LOCUS       sequence-369323-       15389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15389)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 15389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDONR221-HUWE1_UBA/UIM/UBM-mut
FEATURES             Location/Qualifiers
     source          1..15389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(13618..13717)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(13829..13848)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13840..14649
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13907..13926)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      14739..15327
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15228..15247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atgaaagtag acaggactaa actgaagaag acacctactg aggctcctgc agactgcaga
      541 gccttaatag acaaactcaa agtttgtaat gatgagcaac ttctcttgga actgcagcag
      601 atcaaaacat ggaacattgg aaagtgcgag ttatatcact gggtggacct gttggaccgc
      661 ttcgatggaa tactggcaga tgctggacag acagtggaga atatgtcatg gatgctcgta
      721 tgtgataggc cagaaagaga gcaactgaaa atgcttctct tggctgtgtt gaacttcaca
      781 gccttgctca ttgagtacag cttttcccgg catctgtaca gttccataga gcatttgaca
      841 actttattgg cttcctctga tatgcaagtg gtgctggcag tcctcaatct cctatatgta
      901 tttagcaaaa gatcaaacta catcactcgt ctgggatctg acaagaggac cccgctgcta
      961 actcggctac aacatttggc agagagctgg ggtggaaagg agaatggctt tggacttgca
     1021 gaatgttgca gagacttgca tatgatgaaa tatccaccca gtgcaactac actacacttt
     1081 gaattctatg cagatcctgg ggccgaggtc aaaattgaga aaaggacaac tagtaacaca
     1141 ctacattata ttcacataga gcaacttgac aagatttcag aaagcccttc tgaaatcatg
     1201 gaatctctta ccaaaatgta cagcattcct aaggataagc agatgctgtt atttacacac
     1261 atacgactgg cccatggctt ttctaatcac aggaagcgat tgcaggcagt tcaggccaga
     1321 ctgcatgcaa tatctatatt agtgtattcc aatgccttgc aggaatcagc aaacagtatc
     1381 ttgtataatg gcttgataga ggagttagtg gacgtgctgc aaataacgga taagcagctt
     1441 atggagatta aagcagcttc tttacgaaca ttaacatcaa ttgtccactt ggagagaact
     1501 cccaaactca gcagtattat tgactgtact ggaactgcct cctaccatgg atttttgcca
     1561 gtgcttgtaa ggaactgtat ccaggccatg attgatcctt ccatggatcc ataccctcac
     1621 cagtttgcca ctgctctctt ctctttttta taccatctgg ccagctacga tgctggtggt
     1681 gaagccttgg tctcctgtgg aatgatggaa gccttattga aggtcataaa gtttcttggc
     1741 gatgaacagg accagataac atttgtcacc agagccgtca gagtggttga ccttatcacc
     1801 aacctggata tggcagcttt tcaatcccat agtggacttt ctatcttcat ttatagactt
     1861 gagcatgaag tagatctgtg ccgaaaagaa tgtccgtttg tgatcaagcc aaagatccag
     1921 agacccaata ctacacaaga aggagaggaa atggaaactg atatggatgg agtccagtgt
     1981 attccacaac gagcagcact tctgaaatcc atgttgaatt tcctcaagaa ggccatccaa
     2041 gaccctgctt tctcagatgg catacgacat gtgatggatg gttctctgcc tacctccctg
     2101 aaacacatca tcagcaatgc agaatactat ggcccatcac tcttcctcct agctactgaa
     2161 gtggtgactg tgtttgtatt tcaagaacca tcactgctct cctcactcca ggacaatgga
     2221 ttgacagatg tcatgctgca tgcactgctt atcaaagatg ttcctgctac ccgtgaagtc
     2281 cttggctccc tcccaaatgt attcagtgca ctctgtttga atgcccgagg tcttcagtct
     2341 tttgttcagt gtcagccttt tgaacgcctc ttcaaagttc ttctgtctcc agattacctc
