LOCUS       sequence-369317-        8410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light
            light responsive PEL222 promoter and EL222 under constitutively 
            active LacIq promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Pirhanov A, Bridges CM, Goodwin RA, Guo YS, Furrer J, Shor LM, Gage 
            DJ, Cho YK
  TITLE     Optogenetics in Sinorhizobium meliloti Enables Spatial Control of 
            Exopolysaccharide Production and Biofilm Structure.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):345-356. doi: 
            10.1021/acssynbio.0c00498. Epub 2021 Jan 19.
  PUBMED    33465305
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00498"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "345-356"
COMMENT     Sequence Label: pAP14
FEATURES             Location/Qualifiers
     source          1..8410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4370..4464
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     RBS             4611..4622
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5312..6502
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGRTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5664..5683)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            6648..6756
                     /note="incP origin of transfer"
     CDS             complement(6767..7429)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      7430..8201
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(8316..8402)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aattaaagcg aataacaatt tcacacagag ccggtagcct ttagtccatg ttagcgaaga
       61 aaatggtttg ttatagtcga ataaaggtac tagcgaacgt ttaagaagga gatatacata
      121 ataatatgtc ttctaacgtg aggcagtcgc cggcgaagcg aaggctggtc gtggcatccg
      181 gtctggaccg ggaaaatttc gaagggagcg acatcgagca tctggttcat tttctcgaac
      241 cggatgcagc tttgcgattt cccctgcata acgcctttcc tcttatcgcg cttgccttct
      301 cggatgaggg tgaagccgat ctgcgggaag ggctcgcgtc catcaatgct cttggtacca
      361 tccccgagat accgcttcag cggatcgacg cggacggtaa agcggaaagc cttgcgctcg
      421 tgcggtcgct ggtggaggga ggagtgggtc ggctctcccg tttcacgtcg tcggtaaccg
      481 ccgagctcgc cattctccgg cgtgagcgag aaacgctgct cgaaaattac cgcgcgctgg
      541 aggatgcctt tcaggcccgc aattgggaac cggtcgcgga gatattcgcc cacgaccctt
      601 atgccgaccc gaaggacgag ggcatcgggc agctccttgc gaccggctgc gtcgagcagc
      661 ttcttccggt ctctagtctt ggcgttgcgg gggtcgcgct gcaccttcat tccgtgccca
      721 gggacggcgg cgaactggtc gtcatgctgg gttatgtcga gaatggcgag ggcgtggccg
      781 aatggaccgt gccctatgcg cagctcgcgg caaactggaa tttcctcgcc ctgccgcgcg
      841 cttgcggcgg cgccgcgaga acgctgcggc ttagaatctc aacgaccggc acggagactg
      901 tagggctttc actcggctat ccgatcgcga gcgagcggta cacagcccgc tcggaaaccc
      961 cgcatcccga tctcgatctc cggcccctgg cgttccgcgt ctttacggga ctgcccggcg
     1021 tcaaaccgac gcgcatgccg aacatgatcg ctccgaccgc cttgctcgaa ggccacttca
     1081 tcgaggatta tcgtctggcg gtcgaattgc ttagtcagat cgtcgacgtt tcggtcacgc
     1141 cggtcgttcc cgaattccag accgtccgct tcctcgagca tgagaacgcg gtcgtttgcc
     1201 acccgctgcc gagtggaatc tccgccggca cgatcggccg cgcagtggaa cccggcacca
     1261 tctccttctc cgcaagcgcg atcatcgatc acccgaaggg cgcacccgcg gccgtgagct
     1321 tcctgctcgc tccggcgaac tccaatgcgc gctcggaagt ggcggaactc gcgcgcaagg
     1381 gtgctgccaa gccctcggcc ttcttcagcg gctggcgcga ggtcacctcc gagcaggcgg
     1441 tcaacgtgaa ctttcaactg gacaagcccg tgcgcgagcc gatggacctc atgattctca
     1501 gccgcgcggt gacggatacg gtcgatttct cctggctcaa ggtctccggc ttccgattgg
     1561 taaagcagtc cgcaggggcg tccaatgtcc ggaaataagg aaaactcgga cctgatcgtg
     1621 gtggcaatgc cgctttatgg ccacgcagcc ctcgtcctgg aggcgatcga gtccgtgctc
     1681 gcttcgaagc ttgccgggtg ccgggtggca gtggtggtgt ccgtcgatgg tgatccgcga
     1741 caggagacat tcgatcagtt gctgctctat gcggcggccc atcctgcggt tcatgtgctt
     1801 ttcggggcca atgccgggcc gggcggggcg cgcaatcgcg cgatcgacta cgtgctcgcg
