LOCUS       sequence-369247-        2223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pZ3 as a Type 2 part to be used in the Dueber YTK and Ellis 
            MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT001
FEATURES             Location/Qualifiers
     source          1..2223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     527..550
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      complement(590..624)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(695..1354)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1269..1288
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1536..2124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2025..2044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag
       61 taatacgctt aactgctcat tgctatattg aagtacggcc gcgtgggcgt gcgtgggcgg
      121 gcgtgggcgt gcgtgggcgg gcgtgggcgt gcgtgggcgt gtagaccgtg cgtcctcgtc
      181 atcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa
      241 agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc
      301 cacaaacctt caaattaacg aatcaaatta acaaccatag gatgataatg cgattagttt
      361 tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga
      421 tatataaatg gaaaagctgc ataaccactt taactaatac tttcaacatt ttcagtttgt
      481 attacttctt attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata
      541 ctttaacgtc aaggagaaaa aactataaga tctatgtgag accagaccaa taaaaaacgc
      601 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      661 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      721 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      781 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
      841 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
      901 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
      961 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1021 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1081 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1141 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1201 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1261 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1321 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1381 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1441 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     1501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgt
//