     2401 ccagccatgc ggaggaggag aagttctgat ccccttgggg atactgcatc caacctgggg
     2461 agtgctgtcg atgagctcat gagacatcag cccaccctta aaacagatgc aacgactgcc
     2521 atcatcaagt tacttgaaga aatctgtaat cttggaaggg accccaaata catctgtcag
     2581 aagccatcga tccagaaggc agatggcact gccactgctc ctcccccaag gtctaatcat
     2641 gccgcagaag aagcctctag tgaggatgag gaggaagagg aagtacaggc catgcagagc
     2701 tttaattcta cccagcaaaa tgaaactgag cctaatcagc aggttgttgg tacagaggaa
     2761 cgtattccta ttcccctcat ggattacatc cttaatgtga tgaaatttgt ggaatctatt
     2821 ctgagcaaca atacaacaga tgaccactgc caggaatttg tgaatcagaa aggactgttg
     2881 cctttggtta ccattttggg tcttcccaat ctgcccattg actttcccac atctgctgcc
     2941 tgtcaggctg ttgcaggtgt ctgcaaatcc atattgacac tgtcacatga acccaaagtc
     3001 cttcaagagg gtctccttca gttggactcc atcctctcct ccctggagcc cttacaccgc
     3061 cccattgaat cccctggggg ctcagtgttg ttgcgagaac tggcttgcgc aggcaatgtt
     3121 gctgatgcta ccctctcagc ccaggccaca cctctgctgc atgcactcac tgctgcccat
     3181 gcctacatca tgatgtttgt tcatacttgc agagttggac agagtgaaat tcgttccatc
     3241 tccgtaaacc agtggggctc tcaattgggt ctgagtgttt tgagcaagct gagccagtta
     3301 tactgttccc tggtgtggga aagcactgtc ctcctctctc tgtgtacccc aaacagccta
     3361 ccatctgggt gtgaatttgg ccaggcagat atgcagaaac tggttccaaa ggatgagaag
     3421 gcaggtacga cccagggcgg aaaaagatca gatggggaac aggatggagc agctggaagt
     3481 atggatgctt ctacccaggg cttattagaa ggcattgggc tagatggtga cacattggct
     3541 cccatggaga cagatgaacc tactgcttca gactctaagg gcaaatctaa aatcacacca
     3601 gcaatggctg ccagaattaa gcaaatcaag cctttgttat cagcttcctc cagattaggc
     3661 cgagcacttg ctgagctatt tggacttctt gttaaacttt gtgtgggatc tcctgtccgc
     3721 cagagaagga gccatcatgc tgccagcacc actacagcac cgacacctgc cgcgcgatca
     3781 acagcctcag ctctcactaa gctcttgact aaggggttat cttggcagcc cccaccatat
     3841 acacctactc cccgattcag gctgacattc ttcatctgtt cagttggttt cacatcccca
     3901 atgctgtttg atgagaggaa gtatccctac cacctcatgc tgcaaaaatt tctctgctcc
     3961 ggaggccaca atgctctttt tgaaactttc aactgggctc tgtccatggg aggtaaagtt
     4021 cctgtttctg agggattgga acactcagac ttgcctgatg gcacaggaga attcctagat
     4081 gcctggctta tgctggtgga gaagatggtg aatcccacca cggtgcttga atctccacat
     4141 tcgctgcctg ccaaattgcc tggaggtgtc cagaactttc cccagttcag tgcactgcgc
     4201 ttccttgtgg taactcagaa agcagccttt acttgcatca aaaacttatg gaaccggaaa
     4261 cccctgaagg tatatggtgg acgaatggct gaatcgatgc tggccattct atgccacatc
     4321 ctccgaggag aacctgtgat tcgagagaga ctaagcaagg agaaggaggg gtctcgagga
     4381 gaagaggata cagggcaaga ggaaggtggc tcccgccggg aacctcaagt caaccagcaa
     4441 caactgcaac agctcatgga cgcgggcgcg acaagggaac atgcaatgga ggcactgttg
     4501 aacaccagca ccatggagca ggccacagag taccttttaa cccaccctcc tccaatcatg
     4561 ggaggagttg ttcgggatct cagcatgtct gaagaggacc agatgatgag aggcattgct
     4621 atggcgctgg gacaggatat tccaatggat caaagggcag agtcacctga ggaagttgct
     4681 tgccggaagg aggaagagga acggaaagct cgggaaaagc aggaggagga agaggctaaa
     4741 tgtctagaga agttccagga tgctgacccg ttggaacaag atgagctcca cactttcaca
     4801 gatactatgt tgccaggctg cttccacctt cttgatgagc tgccagacac agtataccgt
     4861 gtgtgtgacc tgatcatgac agcaatcaaa cgtaatggag cagattatcg tgacatgatt
     4921 ctgaagcaag tagtcaatca ggtgtgggaa gctgctgatg tattgatcaa agctgctctt
