LOCUS       sequence-369328-        9684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs1 from Erwinia piriflorinigrans CFBP5888.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: EpAvs1
FEATURES             Location/Qualifiers
     source          1..9684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(7..24)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(141..686)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(221..240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        911..1015
                     /gene="bla"
                     /label=AmpR promoter
     terminator      8270..8317
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8537..9196)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9111..9130
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(9197..9299)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
       61 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
      121 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
      181 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      241 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
      301 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
      361 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
      421 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
      481 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
      541 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
      601 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
      661 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
      721 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
      781 agttgtaatt ctcatgttag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
      841 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
      901 tgcgttgcgc cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      961 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatggt
     1021 agcgataaaa atgtatccgg caaaggatgg ggatgctttt cttattattt gcgatgagga
     1081 aaaaagtgca tttctgattg acggaggcta cgcggaaacg ttcaggcaac atattttgcc
     1141 tgacttacgt gagctgagtt ttaacggtta ccggttacgt ctggtcatgg caacacatat
     1201 tgattcagat cacattggtg gtctcgtgga cttctttctt gtaaatggac acgcagcaga
     1261 gcctgcagtg attactgttg accgcgtatg gcacaacagc ctcagggcga tgacgagacc
     1321 cgaaaataat gcacaaaaag tggattcccg agaaatcact gactttttga gacggagata
     1381 tcatgtcgaa gccgataaag ccaaaccgca tgaaatcagc gcgcgtcagg ggagttcact
     1441 ggctgccagc cttctggctg gcgattatca ttggaatgag ggaaaagggt atcagtgtat
     1501 ctgcaccggt acctccattc ccaacttgat gtgcgataac agtctaacaa ttctgagccc
     1561 ctctaaggag agaatttcag cgctctgcct gtggtggcgc agacaacttg catcgctggg
     1621 cttttcggga cggtcctcct cgagtgaggc atttgatgat gctttcgaat ttttttgtaa
     1681 aagggaagca tctcaggttc ctcttccgca tgtcatcaat gcaagaacac cgttgcttga
     1741 gagggattat gcacgggata cctcgccaac aaatggcagt tcgatagcgt tcagtctggt
     1801 gctcaataag aagagaatat tgatgctagg agatgcctgg gcggaagaag ttgtgacatc
     1861 tctgggtgcc agtggggcgt cccatcattt tgatatcatt aaaatctcac atcacggtag
     1921 tattagaaac acaagcccga atcttttaaa gatcatagat gctcctgtgt acctgatctc
     1981 aaccgacgga aaaaagcatg ccagacaccc taacctggcg gttctgaaag cgattgtgga
     2041 cagacctgcg gcgtttacgc gaacgctcta ttttaactat gccaacagcg catctgcttt
     2101 tatgaaaaat tacctttctg caagtggtgc acaattcaga atcattgaag gatcaacgga
     2161 ttggataaca ctgtgagata tgctgctact gaaactgaaa taaggaacgc aactgtactc
     2221 attgaatgcg cgggttacac tggttccgga accctgatcg cagcagacaa ggtccttacg
     2281 gctgcacatt gtgtagtatc ggatgatcct gagacaccaa ttacagtgac attttttggt
     2341 gcggatgaag acgtctgtgt caatgcgaca atttcagaaa tagatacatc gtgcgatgcc
     2401 tgtctgctaa cactttctga ctctgtcgac attccgccta ttacacttat gacacagccg
     2461 gagcgagagg gaagccaatg gaaagccttt ggctatccgg catcacgcaa tgggccatca
     2521 cattatcttc atggcactat aagtcagatt ttaccaaggc ttttccatgg cgttgatatg
     2581 gatttgtcgg tcagtgccga ttgtgttctg gaagagtaca gtggagtttc tggtgccgcc
     2641 attctatcag aaaataaatg cattgcgatg gtgcgcatca ggatggatgg tggactaggt
     2701 gcagtaagtc ttgataagtt aagcggtttg ctgattcgaa acggcctcat cccagatgac
     2761 attgcatccc tgccagattc atcactgtcg ggtgaagttg tcctgaaccg cacagaattt
