LOCUS       sequence-369268-        1887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pTet2 as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT010
FEATURES             Location/Qualifiers
     source          1..1887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(254..288)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(359..1018)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     933..952
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1200..1788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1689..1708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgagaat ctatcggtcc ctatcagtga tagagaatct gctgatcgtc
       61 cctatcagtg atagagaatc aaatcagtta ataagtactt aatttaatat aaagaggtgt
      121 cgttcttcgt tgtgttgata cagtacgtac atcgatgagg acactaaaaa acaaacaaaa
      181 aaaaagaaaa tactacatac attttgcttt tagcactaac atacctaaaa aagatctatg
      241 tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
      301 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
      361 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      421 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      481 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      541 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
      601 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
      661 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
      721 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
      781 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
      841 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
      901 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
      961 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1021 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1081 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct
     1141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1501 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1561 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1621 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1681 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1741 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1801 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1861 ttatcccctg attctgtgga taaccgt
//
