LOCUS       sequence-36926-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Kitazono AA
  TITLE     Optimized protocols and plasmids for in vivo cloning in yeast.
  JOURNAL   Gene. 2011 Sep 15;484(1-2):86-9. doi: 10.1016/j.gene.2011.06.006. 
            Epub 2011 Jun 22.
  PUBMED    21722715
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-09-15"; volume: "484(1-2):86-9. doi"; pages: " 
            10.1016/j.gene.2011.06.006. Epub 2011 Jun 22"
COMMENT     Sequence Label: pRS316-IVb
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..64
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     49..65
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(216..235)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(217..235)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(242..259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(242..258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(251..273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      399..854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(486..505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     696..717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gactgaatac cctactaaag ggacaaaagc tggtaccggg ccccccctcg aggtcgacgg
       61 tatcgataag cttgatatcg aattcctgca ggccttcgcg agacgtccat ggccggctag
      121 catgcatatg tttaaactac gtagcgcttg agatctgcag cccgggggat ccactagttc
      181 tagagcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg tattacaatt
      241 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      301 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      361 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
      421 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      481 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
      541 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      601 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      661 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      721 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      781 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
      841 aacgcttaca atttcctgat gcggtatttt nctccttacg catctgtgcg gtatttcaca
      901 ccgcanaggg
//