     2821 cgcgacaact ttgaatcgtt cgtcctggag cacaagggac gtgcagtgct tttggaaggt
     2881 agtcccggct ctggtaagac taccttctgc cgccattatc agccccgtag tgagcaactc
     2941 gcagtggcgg gtgtctatga atttacaccg gaagacggtg ctggtacgac attcaaaatt
     3001 cttcctgagg tatttgccga ttggctgcat aaccaggttt ctatactgct ttcaggtagg
     3061 cctgctcgca gggaggaaac agaaaagatc aatctgaccc aaaaggtgtc tgaccttcta
     3121 catactttct cagattactg gaagcacaaa ggaaaatatg gcgtcatttt cattgatgct
     3181 gtgaatgagg caagcgagtg cggggatgag gcagtatcgc gctttacagc attactgccg
     3241 gtgacacttc cggagaacgt caaacttgtt ttcaccgcac catcattatc atcagctggt
     3301 aaggctttcc ggcactggct cacacctcag gattgtatca gcctaacgct tttaagccat
     3361 agggaggtgt tacagctaac agctcgagag cttaaaactt ccgccccttc tttgtcacta
     3421 ctcacacgag ttagtgatat agctcagggc catccacttt atctccgata cattcttggg
     3481 tatctgaaag cgaatccgga tcaggttaat ctggagatat tcccggtttt cagtggcagc
     3541 attgaaacct actacgaaag gctctggcag gggctggtta aggatgagag cgctgtaaat
     3601 ctgctcggta ttctctcgcg gatgcgctgg ggcattgata tttcatcact gatccctgtt
     3661 ctaacaccgc aggaacagac ggtgtttgtt ccaacccttg accgtattca gcatctgctt
     3721 cttaatgata aatcatcagc attgtgccac caatcatttg cggcgtttat caacagtaaa
     3781 acggcggtaa ttaactcgct gctgcacgga cgccttgccg acttctgcct taccagtgga
     3841 gagagttatg gcctgattaa tcgcgcttat cacctgctcc tagcctctca cgacagacat
     3901 cctgaagccg cattggtgtg cacgcaggaa tgggctgacg cctgtatcgt caagggggct
     3961 cagccggata ttctaattca cgatatccgt cagaccctga agaacacgct tattcgtgcc
     4021 gatgcagtgg catcgattcg tctgttgctg cttttccaac gcatgacctt cagacaccat
     4081 tttttgtttc tgcagtcagc ttatcactca ggccttgccc tggctgcact tggcagaccg
     4141 gatgaggccc ttgagcagct cataccatct ggaagcctcg ttgttgatgc agttgatgca
     4201 attgtcagcg cacagactct cgcgcgtatg ggaaacagtg aacacgcgct gaagctattg
     4261 gaaaaggtga agtcagctgt cgaccaagaa tttgaacgca atcccgtcaa tctatctgat
     4321 tttatcggcc tttccctggc ttgggtgaga gctgagctga tggctggggt ggttgatggc
     4381 cacggacgca cacgcgaggt tgttgagtat ttgtacggtt gtgggcaagt cgttcgcgat
     4441 aattttgaac aatcagcgca tagtaaatca gcatatacac gcgcttttta tcctcttcag
     4501 gcagaaatgg aagccgtgaa catagccttt aatgaccgct ccgtatcttt acggacggtt
     4561 aaagaaaagt ttggtagctt accggaaaat attcttgatc tgatgctcag ttcagttatg
     4621 cgggcacatg acatcattct gcaacatcag ttgccgatgc cccagcatgc tttgcaaccc
     4681 gtttggtaca atctggacag attacttcat actgatattc cgtattcgaa cgaaattcgt
     4741 tttaattcat taagtagcct tatttttttc aatgcgcctt ctgctcttat tatcaggatg
     4801 gcgggggtat tttctttcga agtagtaccc gaaataacgt tgctcaatga agaaaatgag
     4861 atagcagcag acagcattga cgttagtgaa cagggacaac tctggctggt gagcgcctac
     4921 cttaatgaaa cgcaaccctg tcccgatatt aaacatccga gtcagggatg ttctgaatgg
     4981 ctcaagacat tgactgaggc tattttttgg tacagcgggc aggcgcgccg ggcagttatt
     5041 gacggcaacg atgagaaaaa agaactgctt ttagtcaagg tgcagaatga tattctccct
     5101 gctctttcgt actcgctgga agagcgcatg gcatggccga attcatgggc aatgcctgaa
     5161 cagattatcc ccatgattta cgaagagtta gtaaacatgt tcggcgcatg ctggcccgat
     5221 aagatatcag tgatcactga tttcattctg gctcatacgc ctcagcaatg tggactttat
     5281 tccgaggggt acaggcgttt actgaacaga gttattcaga ctcttctaaa tgagcatcgg
     5341 tttttggggc aatctgatac gacatttcaa ctacttgaga cgttgcatgc gtttgtttct
     5401 gcttttactg agaatcggca ggagctggtt cctgaattac tgaatattat tccagcttat
     5461 attagccttg atgctcctca gctggcacag gacacttaca ctgagctttt aggtgtgtcg
     5521 atgggccctg actggtacaa agaagaccaa tttgccctca tgacaactat gctgcgcgtg
     5581 ataccacagc atacagacac aaatactaca ctttcacaag ttgcaggatt ccttgaacat
     5641 gcttcgggtg aaatgacatt taggcgttat gttaggcagg aaaaatcaca gtttattggc
     5701 gaacttattc gtcgtgggaa ttatgcacac gggtttaact attatcgtca gcagtcctgc
     5761 ggatcccatg aggaaatgct cacccaactt agccacccag ctgcagatag ccctcatcca
     5821 ttgaaaggca tgcggttccc ggggggagcg ctggatgagg aacatgctgt agaatgcatt
     5881 gtcagtgaac tgcgaaacag agtcgactgg cggcttcgct ggggacttct tgaaatattc
     5941 agctttggca gtattggtaa tcttgcagtg ccctttgctg aacttatcaa tgaattttct
     6001 gcagacactg aagaccttaa tgaaataccc aaaaggttgc acaacatttt acatggtgat
     6061 gtgcctttct cagaacacag aaattttatc aaaaatttca cagagcacct tgcagacaac
     6121 cataagccac tctttgctga atttatcagt ttgctatccg aagacactag cgataacgac