     1861 aatctgccgg aagcagaggc cgtctatttt ctcgatgccg acaatcgcgt gctgcccggg
     1921 accatcgaga ctctctaccg gcaactgcgc gccagcggct gcggctggat ctataccaat
     1981 atcgacacgt tttcggtgag ctggcgcgcc cattacggca atcgctactc gcggcttctg
     2041 cactgcatca cggacaatat ctgcgacacc ggttcgatga tctcgatcga cgttttccgg
     2101 gcgggggtcc gcttcgacga cgacaggcag aacggtttcg aggattggga gttctggctg
     2161 tcctgcatcg agcacggctt cgtgggggcg ccgtgccacg acacgacctt cgaataccgg
     2221 ctgagggcgg aaagccgctt caaggaggcg aaccgcgatc gcgccgcctc ggtgagcttc
     2281 ctcagaaagc gccaccgggc gctgttccag cggccaatgc tggtcgattt cgagcacgag
     2341 gaatgcccgc gctatctttt cgcacggacg gaagatgccg ccatttcgtt ctttacggat
     2401 ccgaccaaga cgcccaggcg tcttcgcctc gacgatatca ttccagcctt ctgggcgagc
     2461 atcggcgagc ctgacaacgt tcacttcccg cccttcctga tcgccggaag cggtgccacg
     2521 ctcgatctgc tgctgcgctc ccggatggtg ccgaacgtgc tctcccatct ggagcgcctg
     2581 agcgagaagg ccaacgtggt cttcgtccag ctcggcaacg atgcggctca gcgcaagatc
     2641 gagccggtct tcctggaggc cggcgcccag cacaacgcac cggccgacct catcttcctg
     2701 tccacttcgc tcgtgcgcga cgtcattcat aacaaggcgc tggactggtt tgcctccatc
     2761 ggcaaccagc aggtctggcc gacatcggca attctgaaag tccgcttccc tttcccgaga
     2821 agcctgccgc gccgctcgct catcacgccc cagcaggtga tgatcaattg cgtcaatgcc
     2881 atcgcgacca gtccgctgcg gcggaccgcc ggcaaacgct ggacctggcg tccggcacgg
     2941 ctcgttccct attccgacct ccacaaggcg cttcgccacg agatcggtgg ctcgcccgtg
     3001 ttgccgctcg gtcatagcga gggaagcagg aagaccgcag ccctgcttgt tccgaatgca
     3061 tccttcggcg gtgccgagaa ggtcgtatat gcggcctcgc gcgagctgaa ggccgccggc
     3121 tacgagaccc atctcttcgt gctcggcaca tccaggatgg atgtgatcga cgagttcgat
     3181 cagagcttcg actatattca cttctgggac caaggcattc ccgcctgggg cggctccggc
     3241 tcgttcctgg ggcaggactt catcgccgag ggccacgacg tcgattgggc tgcgctcaag
     3301 ggtcagcttt ccggcttcga cctcgtgatc aacaatcacg tcatggcggt gcatccgctt
     3361 atcgcgcggt tacgctcgga gggaacgcgc accgcctgct accttcacgt ggtcgacaat
     3421 acctccttca aaaggcccgc cggtcagccc ttcgcggcga ttgcccacga gcattgctac
     3481 gatgccttcc tgacctgctc ggagcagctc aagatctatc tgcacagctt cggcgttccc
     3541 tatgaaaaga tcttcgccgt ccccaacggc gcgagcttct ccgtgccgcc caaggttctc
     3601 gcggaggtgc tgtcggtcag gcgcatcgaa cgcaaggacg atcggcttcg ggtcctctat
     3661 atgggtcggc tcgatcagca gaaggggatc gaccgtctcg ctgcggcgtt cgccgaattg
     3721 cgggcttcgc gcgtgccatt cgatgcccgg gcgatcggcg gggaaatcct cgcggatgcc
     3781 accttgtcgt ggacggatcg cctgaaggac ctcggcgtcg aggtgcgccc gccggtgttc
     3841 gcaagcaagg acctgatcaa ggccctcggt tgggcggatg tccttctgat gccgtcgcgc
     3901 tgggagggcg ctcccttgat gatcgccgag gcgcagcagc tcggctgcgt cccgatcgcg
     3961 acggcagtcg gtgccgtcga cgaactcata accaacggcg aggacggcat cctcatcgag
     4021 gccgccgccg atccccaggt ggtccgggac atggcaaagg ccatcgagga ggtggccaac
     4081 aaccgccaga tgctcgcgcc cctcatggag gggtgcctga gaacggcggc acgccgctca
     4141 tggacgtcct ccttttccga gttcctcggc tggtgcgacc gttccgtgaa caattcatcg
     4201 ctttcgcgcg caacggtcat ccgtgggcgc gaagcatcca atcccggagt tgcggccgtg
     4261 ggctgaggcc ccgccggttt caagaacttc gggatcctct agagtcgacc tgcaggcatg
     4321 caagcttgcg gccgcgtcgt gactgggaaa accctggcga ctagtcttgg actcctgttg
     4381 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     4441 cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt acgtatttaa
     4501 atttgacagc ttatcatcga taaactgtaa tgcggtagtt tatcaaggac gaatggtgca
     4561 aaacctttcg cggtatggca tgatagcgcc cggaacacac agaattcatt aaagaggaga
     4621 aaggtaccat gttggatatg ggacaagatc ggccgatcga tggaagtggg gcacccgggg
     4681 cagacgacac acgcgttgag gtgcaaccgc cggcgcagtg ggtcctcgac ctgatcgagg
     4741 ccagcccgat cgcatcggtc gtgtccgatc cgcgtctcgc cgacaatccg ctgatcgcca
     4801 tcaaccaggc cttcaccgac ctgaccggct attccgaaga agaatgcgtc ggccgcaatt
     4861 gccgattcct ggcaggttcc ggcaccgagc cgtggctgac cgacaagatc cgccaaggcg
     4921 tgcgcgagca caagccggtg ctggtcgaga tcctgaacta caagaaggac ggcacgccgt