     4981 cccctgacaa caagtgacac aaaaaccgtg tcagagtgga taagtcagat ggccacactg
     5041 ccccaggcct ccaatttggc tactagaatc ttgcttttaa cgctactttt tgaggagttg
     5101 aagctacctt gtgcttgggt ggttgaatca agtggcatcc ttaatgtcct aatcaaactc
     5161 ttggaagtgg ttcagccctg cctccaggca gccaaggagc agaaggaagt ccagacccca
     5221 aagtggatca caccagtgtt gctcctgatt gatttctatg aaaagacagc catctcctca
     5281 aaaaggagag cccagatgac taagtacctg caatccaaca gcaacaactg gcgctggttt
     5341 gatgatcgct ctgggcgttg gtgtagttac agtgcaagca acaatagcac tattgattct
     5401 gcctggaaat ctggagagac aagcgtgcga ttcactgcag gccgaagaag atacacggtc
     5461 caattcacta caatggtgca ggttaatgag gaaacaggga accgacgccc tgtgatgctg
     5521 actctcctca gggtacctcg gctgaataaa aattcaaaaa acagcaatgg acaggaacta
     5581 gagaagacgc tggaagaaag caaagaaatg gatatcaaac gtaaagaaaa taaaggcaat
     5641 gatacccctt tggccctaga gagtacaaac actgaaaagg agacaagcct ggaggaaaca
     5701 aaaatcgggg agatcctgat ccagggcttg acagaagata tggtgactgt tttaatccgg
     5761 gcctgcgtga gcatgctggg agtccctgtg gacccagata ctttgcatgc cacccttcgt
     5821 ctctgtctga ggctcacccg ggaccacaaa tatgccatga tgtttgcaga actgaagagt
     5881 acccgcatga tcttgaattt gacccagagc tcaggcttca atgggtttac tcccctggtc
     5941 acccttctct taagacacat cattgaggac ccctgtaccc ttcgtcatac catggaaaag
     6001 gttgttcgct cagcagctac aagtggagct ggtagcacta cctctggtgt tgtgtctggc
     6061 agcctcggct ctcgggagat caactacatc cttcgtgtcc ttgggccagc cgcatgccgc
     6121 aatccagaca tattcacaga agtggccaac tgctgtatcc gcatcgccct tcctgcccct
     6181 cgaggctcag gaactgcttc agatgatgaa tttgagaatc ttagaattaa aggccctaat
     6241 gctgtacagc tggtgaagac cacccctttg aagccctcac ctctgcctgt catccctgat
     6301 actatcaagg aagtgatcta tgatatgctg aatgctctgg ctgcatacca tgctccagag
     6361 gaagccgata aatctgatcc taaacctggg gttatgaccc aagaggttgg ccagctcctg
     6421 caagacatgg gtgatgatgt ataccagcag taccggtcac ttacgcgtca gagcagtgac
     6481 tttgatacgc agtcaggttt ttccattaat agtcaggtct ttgctgcaga tggtgcctcc
     6541 actgagactt ccgcatctgg gacctcccaa ggagaggctt caactccaga ggagtctcga
     6601 gatgggaaga aagataaaga aggggaccgg gcctctgagg aaggcaaaca gaaaggcaag
     6661 ggcagcaaac ctttaatgcc tacctccact atccttcgtc ttctggcaga gttggtgagg
     6721 tcctatgttg gtattgctac cctgattgcc aactacagct acactgtggg ccagtctgaa
     6781 ctgatcaaag aggactgcag tgtgctagct tttgttctgg accacctgct cccacatacc
     6841 cagaatgcag aagacaagga cacccctgcc ttggcccgcc tgttcctcgc aagcctggct
     6901 gctgcaggga gtggcacaga tgcccaggtg gccctagtga atgaagtaaa agcagccctt
     6961 ggacgggcac tggctatggc tgagagtaca gagaaacatg ccaggcttca ggcagtgatg
     7021 tgtatcatca gtactatcat ggagtcctgc ccctccacct ccagcttcta cagcagtgcc
     7081 acagcgaaga cccagcacaa tggcatgaac aacatcattc ggcttttcct gaagaaggga
     7141 ctggttaatg acctggccag agtacctcac agcttagacc tgtccagtcc caacatggcc
     7201 aacacagtca atgctgctct gaagcctttg gaaacacttt cccggattgt gaaccagccc
     7261 agtagccttt ttggcagcaa gagtgcttct agcaagaaca agtctgagca ggatgcccaa
     7321 ggagcctctc aagattccag tagcaaccag caggacccag gcgagcctgg ggaagcagaa
     7381 gtgcaggagg aggatcatga tgtcactcag acagaggtgg cagatgggga tatcatggat
     7441 ggggaggctg aaaccgactc agtggtgatt gctgggcagc ctgaggtgct cagttcacaa
