag cgtgcgattc actgcaggcc gaagaagata cacggtccaa
     5401 ttcactacaa tggtgcaggt taatgaggaa acagggaacc gacgccctgt gatgctgact
     5461 ctcctcaggg tacctcggct gaataaaaat tcaaaaaaca gcaatggaca ggaactagag
     5521 aagacgctgg aagaaagcaa agaaatggat atcaaacgta aagaaaataa aggcaatgat
     5581 acccctttgg ccctagagag tacaaacact gaaaaggaga caagcctgga ggaaacaaaa
     5641 atcggggaga tcctgatcca gggcttgaca gaagatatgg tgactgtttt aatccgggcc
     5701 tgcgtgagca tgctgggagt ccctgtggac ccagatactt tgcatgccac ccttcgtctc
     5761 tgtctgaggc tcacccggga ccacaaatat gccatgatgt ttgcagaact gaagagtacc
     5821 cgcatgatct tgaatttgac ccagagctca ggcttcaatg ggtttactcc cctggtcacc
     5881 cttctcttaa gacacatcat tgaggacccc tgtacccttc gtcataccat ggaaaaggtt
     5941 gttcgctcag cagctacaag tggagctggt agcactacct ctggtgttgt gtctggcagc
     6001 ctcggctctc gggagatcaa ctacatcctt cgtgtccttg ggccagccgc atgccgcaat
     6061 ccagacatat tcacagaagt ggccaactgc tgtatccgca tcgcccttcc tgcccctcga
     6121 ggctcaggaa ctgcttcaga tgatgaattt gagaatctta gaattaaagg ccctaatgct
     6181 gtacagctgg tgaagaccac ccctttgaag ccctcacctc tgcctgtcat ccctgatact
     6241 atcaaggaag tgatctatga tatgctgaat gctctggctg cataccatgc tccagaggaa
     6301 gccgataaat ctgatcctaa acctggggtt atgacccaag aggttggcca gctcctgcaa
     6361 gacatgggtg atgatgtata ccagcagtac cggtcactta cgcgtcagag cagtgacttt
     6421 gatacgcagt caggtttttc cattaatagt caggtctttg ctgcagatgg tgcctccact
     6481 gagacttccg catctgggac ctcccaagga gaggcttcaa ctccagagga gtctcgagat
     6541 gggaagaaag ataaagaagg ggaccgggcc tctgaggaag gcaaacagaa aggcaagggc
     6601 agcaaacctt taatgcctac ctccactatc cttcgtcttc tggcagagtt ggtgaggtcc
     6661 tatgttggta ttgctaccct gattgccaac tacagctaca ctgtgggcca gtctgaactg
     6721 atcaaagagg actgcagtgt gctagctttt gttctggacc acctgctccc acatacccag
     6781 aatgcagaag acaaggacac ccctgccttg gcccgcctgt tcctcgcaag cctggctgct
     6841 gcagggagtg gcacagatgc ccaggtggcc ctagtgaatg aagtaaaagc agcccttgga
     6901 cgggcactgg ctatggctga gagtacagag aaacatgcca ggcttcaggc agtgatgtgt
     6961 atcatcagta ctatcatgga gtcctgcccc tccacctcca gcttctacag cagtgccaca
     7021 gcgaagaccc agcacaatgg catgaacaac atcattcggc ttttcctgaa gaagggactg
     7081 gttaatgacc tggccagagt acctcacagc ttagacctgt ccagtcccaa catggccaac
     7141 acagtcaatg ctgctctgaa gcctttggaa acactttccc ggattgtgaa ccagcccagt
     7201 agcctttttg gcagcaagag tgcttctagc aagaacaagt ctgagcagga tgcccaagga
     7261 gcctctcaag attccagtag caaccagcag gacccaggcg agcctgggga agcagaagtg
     7321 caggaggagg atcatgatgt cactcagaca gaggtggcag atggggatat catggatggg
     7381 gaggctgaaa ccgactcagt ggtgattgct gggcagcctg aggtgctcag ttcacaagag
     7441 atgcaggttg agaatgagct ggaggacctg atagatgagt tgcttgagag ggatggcgga
     7501 tctgggaaca gtacaattat agtgagcaga agtggagagg atgaatcaca agaggacgtg
     7561 ctgatggatg aagctccttc caacctcagc caagcttcca ccttgcaggc caaccgagaa
     7621 gattccatga atatcctgga ccctgaggat gaggaggagc acactcagga agaggacagc
     7681 agtggcagta acgaggatga ggatgatagt caggatgaag aggaggagga ggaggaagat
     7741 gaggaagatg atcaggagga tgatgaaggt gaagagggag atgaagacga tgacgacgat
