LOCUS       sequence-36930-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kitazono AA
  TITLE     Optimized protocols and plasmids for in vivo cloning in yeast.
  JOURNAL   Gene. 2011 Sep 15;484(1-2):86-9. doi: 10.1016/j.gene.2011.06.006. 
            Epub 2011 Jun 22.
  PUBMED    21722715
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2011-09-15"; volume: "484(1-2):86-9. doi"; pages: " 
            10.1016/j.gene.2011.06.006. Epub 2011 Jun 22"
COMMENT     Sequence Label: pRS316-IVa
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(215..234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(216..234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(241..258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(241..257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(250..272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      398..853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(485..504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     695..716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gactgattac ctactaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcctgcag atctcaagcg ctacgtagtt taaacatatg
      121 catgctagcc ggccatggac gtctcgcgaa ggcctgcagc ccgggggatc cactagttct
      181 agagcggccg ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc
      241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      361 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
      421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      841 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      901 gcanagggta ataactgana taattaaatt gaagctctaa attggagtag
//
