LOCUS       sequence-369307-       11237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-sfGFP10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H
  TITLE     Background-suppressed live visualization of genomic loci with an 
            improved CRISPR system based on a split fluorophore.
  JOURNAL   Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. 
            Epub 2020 Sep 4.
  PUBMED    32887690
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.260018.119"; journalName: "Genome Res"; date: "2020-09";
            volume: "30"; issue: "9"; pages: "1306-1316"
COMMENT     Sequence Label: pM159
FEATURES             Location/Qualifiers
     source          1..11237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2299..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2757..2777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2808..2826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2857..2881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2959..2979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2980..3003,3004..3006,3007..5493,5494..5496,
                     5497..7080)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7090..7110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7129..7149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7378..7539
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             7567..7587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7627..8215
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7680..7700)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8098..8109)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8290..8523
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8642..8660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8728..8832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8833..8901,8902..9693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9051..9070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9864..10452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10353..10372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10606..10623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10863..10884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10899..10904,10905..10922,10923..10929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10937..10953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10942..10964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10961..10977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10961..10977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11027..11046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
     2221 gctagctgat gcgggcccta gttattaata gtaatcaatt acggggtcat tagttcatag
     2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
     2821 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2941 cctaccggta tggctagccc caaaaagaag aggaaagtgg acaagaagta ttctatcgga
     3001 ctggccatcg ggactaatag cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc
     3061 tctaagaagt tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa aaatctgatc
     3121 ggagctctcc tctttgattc aggggagacc gctgaagcaa cccgcctcaa gcggactgct
     3181 agacggcggt acaccaggag gaagaaccgg atttgttacc ttcaagagat attctccaac
     3241 gaaatggcaa aggtcgacga cagcttcttc cataggctgg aagaatcatt cctcgtggaa
     3301 gaggataaga agcatgaacg gcatcccatc ttcggtaata tcgtcgacga ggtggcctat
     3361 cacgagaaat acccaaccat ctaccatctt cgcaaaaagc tggtggactc aaccgacaag
     3421 gcagacctcc ggcttatcta cctggccctg gcccacatga tcaagttcag aggccacttc
     3481 ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt catccagctg
     3541 gtgcagactt acaaccagct ctttgaagag aaccccatca atgcaagcgg agtcgatgcc
     3601 aaggccattc tgtcagcccg gctgtcaaag agccgcagac ttgagaatct tatcgctcag
     3661 ctgccgggtg aaaagaaaaa tggactgttc gggaacctga ttgctctttc acttgggctg
     3721 actcccaatt tcaagtctaa tttcgacctg gcagaggatg ccaagctgca actgtccaag
     3781 gacacctatg atgacgatct cgacaacctc ctggcccaga tcggtgacca atacgccgac
     3841 cttttccttg ctgctaagaa tctttctgac gccatcctgc tgtctgacat tctccgcgtg
     3901 aacactgaaa tcaccaaggc ccctctttca gcttcaatga ttaagcggta tgatgagcac
     3961 caccaggacc tgaccctgct taaggcactc gtccggcagc agcttccgga gaagtacaag
     4021 gaaatcttct ttgaccagtc aaagaatgga tacgccggct acatcgacgg aggtgcctcc
     4081 caagaggaat tttataagtt tatcaaacct atccttgaga agatggacgg caccgaagag
     4141 ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc agcgcacttt cgacaatggg
     4201 agcattcccc accagatcca tcttggggag cttcacgcca tccttcggcg ccaagaggac
     4261 ttctacccct ttcttaagga caacagggag aagattgaga aaattctcac tttccgcatc
     4321 ccctactacg tgggacccct cgccagagga aatagccggt ttgcttggat gaccagaaag
     4381 tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg tggacaaggg agccagcgct
     4441 cagtcattca tcgaacggat gactaacttc gataagaacc tccccaatga gaaggtcctg
     4501 ccgaaacatt ccctgctcta cgagtacttt accgtgtaca acgagctgac caaggtgaaa
     4561 tatgtcaccg aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt
     4621 gtggaccttc tgttcaagac caatagaaag gtgaccgtga agcagctgaa ggaggactat
     4681 ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg gggtcgaaga tcggttcaac
     4741 gcaagcctgg gtacctacca tgatctgctt aagatcatca aggacaagga ttttctggac
     4801 aatgaggaga acgaggacat ccttgaggac attgtcctga ctctcactct gttcgaggac
     4861 cgggaaatga tcgaggagag gcttaagacc tacgcccatc tgttcgacga taaagtgatg
     4921 aagcaactta aacggagaag atataccgga tggggacgcc ttagccgcaa actcatcaac
     4981 ggaatccggg acaaacagag cggaaagacc attcttgatt tccttaagag cgacggattc
     5041 gctaatcgca acttcatgca acttatccat gatgattccc tgacctttaa ggaggacatc