     7801 ggctctgaga tggaattgga tgaggattat cctgatatga acgcttctcc cttggtccga
     7861 tttgagcgct ttgaccggga ggatgatctc atcattgagt ttgacaacat gttctccagt
     7921 gctacagaca tccccccatc cccaggaaat atccctacca cccatccact gatggtgcgc
     7981 catgcagacc acagttctct gacactgggc agtggctctt caacaactcg tctcacccag
     8041 ggcatcgggc gcagtcagag gaccctaagg cagctgacgg ccaatactgg ccacaccatt
     8101 catgttcact accctgggaa tcgccagccc aaccctcctc ttatactgca gaggttgctt
     8161 ggtccctcag ctgctgctga catccttcag ctgagcagca gccttcccct acaaagccgg
     8221 ggtcgggccc gcctcctggt aggcaacgat gacgtccaca tcatcgcccg ttctgatgat
     8281 gagctgctgg atgacttttt ccatgatcag agcacagcta ccagccaagc aggaaccctg
     8341 tccagcatcc ccacagccct gacccgctgg acagaagaat gcaaagttct cgatgctgag
     8401 agcatgcatg actgtgtttc agtggttaaa gtgtccattg tcaatcacct ggaattcctg
     8461 agggatgagg agctggaaga aaggcgagag aagcgcagga aacaactggc tgaggaagaa
     8521 acaaagataa ctgataaagg caaagaagat aaggagaaca gggatcagag tgcacagtgt
     8581 actgcatcta agtcaaatga ctccactgaa cagaatctct cagatgggac gcctatgcct
     8641 gacagctacc caacaacccc atcttcaact gatgcagcta catctgagtc caaggagacc
     8701 cttggcactc tgcaatcctc acaacagcaa ccaacactcc caaccccacc agctttggga
     8761 gaggttcctc aggagctgca gtctccagct ggagaagggg gcagctctac acagctattg
     8821 atgcctgtag agccagagga attgggtccc acaaggccaa gtggggaagc agaaacaact
     8881 cagatggagt tatccccagc tcccactata acctcacttt ccccagagag agctgaggat
     8941 tctgatgcac tgacggctgt cagcagtcag ctagaaggct ctcctatgga tacaagcagc
     9001 ctggcttcct gtaccttaga ggaggctgtg ggtgacactt cagcagctgg cagttctgag
     9061 cagcccagag caggcagctc cactcctggg gatgccccac cagctgtggc ggaagtgcaa
     9121 ggcaggagtg atgggtcagg ggaatctgcc cagccacctg aggacagctc cccacctgca
     9181 tcctctgaga gctcttccac cagagattct gccgtggcca tttctggcgc cgattcccga
     9241 ggaatcctag aagagccgtt gccttcaaca agcagtgaag aagaagatcc ccttgcgggt
     9301 atcagtctcc ctgaaggtgt ggacccctct tttctggctg ccctgcctga tgacatccgt
     9361 cgggaagttc tacagaacca gctaggcatt cgtccaccaa cccggactgc cccctccaca
     9421 aatagctcag cgcctgcagt ggtggggaat cctggtgtga ctgaggtgag tcccgagttt
     9481 cttgcggcac tgccacccgc gatccaagag gaagtactgg cacagcagag agctgagcag
     9541 cagcgacgag aactagcaca gaatgccagc tcagacaccc ctatggaccc tgtgaccttc
     9601 atccagactc tgccctcaga cctgcgccgt agtgtcctag aggatatgga ggacagtgtg
     9661 ttagctgtga tgccacctga cattgcagct gaggctcaag ccctgagacg agagcaagaa
     9721 gcccggcagc gacagctcat gcatgagcgt ctgtttgggc acagtagcac ctccgcactc
     9781 tctgctattc tccgaagccc ggctttcacc agtcgcttaa gtggcaaccg tggggtccag
     9841 tatactcgcc ttgctgtgca gagaggtggc accttccaga tggggggtag cagcagccat
     9901 aacaggcctt ctggcagtaa tgtagatact ctcctccgcc tccgaggacg gctccttctg
     9961 gaccacgaag ccctttcttg tctcttggtc ctactttttg tggatgagcc aaagctcaat
    10021 actagccgtc tacaccgagt actgagaaat ctctgctacc atgcccagac ccgccactgg
    10081 gtcatccgca gtctgctctc catcttgcag cgcagcagtg agagtgagct atgcattgaa
    10141 acacccaaac tcactacaag tgaggaaaag ggcaaaaagt cgagcaagag ctgtgggtca
