LOCUS       sequence-369324-        2034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT015
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    60..78
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    123..141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(401..435)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(506..1165)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1080..1099
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1347..1935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1836..1855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacggttgt aggatactcc ctatcagtga tagagaaatc tatgcggcat
       61 ccctatcagt gatagagatc ggtaacggag tccctatcag tgatagagaa gtggtgttca
      121 gttccctatc agtgatagag aagtttgaca ctatccctat cagtgataga gagaacagca
      181 atgactccct atcagtgata gagatcaaat ggcgtatccc tatcagtgat agagataatt
      241 aacttgtaat attctaatca attgatataa atagaggcct ggtatcggct gtcatgatct
      301 catctcttcc ctatttacaa aaaaactgca agtatagaca ataaaacaac agcacaaata
      361 tcaaaaaagg aattaaaaag atctatgtga gaccagacca ataaaaaacg cccggcggca
      421 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      481 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      541 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      601 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      661 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      721 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      781 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      841 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      901 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      961 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1021 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1081 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1141 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1201 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1261 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1321 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1381 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1441 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1501 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1561 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1621 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1681 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1741 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1801 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1861 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1921 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1981 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgt
//
