LOCUS       sequence-369262-        8426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Glutamate sensor for localization to membrane contatct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, 
            Ide R, Hotta K, Oka K
  TITLE     Genetically encoded sensors for analysing neurotransmission among 
            synaptically-connected neurons
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1
FEATURES             Location/Qualifiers
     source          1..8426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2619..2638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2619..2637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2673..2682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2733..3662
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="ARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGA
                     FFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHR
                     HDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVML
                     YRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRI
                     VPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEP
                     RAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             3669..3710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3720..3776
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5364..5375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5773..5790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5779..6003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6049..6477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6136..6155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6346..6367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6486..6506)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6491..6820
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6671..6806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6733..6752
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6887..7681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6941..6960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7551..7570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7855..7976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7892..7911)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7946..7965
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8025..8041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8025..8041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8038..8060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8049..8065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8073..8079,8080..8097,8098..8103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8118..8139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8256..8273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     2581 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     2641 acccaagctg gctagcgttt aaacttaagc ttgccaccat gggctgtgtg cagtgtaagg
     2701 acaaggaggc taccaagctg acaggaggct ctgcgcgtaa ggtcgatctc acctcctgcg
     2761 atcgcgagcc gatccacatc cccggcagca ttcagccgtg cggctgcctg ctagcctgcg
     2821 acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc cggcgcgttc tttggacgcg
     2881 aaactccgcg ggtcggtgag ctactcgccg attacttcgg cgagaccgaa gcccatgcgc
     2941 tgcgcaacgc actggcgcag tcctccgatc caaagcgacc ggcgctgatc ttcggttggc
     3001 gcgacggcct gaccggccgc accttcgaca tctcactgca tcgccatgac ggtacatcga
     3061 tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa tccgctgcgg ctgacgcggc
     3121 agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga gatggccgca cgggtgccgc
     3181 gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt gtaccgcttc gcggacgacg
     3241 gctccgggat ggtgatcggc gaggcgaagc gcagcgacct cgagagcttt ctcggtcagc
     3301 actttccggc gtcgctggtc ccgcagcagg cgcggctact gtacttgaag aacgcgatcc
     3361 gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt gcccgagcac gacgcctccg
     3421 gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat ctcgccctgc catctcgaat
     3481 ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc gatcatcatt gacggcacgc
     3541 tatggggatt gatcatctgt catcattacg agccgcgtgc cgtgccgatg gcgcagcgcg
     3601 tcgcggccga aatgttcgcc gacttcttat cgctgcactt caccgccgcc caccaccaac
     3661 gcggatccgg caagcctatc cctaatcctc tgctgggact ggactctaca ggaagcggag
     3721 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctgaga
     3781 ccgacaccct cctgctatgg gtgctgctgc tctgggtgcc cggcagcacc ggcgacgcac
     3841 cggtcggagg caacgaccct aacctgttcg tcgccctgta cgacttcgtc gcctctggcg
     3901 acaacaccct gagcatcacc aagggcgaga agctgagagt cctgggctac aaccacaacg
     3961 gcgagtggtg cgaggcccag accaagaacg gccagggctg ggtccctagc aactacatca
     4021 cacctgtcaa cagcaccggt ggaggcagcg gaggaggctc cggagccgca ggcagtacgc
     4081 tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct tcagtgcctt
     4141 tctcttttta cgacaatcag caaaaagtgg tgggttactc gcaggattac tccaacgcca
     4201 ttgttgaagc agtgaagaag aaactcaaca aaccggactt gcaggtaaaa ctgattccga
     4261 ttacctcaca aaaccgtatt ccactgctgc aaaacggcac tatcgatttt gaatgtggtt
     4321 ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact attttcgtgg
     4381 tcggtacgcg cctgttgacc aaaaagggtg gcgatatcaa agattttgcc aacctgaaag
     4441 acaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac aaactgaatg
     4501 aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac tctttccgca
     4561 ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgtgctgctg gccggtgaac
     4621 gtgcgaaagc gaagaaacca gacaactggg aaatcgtcgg caagccgcag tctctggagg
     4681 cctacggttg tatgttgcgt aaagatgatc cgcagttcaa aaagctggtg gatgacacca
     4741 tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataagtgg ttcaaaaatc
     4801 caattgaggt ggtttccgag cggatgtacc ccgaggacgg cgccctgaag agcgtgatca
     4861 ggaaggggct gaggctgaag gacggcggcc actacgccgc cgaggccaag accacctaca
     4921 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt ggatatcaag ttggacatcg
     4981 tgtcccacaa cgaggactac accatcgtgg aacagtgcga acgcgccgag ggccgccact
     5041 ccaccggcgg catggacgag ctgtacaagg gaggaggctc cggaggcgga agcggtaccc
     5101 tcgagctggt gccccacttg cataacctca atgacatcag ccaatacaca agtacaacca
     5161 ccaaggtccc aagcactgac atcacccttc gaccaactcg gaaaaactca acccctgtga
     5221 ccagcgcatt tccaaccgct aagcaagacg accccaaaca acagcctagt ccatttagcg
     5281 tagaccaaag ggactacagc acagagctgt ccgtgaccat agctgtagga gcttccctgc
     5341 ttttcctgaa cattctggct ttcgcggccc tctattacaa gaaggacaaa aggcgccacg
     5401 acgtacatag gagatgtagt ccgcaacgaa ctacaaccaa tgatcttacg cacgcacctg
     5461 aggaggagat catgagtctt cagatgaaac acacagacct ggaccacgag tgtgaatcca
     5521 tccatcccca tgaggtggtg ctgaggacag catgccctcc ggactatacc ctggcaatgc
     5581 gccgaagccc agacgacatt cccctgatga cacccaatac cataaccatg attcccaaca
     5641 ctattccagg aatccagcct ctccacactt tcaacacatt cactggtgga cagaataata
     5701 ctctccctca tccgcatcca cacccccact cccacagcta atctagaggg cccgtttaaa
     5761 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5821 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5881 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5941 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6001 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6181 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6481 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6541 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6661 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6721 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6781 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6841 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7381 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7681 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7741 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7801 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7861 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7921 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7981 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8401 cgtaaaaagg ccgcgttgct ggcgtt
//