    10201 agtagccatg agaaccgtcc cctggacctg ctacacaaga tggagtcaaa gagctccaac
    10261 cagctttcct ggctctcagt atccatggat gcagccctag gctgcaggac taatatattt
    10321 cagatccagc gttcaggggg gcgtaaacat accgagaagc atgcaagcgg tggctccacc
    10381 gtccacatcc atccccaagc tgctcctgtt gtctgcagac acgttttgga tacactcatt
    10441 caattggcca aggtatttcc cagccacttc acacagcagc ggaccaaaga aacaaactgt
    10501 gagagtgatc gggaaagggg caataaggcc tgtagcccat gctcctcaca gtcctccagc
    10561 agtggcattt gcacagactt ctgggactta ttggtaaaac tggacaacat gaatgtcagc
    10621 cggaaaggca agaactccgt gaagtcagtg ccagtgagcg ctggcggtga gggggaaacc
    10681 tctccataca gcctcgaggc ctctccactg gggcagctca tgaacatgtt gtcacaccca
    10741 gtcatccgcc ggagctctct cttaactgag aaactcctca gactcctttc tctcatctca
    10801 attgctctcc cagaaaacaa ggtgtcagaa gcacaggcta attctggcag cggtgcttcc
    10861 tccaccacca ctgccacctc aaccacatct accaccacca ccactgccgc ctccaccacg
    10921 cccacacccc ctactgcacc cacccctgtc acttctgctc cagccctggt tgctgccacg
    10981 gctatttcca ccattgtcgt agctgcttcg accacagtga ctacccccac gactgctacc
    11041 actactgttt caatttctcc cactactaag ggcagcaaat ctccagcgaa ggtgagtgat
    11101 gggggcagca gcagtacaga ctttaagatg gtgtcctctg gcctcactga aaaccagcta
    11161 cagctctctg tagaggtgtt gacatcccac tcttgttctg aggaaggctt agaggatgca
    11221 gccaacgtac tactgcagct ctcccggggg gactctggga cccgggacac tgttctcaag
    11281 ctgctactga atggagcccg ccatctgggt tatacccttt gtaaacaaat aggtaccctg
    11341 ctggccgagc tgcgggaata caacctcgag cagcagcggc gagcccaatg tgaaaccctc
    11401 tctcctgatg gcctgcctga ggagcagcca cagaccacca agctgaaggg caaaatgcag
    11461 agcaggtttg acatggctga gaatgtggta attgtggcat ctcagaagcg acctttgggt
    11521 ggccgggagc tccagctgcc ttctatgtcc atgttgacat ccaagacatc tacccagaag
    11581 ttcttcttga gggtactaca ggtcatcatc cagctccggg acgacacgcg ccgggctaac
    11641 aagaaagcca agcagacagg caggctaggt tcctccggtt taggctcagc tagcagcatc
    11701 caggcagctg ttcggcagct ggaggctgag gctgatgcca ttatacaaat ggtacgtgag
    11761 ggtcaaaggg cgcggagaca gcaacaagca gcaacgtcgg agtctagcca gtcagaggcg
    11821 tctgtccgga gggaggaatc acccatggat gtggaccagc catctcccag tgctcaagat
    11881 actcaatcca ttgcctccga tggaacccca cagggggaga aggaaaagga agaaagacca
    11941 cctgagttac ccctgctcag cgagcagctg agtttggacg agctgtggga catgcttggg
    12001 gagtgtctaa aggaactaga ggaatcccat gaccagcatg cggtgctagt gctacagcct
    12061 gctgtcgagg ccttctttct ggtccatgcc acagagcggg agagcaagcc tcctgtccga
    12121 gacacccgtg agagccagct ggcacacatc aaggacgagc ctcctccact ctcccctgcc
    12181 cccttaaccc cagccacgcc ttcctccctt gacccattct tctcccggga gccctcatct
    12241 atgcacatct cctcaagcct gccccctgac acacagaagt tccttcgctt tgcagagact
    12301 caccgcactg tgttaaacca gatcctacgg cagtccacga cccaccttgc tgatgggcct
    12361 tttgctgtcc tggtagacta cattcgtgtc ctcgactttg atgtcaagcg caaatatttc
    12421 cgccaagagc tggagcgttt agatgagggg ctccggaaag aagacatggc tgtgcatgtc
    12481 cgtcgtgacc atgtgtttga agactcctat cgtgagctgc atcgcaaatc ccccgaagaa
    12541 atgaagaatc gattgtatat agtatttgaa ggagaagaag ggcaggatgc tggcgggctc
    12601 ctgcgggagt ggtatatgat catctctcga gagatgttta accctatgta tgccttgttc
