LOCUS       sequence-369266-        2190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pLacENO2 as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT008
FEATURES             Location/Qualifiers
     source          1..2190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    435..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    488..504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(557..591)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(662..1321)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1236..1255
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1503..2091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1992..2011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacggtgtc gacgctgcgg gtatagaaag ggttctttac tctatagtac
       61 ctcctcgctc agcatctgct tcttcccaaa gatgaacgcg gcgttatgtc actaacgacg
      121 tgcaccaact tgcggaaagt ggaatcccgt tccaaaactg gcatccacta attgatacat
      181 ctacacaccg cacgcctttt ttctgaagcc cactttcgtg gactttgcca tatgcaaaat
      241 tcatgaagtg tgataccaag tcagcataca cctcactagg gtagtttctt tggttgtatt
      301 gatcatttgg ttcatcgtgg ttcattaatt ttttttctcc attgctttct ggctttgatc
      361 ttactatcat ttggattttt gtcgaaggtt gtagaattgt atgtgacaag tggcaccaag
      421 catataaaag tgaattgtga gcggataaca agtagcactg ttggccgtga gtggaggcgc
      481 cggcaaattg tgagcggata acaaattaaa cacacataaa caaacaaata aaaaagatct
      541 atgtgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      601 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
      661 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      721 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      781 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      841 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
      901 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      961 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1021 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1081 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1141 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1201 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1261 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1321 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1381 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
     1441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2161 gcgttatccc ctgattctgt ggataaccgt
//