     6181 gttaagcctc ccccctctgg tgatgctaac cagaagggta ctgatacctc agatgatgtg
     6241 gcaatgcagc caggactttt tgggaagcgt tctgcgatca atagggctga agcctgcatg
     6301 gaaaatgccc gaaaagccgc agcacgcaga aacacagttc gtgcaagtga gttagccgtt
     6361 gaaagcctgc atataattca ggatggtgac tggtcagtct ggagaaagaa caaccatctg
     6421 gcggaactta cacggacgta catattggac aactctgcgg atgcaggttc ggtcattcgt
     6481 gcttatgctt cgcttgtaga aaaagaacgt tatgccccgg catgggtaat tgctagtcat
     6541 ctcatcgaaa tagcagccag taaattctct gatcaagaag cccaagctat taaccagatc
     6601 gtacttgaac acaaccgcca catgcttggg aataccgaag cggatgctgc gcatttttct
     6661 tttcttaatg aacctgatac ctcagatgca ggtgaagaaa cactctattt tctgttttgg
     6721 ctgctggaac acccactgaa attcagacgc gaacgggctc tggaagtact gaagtggctt
     6781 gcatcagacg atgataagat tctgggccaa tgcgtgacgg aggcactcgt ttcagacatt
     6841 gcctcacgag ctgaagcact aatggcattg acagactggg tgtcagctag atctcctcag
     6901 cgaatatggg actttatagt taaagagcgc agcctttttg aatggcttga aggcactact
     6961 gcactaagcc aagtccatct cctggagcga gtaaccagca gagcgggatt tgttttaaga
     7021 aatgagattg ccgcatttga gcgaccccga aagcttttac tgacatcaga agcctctgga
     7081 caacggaata ttccagaaaa tttaccaaca tgggtgcaat ccttgtcgca gacccttgcc
     7141 gtgatggaaa agcagggaat agatatccca gctttgctta ccttactcga aaaacgggtt
     7201 ttacagcaga gtggattggc tgatatcacg gtggcttttg agctggaaaa gttacttgcg
     7261 cgtggtttta ctgtgaatag aacaccaagt caccatcgct gggagacgat ggtgcgattt
     7321 gcattaaacc agatcataca tgaggcggcc gcacaggatg aactgcaaaa cattgaaccc
     7381 ttgctacgtg cctggaaccc cgcgtcagag gagtgtgttg agccgtggga ggtttgtaac
     7441 cgggcaaaac agattatctg cgctgttatg gaaggtagac atcagcaagc ttcgggcata
     7501 gaggatggct ttttcttgca ttatcttgat gaagtggagg tttcccgaga aggtcaaacg
     7561 catctggtgg aaatctcagc ggtgttaacg acagctcata atggtcatga gagccttaga
     7621 ccaggtgcag aaagcgaatt taatgcaaca cagacacctg atatagagcg gacgcttagt
     7681 gtgcacctta catgccagcg agtcaaaatg cagcctttgc tttttggggg agctacgcct
     7741 gccgcagtgt cgaaaaagtt tatgcagatg actggaacgt tgccttcaga ctttattcgc
     7801 aggcaatggc gaagcgggcg ttctcttagt aaaaacagat ggggggaacc aataagcaga
     7861 ggaagtctgt tactcatgaa aagaacaact accctccctc caggactggg cttagcgtgg
     7921 tatgtcactg tcgatgggaa gttgatgaat atattttcat atgccccgag gaggagataa
     7981 tgaaatacag ttcaatggaa acgccaaaaa cgcgagagga atttgaggct cgctgttttc
     8041 acctgctcaa tgcgatcaag ttaggacggt atcatggcat tccgggtgaa ggtaacaaag
     8101 agcaggttcc ttttctccct aacggacgag ttgatctggc aaacattgat accatgactc
     8161 gcctctcgat gaactcgtta tatgatttcc actataacag ggataattat ccgcagtttg
     8221 atctctctga aaatgacgag aatgaagagg ctacggattg acgcaataac tagcataacc
     8281 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
     8341 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     8401 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     8461 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat
     8521 aactgcctta aaaaaattac gccccgccct gccactcatc gcaatactgt tgtaattcat
     8581 ttaacattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
     8641 gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg ggggcgaaga
     8701 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     8761 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     8821 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     8881 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     8941 cccatatcac cagctcaccg tctttcatcg ccatacggaa ctctggatga gcattcatca
     9001 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     9061 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     9121 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     9181 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     9241 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     9301 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     9361 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     9421 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     9481 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     9541 agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
     9601 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
     9661 gaatatgtga tacaggatat attc
//