     7501 gagatgcagg ttgagaatga gctggaggac ctgatagatg agttgcttga gagggatggc
     7561 ggatctggga acagtacaat tatagtgagc agaagtggag aggatgaatc acaagaggac
     7621 gtgctgatgg atgaagctcc ttccaacctc agccaagctt ccaccttgca ggccaaccga
     7681 gaagattcca tgaatatcct ggaccctgag gatgaggagg agcacactca ggaagaggac
     7741 agcagtggca gtaacgagga tgaggatgat agtcaggatg aagaggagga ggaggaggaa
     7801 gatgaggaag atgatcagga ggatgatgaa ggtgaagagg gagatgaaga cgatgacgac
     7861 gatggctctg agatggaatt ggatgaggat tatcctgata tgaacgcttc tcccttggtc
     7921 cgatttgagc gctttgaccg ggaggatgat ctcatcattg agtttgacaa catgttctcc
     7981 agtgctacag acatcccccc atccccagga aatatcccta ccacccatcc actgatggtg
     8041 cgccatgcag accacagttc tctgacactg ggcagtggct cttcaacaac tcgtctcacc
     8101 cagggcatcg ggcgcagtca gaggacccta aggcagctga cggccaatac tggccacacc
     8161 attcatgttc actaccctgg gaatcgccag cccaaccctc ctcttatact gcagaggttg
     8221 cttggtccct cagctgctgc tgacatcctt cagctgagca gcagccttcc cctacaaagc
     8281 cggggtcggg cccgcctcct ggtaggcaac gatgacgtcc acatcatcgc ccgttctgat
     8341 gatgagctgc tggatgactt tttccatgat cagagcacag ctaccagcca agcaggaacc
     8401 ctgtccagca tccccacagc cctgacccgc tggacagaag aatgcaaagt tctcgatgct
     8461 gagagcatgc atgactgtgt ttcagtggtt aaagtgtcca ttgtcaatca cctggaattc
     8521 ctgagggatg aggagctgga agaaaggcga gagaagcgca ggaaacaact ggctgaggaa
     8581 gaaacaaaga taactgataa aggcaaagaa gataaggaga acagggatca gagtgcacag
     8641 tgtactgcat ctaagtcaaa tgactccact gaacagaatc tctcagatgg gacgcctatg
     8701 cctgacagct acccaacaac cccatcttca actgatgcag ctacatctga gtccaaggag
     8761 acccttggca ctctgcaatc ctcacaacag caaccaacac tcccaacccc accagctttg
     8821 ggagaggttc ctcaggagct gcagtctcca gctggagaag ggggcagctc tacacagcta
     8881 ttgatgcctg tagagccaga ggaattgggt cccacaaggc caagtgggga agcagaaaca
     8941 actcagatgg agttatcccc agctcccact ataacctcac tttccccaga gagagctgag
     9001 gattctgatg cactgacggc tgtcagcagt cagctagaag gctctcctat ggatacaagc
     9061 agcctggctt cctgtacctt agaggaggct gtgggtgaca cttcagcagc tggcagttct
     9121 gagcagccca gagcaggcag ctccactcct ggggatgccc caccagctgt ggcggaagtg
     9181 caaggcagga gtgatgggtc aggggaatct gcccagccac ctgaggacag ctccccacct
     9241 gcatcctctg agagctcttc caccagagat tctgccgtgg ccatttctgg cgccgattcc
     9301 cgaggaatcc tagaagagcc gttgccttca acaagcagtg aagaagaaga tccccttgcg
     9361 ggtatcagtc tccctgaagg tgtggacccc tcttttctgg ctgccgcggc agatgacatc
     9421 cgtcgggaag ttctacagaa ccagctaggc attcgtccac caacccggac tgccccctcc
     9481 acaaatagct cagcgcctgc agtggtgggg aatcctggtg tgactgaggt gagtcccgag
     9541 tttcttgcgg cactgccacc cgcgatccaa gaggaagtac tggcacagca gagagctgag
     9601 cagcagcgac gagaactagc acagaatgcc agctcagaca cccctatgga ccctgtgacc
     9661 ttcatccaga ctctgccctc agacctgcgc cgtagtgtcc tagaggatat ggaggacagt
     9721 gtgttagctg tgatgccacc tgacattgca gctgaggctc aagccctgag acgagagcaa
     9781 gaagcccggc agcgacagct catgcatgag cgtctgtttg ggcacagtag cacctccgca
     9841 ctctctgcta ttctccgaag cccggctttc accagtcgct taagtggcaa ccgtggggtc
     9901 cagtatactc gccttgctgt gcagagaggt ggcaccttcc agatgggggg tagcagcagc
     9961 cataacaggc cttctggcag taatgtagat actctcctcc gcctccgagg acggctcctt