    12661 cgtacctcac ctggtgatcg agtcacctac accatcaatc catcttccca ctgcaacccc
    12721 aaccatctgt cctattttaa atttgtcgga cgcattgtgg ccaaagctgt atatgacaac
    12781 cgtcttctgg agtgctactt tactcgatcc ttttacaaac acatcttggg caagtcagtc
    12841 agatatacag atatggagag tgaagattac cacttctacc aaggtctggt ttatctgctg
    12901 gaaaatgatg tctccacact aggctatgac ctcaccttca gcactgaggt ccaagagttt
    12961 ggagtttgtg aagttcgtga cctcaaaccc aatggggcca acatcttggt aacagaggag
    13021 aataagaagg agtatgtaca cctggtatgc cagatgagaa tgacaggagc catccgcaag
    13081 cagttggcgg ctttcttaga aggcttctat gagatcattc caaagcgcct catttccatc
    13141 ttcactgagc aggagttaga gctgcttata tcaggactgc ccaccattga catcgatgat
    13201 ctgaaatcca acactgaata ccacaagtac cagtccaact ctattcagat ccagtggttc
    13261 tggagagcat tgcgttcttt cgatcaagct gaccgtgcca agttcctcca gtttgtcacg
    13321 ggtacttcca aggtacccct gcaaggcttt gctgccctcg aaggcatgaa tggcattcag
    13381 aagtttcaga tccatcgaga tgataggtcc acagatcgcc tgccttcagc tcacacatgt
    13441 tttaatcagc tggatctgcc tgcctatgag agctttgaga agctccgcca catgctactg
    13501 ttggctatcc aggagtgctc tgaaggcttt gggctggcct aataagcggc cgcgacccag
    13561 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
    13621 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
    13681 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
    13741 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
    13801 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
    13861 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
    13921 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
    13981 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
    14041 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
    14101 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtc cctgcgccgg
    14161 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
    14221 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
    14281 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
    14341 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
    14401 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
    14461 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
    14521 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
    14581 ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga
    14641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    14701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    14761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    14821 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    14881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    14941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    15001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    15061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    15121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    15181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    15241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    15301 ttttgctggc cttttgctca catgtt
//
