LOCUS       sequence-369314-        4231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sfGFP under light light responsive PEL222 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Pirhanov A, Bridges CM, Goodwin RA, Guo YS, Furrer J, Shor LM, Gage 
            DJ, Cho YK
  TITLE     Optogenetics in Sinorhizobium meliloti Enables Spatial Control of 
            Exopolysaccharide Production and Biofilm Structure.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):345-356. doi: 
            10.1021/acssynbio.0c00498. Epub 2021 Jan 19.
  PUBMED    33465305
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00498"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "345-356"
COMMENT     Sequence Label: pAP01
FEATURES             Location/Qualifiers
     source          1..4231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(76..162)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             294..1007
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1094..1188
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1303..2493
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1655..1674)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            2639..2747
                     /note="incP origin of transfer"
     CDS             complement(2758..3420)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGXVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3421..4192
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtaggggggg cgcgcccagc
       61 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      121 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgccttt aattaaagcg
      181 gataacaatt tcacacagag ccggtagcct ttagtccatg ttagcgaaga aaatggtttg
      241 ttatagtcga ataaacacac agaattcatt aagaaagaag gagatatggt accatgcgta
      301 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      361 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      421 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      481 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
      541 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
      601 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
      661 ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat
      721 acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag
      781 cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
      841 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca
      901 cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
      961 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaatga acacgggatc
     1021 ctctagagtc gacctgcagg catgcaagct tgcggccgcg tcgtgactgg gaaaaccctg
     1081 gcgactagtc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     1141 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc
     1201 aataattacg atttacgtat ttaaatttga cagcttatca tcgataaact gtaatgcggt
     1261 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     1321 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     1381 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     1441 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     1501 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     1561 tacgcgatca tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcc
     1621 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     1681 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     1741 ggccccgtgg ccgggggact gttgggcgcc atctccctgc acgcaccatt ccttgcggcg
     1801 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     1861 ggagagcgcc gtccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     1921 cggggcatga ccattgtggc cgcacttatg actgtcttct ttatcatgca actcgtagga
     1981 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     2041 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     2101 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     2161 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     2221 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     2281 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     2341 acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     2401 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     2461 cgcggtgcat ggagccgggc cacctcgacc tgagacaatt gtccttttcc gctgcataac
     2521 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     2581 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     2641 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     2701 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggcccta
     2761 ccggcgcggc agcgttaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca
     2821 cggctcatcg ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt
     2881 caaggctggc aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc
     2941 ggggccggtc ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc
     3001 atgccccaac agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac
     3061 cgacaggcgc aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc
     3121 cgacacgkkg ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc
     3181 cttgactgcg tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg
     3241 gctgaccacc acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc
     3301 cgccgtgggt ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg
     3361 cacccagtga ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat
     3421 gcttatctcc atgcggtagg ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa
     3481 cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt
     3541 tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt gcgtaccccc
     3601 cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta gttctttggt
     3661 gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct ccccctccgg
     3721 caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg
     3781 tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg gcaggcgggc
     3841 gggcttcgcc cttcgactgc ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag
     3901 gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc
     3961 ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat
     4021 tgatggggca aggccgcagg ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg
     4081 gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct
     4141 tgtccagcag ggttgtccac gggccgagcg aagcgagcca gccggtggcc gctcgcggcc
     4201 atcgtccaca tatccacggg ctggcaaggg a
//
