LOCUS       sequence-369257-        8444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Glutamate sensor for localization to membrane contatct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, 
            Ide R, Hotta K, Oka K
  TITLE     Genetically encoded sensors for analysing neurotransmission among 
            synaptically-connected neurons
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2619..2638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2619..2637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2673..2682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2733..3662
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="ARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGA
                     FFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHR
                     HDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVML
                     YRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRI
                     VPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEP
                     RAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             3669..3710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3720..3776
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5024..5045
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(5382..5393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5791..5808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5797..6021
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6067..6495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6154..6173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6364..6385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6504..6524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6509..6838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6689..6824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6751..6770
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6905..7699
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6959..6978)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7569..7588
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7873..7994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7910..7929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7964..7983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8043..8059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8043..8059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8056..8078)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8067..8083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8091..8097,8098..8115,8116..8121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8136..8157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8274..8291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     2641 acccaagctg gctagcgttt aaacttaagc ttgccaccat gggctgtgtg cagtgtaagg
     2701 acaaggaggc taccaagctg acaggaggct ctgcgcgtaa ggtcgatctc acctcctgcg
     2761 atcgcgagcc gatccacatc cccggcagca ttcagccgtg cggctgcctg ctagcctgcg
     2821 acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc cggcgcgttc tttggacgcg
     2881 aaactccgcg ggtcggtgag ctactcgccg attacttcgg cgagaccgaa gcccatgcgc
     2941 tgcgcaacgc actggcgcag tcctccgatc caaagcgacc ggcgctgatc ttcggttggc
     3001 gcgacggcct gaccggccgc accttcgaca tctcactgca tcgccatgac ggtacatcga
     3061 tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa tccgctgcgg ctgacgcggc
     3121 agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga gatggccgca cgggtgccgc
     3181 gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt gtaccgcttc gcggacgacg
     3241 gctccgggat ggtgatcggc gaggcgaagc gcagcgacct cgagagcttt ctcggtcagc
     3301 actttccggc gtcgctggtc ccgcagcagg cgcggctact gtacttgaag aacgcgatcc
     3361 gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt gcccgagcac gacgcctccg
     3421 gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat ctcgccctgc catctcgaat
     3481 ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc gatcatcatt gacggcacgc
     3541 tatggggatt gatcatctgt catcattacg agccgcgtgc cgtgccgatg gcgcagcgcg
     3601 tcgcggccga aatgttcgcc gacttcttat cgctgcactt caccgccgcc caccaccaac
     3661 gcggatccgg caagcctatc cctaatcctc tgctgggact ggactctaca ggaagcggag
     3721 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctgaga
     3781 ccgacaccct cctgctatgg gtgctgctgc tctgggtgcc cggcagcacc ggcgacgcac
     3841 cggtcggagg caacgaccct aacctgttcg tcgccctgta cgacttcgtc gcctctggcg
     3901 acaacaccct gagcatcacc aagggcgaga agctgagagt cctgggctac aaccacaacg
     3961 gcgagtggtg cgaggcccag accaagaacg gccagggctg ggtccctagc aactacatca
     4021 cacctgtcaa cagcaccggt ggaggcagcg gaggaggctc cggagccgca ggcagcacgc
     4081 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtgcctt
     4141 tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
     4201 ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
     4261 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tttcgatttt gaatgtggtt
     4321 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
     4381 tcggtacgcg cctgttgacc aaaaagggtg gcgatatcaa agattttgcc aacctgaaag
     4441 acaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
     4501 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
     4561 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgtgctgctg gccggtgaac
     4621 gtgcgaaagc gaagaaacca gacaactggg aaatcgtcgg caagccgcag tctcaggagg
     4681 cctacggttg tatgttgcgt aaagatgatc cgcagttcaa aaagctgatg gatgacacca
     4741 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataagtgg ttcaaaaatc
     4801 caattctggt gagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     4861 cgaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc gaccactacc
     4921 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     4981 atcagtccgt gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5041 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga ggaggctccg
     5101 gaggcggaag cggtaccctc gagctggtgc cccacttgca taacctcaat gacatcagcc
     5161 aatacacaag tacaaccacc aaggtcccaa gcactgacat cacccttcga ccaactcgga
     5221 aaaactcaac ccctgtgacc agcgcatttc caaccgctaa gcaagacgac cccaaacaac
     5281 agcctagtcc atttagcgta gaccaaaggg actacagcac agagctgtcc gtgaccatag
     5341 ctgtaggagc ttccctgctt ttcctgaaca ttctggcttt cgcggccctc tattacaaga
     5401 aggacaaaag gcgccacgac gtacatagga gatgtagtcc gcaacgaact acaaccaatg
     5461 atcttacgca cgcacctgag gaggagatca tgagtcttca gatgaaacac acagacctgg
     5521 accacgagtg tgaatccatc catccccatg aggtggtgct gaggacagca tgccctccgg
     5581 actataccct ggcaatgcgc cgaagcccag acgacattcc cctgatgaca cccaatacca
     5641 taaccatgat tcccaacact attccaggaa tccagcctct ccacactttc aacacattca
     5701 ctggtggaca gaataatact ctccctcatc cgcatccaca cccccactcc cacagctaat
     5761 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5821 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5881 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5941 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6001 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6061 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6481 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6841 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     6901 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6961 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7021 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7081 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7141 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7201 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7261 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7321 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7381 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     7441 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7501 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7561 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7621 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7681 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     7741 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7801 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7861 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7921 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7981 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8041 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8101 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8161 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtt
//