     5101 cagaaggccc aagtgtctgg acaaggtgac tcactgcacg agcatatcgc aaatctggct
     5161 ggttcacccg ctattaagaa gggtattctc cagaccgtga aagtcgtgga cgagctggtc
     5221 aaggtgatgg gtcgccataa accagagaac attgtcatcg agatggccag ggaaaaccag
     5281 actacccaga agggacagaa gaacagcagg gagcggatga aaagaattga ggaagggatt
     5341 aaggagctcg ggtcacagat ccttaaagag cacccggtgg aaaacaccca gcttcagaat
     5401 gagaagctct atctgtacta ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt
     5461 gatatcaaca ggctctcaga ctacgacgtg gacgccatcg tccctcagag cttcctcaaa
     5521 gacgactcaa ttgacaataa ggtgctgact cgctcagaca agaaccgggg aaagtcagat
     5581 aacgtgccct cagaggaagt cgtgaaaaag atgaagaact attggcgcca gcttctgaac
     5641 gcaaagctga tcactcagcg gaagttcgac aatctcacta aggctgagag gggcggactg
     5701 agcgaactgg acaaagcagg attcattaaa cggcaacttg tggagactcg gcagattact
     5761 aaacatgtcg cccaaatcct tgactcacgc atgaatacca agtacgacga aaacgacaaa
     5821 cttatccgcg aggtgaaggt gattaccctg aagtccaagc tggtcagcga tttcagaaag
     5881 gactttcaat tctacaaagt gcgggagatc aataactatc atcatgctca tgacgcatat
     5941 ctgaatgccg tggtgggaac cgccctgatc aagaagtacc caaagctgga aagcgagttc
     6001 gtgtacggag actacaaggt ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag
     6061 atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt cttcaagacc
     6121 gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc tcatcgagac taatggggag
     6181 actggcgaaa tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa agtgctttct
     6241 atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa
     6301 tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga ttgggaccct
     6361 aagaagtacg gcggattcga ttcaccaact gtggcttatt ctgtcctggt cgtggctaag
     6421 gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg aactgctggg tatcaccatt
     6481 atggagcgca gctccttcga gaagaaccca attgactttc tcgaagccaa aggttacaag
     6541 gaagtcaaga aggaccttat catcaagctc ccaaagtata gcctgttcga actggagaat
     6601 gggcggaagc ggatgctcgc ctccgctggc gaacttcaga agggtaatga gctggctctc
     6661 ccctccaagt acgtgaattt cctctacctt gcaagccatt acgagaagct gaaggggagc
     6721 cccgaggaca acgagcaaaa gcaactgttt gtggagcagc ataagcatta tctggacgag
     6781 atcattgagc agatttccga gttttctaaa cgcgtcattc tcgctgatgc caacctcgat
     6841 aaagtcctta gcgcatacaa taagcacaga gacaaaccaa ttcgggagca ggctgagaat
     6901 atcatccacc tgttcaccct caccaatctt ggtgcccctg ccgcattcaa gtacttcgac
     6961 accaccatcg accggaaacg ctatacctcc accaaagaag tgctggacgc caccctcatc
     7021 caccagagca tcaccggact ttacgaaact cggattgacc tctcacagct cggaggggat
     7081 gagggagctc ccaagaaaaa gcgcaaggta ggtagttccc tcgagatgcc taagaaaaag
     7141 cgcaaggtgg gaggcggtgg gtctggggga ggcggtagtg gcggtggagg ctcaatggac
     7201 ctgcccgacg accactacct gtccacccag accatcctgt ccaaggacct gaacgacgtt
     7261 ggtggtggcg gatcagaagg aggcggtagc gggggccctg gttcgggagg ggaaggttct
     7321 gctgggggag ggagcgctgg cggggggtct atcgatggtg gaggtcggac cgaagagtac
     7381 aagcttatcc tgaacggtaa aaccctgaaa ggtgaaacca ccaccgaagc tgttgacgct
     7441 gctaccgcgg aaaaagtttt caaacagtac gctaacgaca acggtgttga cggtgaatgg
     7501 acctacgacg acgctaccaa aaccttcacg gtaaccgaag gtggtggtag cggtggtggt
     7561 actagtccca agaagaagcg caaggtgtaa gcggccgctc tggctctcga gtagggtaat
     7621 ctagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7681 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7741 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7801 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7861 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7921 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7981 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8041 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8101 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8161 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     8221 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8281 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8341 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8521 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8581 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     8641 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8701 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9061 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9781 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9841 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9901 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9961 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10021 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10081 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10201 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10261 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10321 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10381 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10441 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10501 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10561 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10621 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10681 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10741 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10801 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10861 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10921 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    10981 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11041 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    11101 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    11161 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11221 actacaaact tagtagt
//