     4981 tccgcaatgc cgtgctcgtt gcaccgatct acgatgacga cgacgagctt ctctatttcc
     5041 tcggcagcca ggtcgaagtc gacgacgacc agcccaacat gggcatggcg cgccgcgaac
     5101 gcgccgcgga aatgctcaag acgctgtcgc cgcgccagct cgaggttacg acgctggtgg
     5161 catcgggctt gcgcaacaag gaagtggcgg cccggctcgg cctgtcggag aaaaccgtca
     5221 agatgcaccg cgggctggtg atggaaaagc tcaacctgaa gaccagcgcc gatctggtgc
     5281 gcattgccgt cgaagccgga atctgacttc tatgaaatct aacaatgcgc tcatcgtcat
     5341 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
     5401 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     5461 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     5521 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     5581 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
     5641 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     5701 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     5761 cgggggactg ttgggcgcca tctccctgca cgcaccattc cttgcggcgg cggtgctcaa
     5821 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
     5881 tccgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcaggac
     5941 cattgtggcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     6001 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     6061 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     6121 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     6181 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     6241 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     6301 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     6361 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     6421 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     6481 gagccgggcc acctcgacct gagacaattg tccttttccg ctgcataacc ctgcttcggg
     6541 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     6601 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     6661 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     6721 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccctac cggcgcggca
     6781 gcgttacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg
     6841 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca
     6901 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg
     6961 gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca
     7021 gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca
     7081 actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc
     7141 cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt
     7201 attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca
     7261 cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt
     7321 tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac
     7381 cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca
     7441 tgcggtaggg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag
     7501 cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta
     7561 cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt
     7621 tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag
     7681 ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc
     7741 ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc
     7801 ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgccc
     7861 ttcgactgcc cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct
     7921 gcgcggtcgc tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag
     7981 cgcgcaggcc gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa
     8041 ggccgcaggc cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg
     8101 gcaatccctg tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg
     8161 gttgtccacg ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat
     8221 atccacgggc tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc
     8281 gtaggggggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc
     8341 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     8401 tgatgccttt
//