    10021 ctggaccacg aagccctttc ttgtctcttg gtcctacttt ttgtggatga gccaaagctc
    10081 aatactagcc gtctacaccg agtactgaga aatctctgct accatgccca gacccgccac
    10141 tgggtcatcc gcagtctgct ctccatcttg cagcgcagca gtgagagtga gctatgcatt
    10201 gaaacaccca aactcactac aagtgaggaa aagggcaaaa agtcgagcaa gagctgtggg
    10261 tcaagtagcc atgagaaccg tcccctggac ctgctacaca agatggagtc aaagagctcc
    10321 aaccagcttt cctggctctc agtatccatg gatgcagccc taggctgcag gactaatata
    10381 tttcagatcc agcgttcagg ggggcgtaaa cataccgaga agcatgcaag cggtggctcc
    10441 accgtccaca tccatcccca agctgctcct gttgtctgca gacacgtttt ggatacactc
    10501 attcaattgg ccaaggtatt tcccagccac ttcacacagc agcggaccaa agaaacaaac
    10561 tgtgagagtg atcgggaaag gggcaataag gcctgtagcc catgctcctc acagtcctcc
    10621 agcagtggca tttgcacaga cttctgggac ttattggtaa aactggacaa catgaatgtc
    10681 agccggaaag gcaagaactc cgtgaagtca gtgccagtga gcgctggcgg tgagggggaa
    10741 acctctccat acagcctcga ggcctctcca ctggggcagc tcatgaacat gttgtcacac
    10801 ccagtcatcc gccggagctc tctcttaact gagaaactcc tcagactcct ttctctcatc
    10861 tcaattgctc tcccagaaaa caaggtgtca gaagcacagg ctaattctgg cagcggtgct
    10921 tcctccacca ccactgccac ctcaaccaca tctaccacca ccaccactgc cgcctccacc
    10981 acgcccacac cccctactgc acccacccct gtcacttctg ctccagccct ggttgctgcc
    11041 acggctattt ccaccattgt cgtagctgct tcgaccacag tgactacccc cacgactgct
    11101 accactactg tttcaatttc tcccactact aagggcagca aatctccagc gaaggtgagt
    11161 gatgggggca gcagcagtac agactttaag atggtgtcct ctggcctcac tgaaaaccag
    11221 ctacagctct ctgtagaggt gttgacatcc cactcttgtt ctgaggaagg cttagaggat
    11281 gcagccaacg tactactgca gctctcccgg ggggactctg ggacccggga cactgttctc
    11341 aagctgctac tgaatggagc ccgccatctg ggttataccc tttgtaaaca aataggtacc
    11401 ctgctggccg agctgcggga atacaacctc gagcagcagc ggcgagccca atgtgaaacc
    11461 ctctctcctg atggcctgcc tgaggagcag ccacagacca ccaagctgaa gggcaaaatg
    11521 cagagcaggt ttgacatggc tgagaatgtg gtaattgtgg catctcagaa gcgacctttg
    11581 ggtggccggg agctccagct gccttctatg tccatgttga catccaagac atctacccag
    11641 aagttcttct tgagggtact acaggtcatc atccagctcc gggacgacac gcgccgggct
    11701 aacaagaaag ccaagcagac aggcaggcta ggttcctccg gtttaggctc agctagcagc
    11761 atccaggcag ctgttcggca gctggaggct gaggctgatg ccattataca aatggtacgt
    11821 gagggtcaaa gggcgcggag acagcaacaa gcagcaacgt cggagtctag ccagtcagag
    11881 gcgtctgtcc ggagggagga atcacccatg gatgtggacc agccatctcc cagtgctcaa
    11941 gatactcaat ccattgcctc cgatggaacc ccacaggggg agaaggaaaa ggaagaaaga
    12001 ccacctgagt tacccctgct cagcgagcag ctgagtttgg acgagctgtg ggacatgctt
    12061 ggggagtgtc taaaggaact agaggaatcc catgaccagc atgcggtgct agtgctacag
    12121 cctgctgtcg aggccttctt tctggtccat gccacagagc gggagagcaa gcctcctgtc
    12181 cgagacaccc gtgagagcca gctggcacac atcaaggacg agcctcctcc actctcccct
    12241 gcccccttaa ccccagccac gccttcctcc cttgacccat tcttctcccg ggagccctca
    12301 tctatgcaca tctcctcaag cctgccccct gacacacaga agttccttcg ctttgcagag
    12361 actcaccgca ctgtgttaaa ccagatccta cggcagtcca cgacccacct tgctgatggg
    12421 ccttttgctg tcctggtaga ctacattcgt gtcctcgact ttgatgtcaa gcgcaaatat
    12481 ttccgccaag agctggagcg tttagatgag gggctccgga aagaagacat ggctgtgcat
    12541 gtccgtcgtg accatgtgtt tgaagactcc tatcgtgagc tgcatcgcaa atcccccgaa
