LOCUS       sequence-369253-        1872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pLacFEC as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT005
FEATURES             Location/Qualifiers
     source          1..1872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    116..132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(239..273)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(344..1003)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     918..937
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1185..1773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1674..1693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacggcaca tgagcttggc gcgcccctcc ttgaaactga aattttagca
       61 tgtgattaat taaagattaa cttattttgt aattgtacta aaatataaaa ggaaattgtg
      121 agcggataac aactagcact gttgggcgag agtggaggcg ccggacaatt gtgagcggat
      181 aacaaaataa acacacataa acaaacaaag aaaaaaagat ctatgtgaga ccagaccaat
      241 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      301 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      361 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      421 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      481 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      541 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
      601 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      661 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
      721 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
      781 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
      841 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
      901 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
      961 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     1021 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1081 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     1141 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1201 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1261 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1321 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1381 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1441 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1501 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1561 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1621 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1681 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1741 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1801 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1861 gtggataacc gt
//
