LOCUS       sequence-369267-        2111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pLacSpTDH3 as a Type 2 part to be used in the Dueber YTK and
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT009
FEATURES             Location/Qualifiers
     source          1..2111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    356..372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    409..425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(478..512)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(583..1242)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1157..1176
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1424..2012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1913..1932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacggtttt atttctgctg ccatccgtaa atgccaggat ttgagcgggt
       61 tacacaatat atctcatatt ttcggtgtct gggtcattac tttactcttg gcatccacta
      121 aatatattgg agcctgcttt ttaaactggc ttccagaaaa aaatcaatgg agtgatgcaa
      181 actgcctgga gtaaaagatg acacaaggcg attgacctac gcatgtatct atctgatttt
      241 cttacacctt ctatttcatt ctaactcttt gatttggaaa acacctaaga aaaaaaaggt
      301 tgaaatcagt tccctgaaat tgtcccccta cttgactaat aaatataaaa ggtaattgtg
      361 agcggataac aacaagcact gttgggcgtg agtggaggcg ccggataatt gtgagcggat
      421 aacaatttaa acacacataa acaaacaaaa aaaaaagatc tatgtgagac cagaccaata
      481 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
      541 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
      601 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
      661 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
      721 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
      781 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
      841 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
      901 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
      961 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1021 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1081 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1141 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1201 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1261 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1321 gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt
     1381 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1561 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2041 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2101 tggataaccg t
//
