LOCUS       sequence-369263-        2212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pLacFBA1 as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT007
FEATURES             Location/Qualifiers
     source          1..2212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    456..472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    509..525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(579..613)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(684..1343)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1258..1277
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1525..2113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2014..2033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgatgac agcaggatta tcgtaatacg taatagttga aaatctcaaa
       61 aatgtgtggg tcattacgta aataatgata ggaatgggat tcttctattt ttcctttttc
      121 cattctagca gccgtcggga aaacgtggca tcctctcttt cgggctcaat tggagtcacg
      181 ctgccgtgag catcctctct ttccatatct aacaactgag cacgtaacca atggaaaagc
      241 atgagcttag cgttgctcca aaaaagtatt ggatggttaa taccatttgt ctgttctctt
      301 ctgactttga ctcctcaaaa aaaaaaaatc tacaatcaac agatcgcttc aattacgccc
      361 tcacaaaaac ttttttcctt cttcttcgcc cacgttaaat tttatccctc atgttgtcta
      421 acggatttct gcacttgatt tattataaaa gacaattgtg agcggataac aaaaagcact
      481 gttgggggtg agtggaggcg ccgggtaatt gtgagcggat aacaacataa acacacataa
      541 acaaacaaaa taaaaaagat ctatgtgaga ccagaccaat aaaaaacgcc cggcggcaac
      601 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      661 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
      721 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      781 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
      841 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
      901 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
      961 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1021 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1081 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     1141 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1201 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1261 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1321 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     1381 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     1441 ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2161 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gt
//