    12601 gaaatgaaga atcgattgta tatagtattt gaaggagaag aagggcagga tgctggcggg
    12661 ctcctgcggg agtggtatat gatcatctct cgagagatgt ttaaccctat gtatgccttg
    12721 ttccgtacct cacctggtga tcgagtcacc tacaccatca atccatcttc ccactgcaac
    12781 cccaaccatc tgtcctattt taaatttgtc ggacgcattg tggccaaagc tgtatatgac
    12841 aaccgtcttc tggagtgcta ctttactcga tccttttaca aacacatctt gggcaagtca
    12901 gtcagatata cagatatgga gagtgaagat taccacttct accaaggtct ggtttatctg
    12961 ctggaaaatg atgtctccac actaggctat gacctcacct tcagcactga ggtccaagag
    13021 tttggagttt gtgaagttcg tgacctcaaa cccaatgggg ccaacatctt ggtaacagag
    13081 gagaataaga aggagtatgt acacctggta tgccagatga gaatgacagg agccatccgc
    13141 aagcagttgg cggctttctt agaaggcttc tatgagatca ttccaaagcg cctcatttcc
    13201 atcttcactg agcaggagtt agagctgctt atatcaggac tgcccaccat tgacatcgat
    13261 gatctgaaat ccaacactga ataccacaag taccagtcca actctattca gatccagtgg
    13321 ttctggagag cattgcgttc tttcgatcaa gctgaccgtg ccaagttcct ccagtttgtc
    13381 acgggtactt ccaaggtacc cctgcaaggc tttgctgccc tcgaaggcat gaatggcatt
    13441 cagaagtttc agatccatcg agatgatagg tccacagatc gcctgccttc agctcacaca
    13501 tgttttaatc agctggatct gcctgcctat gagagctttg agaagctccg ccacatgcta
    13561 ctgttggcta tccaggagtg ctctgaaggc tttgggctgg cctaataagc ggccgcgacc
    13621 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
    13681 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc
    13741 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
    13801 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
    13861 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
    13921 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
    13981 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
    14041 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
    14101 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
    14161 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gtccctgcgc
    14221 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
    14281 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
    14341 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
    14401 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
    14461 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
    14521 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
    14581 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
    14641 tttttctaat cagaattggt taattggttg taacattatt cagattgggc cccgttccac
    14701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    14761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    14821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    14881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    14941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    15001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    15061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    15121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    15181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    15241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    15301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    15361 gccttttgct ggccttttgc tcacatgtt
//
