LOCUS       sequence-369329-        7443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs2 from Escherichia coli NCTC9087.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: EcAvs2
FEATURES             Location/Qualifiers
     source          1..7443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5275..5322
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5542..6201)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6116..6135
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6202..6304)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6696..6713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6830..7375)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6910..6929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc
       61 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
      121 taatgagtga gctaacttac attaattgcg ttgcgcaggg atttccacca cctcccaccg
      181 accatctaag actttatgcc actgtcccta ggactgctat gtactaggag cggatgttaa
      241 actcagactc gtttcagcta cattgcgttt tgaataatat tccatcataa taactctttg
      301 aaaaatgtga tcttttcatt tataacactg atgacttgct tatctcattg ggatatcgga
      361 ggagaatact taactatgac aagcccgatt attatgacac tggctatatt atatagattg
      421 atattaaaat gtaggattag gttcttgcca aggtgtcaag atttacagat aggtttaaaa
      481 ccatataaat atgttttacg gtgagataca atacatattg taaggcataa acgcttggta
      541 aaattttaat tattggaaga agctaatcat ggaacccata tcaattacag tggcaactta
      601 tgtagcaact aaacttattg atcaattcat ctctcaagaa ggatatggtt gtattaagaa
      661 agcattattc ccccaaaaaa gatatgtgga tagattatat caactaattg aagagacggc
      721 aattgagttt gaagaaacat atccagtaga aagtggagca ataccatttt atcattccga
      781 accattgttt gagatgttga atgagcacat cttttttaaa gagttccctg acaaagagat
      841 attattagac aagttcaaag aatatccaag tatcactccc ccaactcaac aacaactcag
      901 ccttttttat gagatgttat cattaaaaat caataattgt tcgaagttaa aaaagctaca
      961 tatcgaagaa acgtataaag aaaaaatatt cgatattaat gaagagctca ttcaagtcaa
     1021 acttatttta cggtctatag atgagaaact aacttttcac ttaagtgatg attggttaaa
     1081 tgaaaaaaat agtcaagcaa tagctgactt gggaggtcga tacacacccg aactcaacgt
     1141 aaagctagaa atagcagaga tatttgatgg cctcggtaga actaatgatt tttctaaaat
     1201 attttattcg catatagata gctttctggt cgctggaaag aaattacata gttgcgatgt
     1261 aatttcctca gaattatttg aaataaacca gtccttaaaa gaaatttctg atatatatca
     1321 ggagattaat ttttctaaat tagatgaaat ccctataaat aaatttaata actatgtttc
     1381 tagctgccag acagctattg gcggagcggt atcaatattg tgggaactcc gagaaaagtc
     1441 agagcaagta ggtgaaacca agcattacag tgataagtat tcatctactc tgcgaatgct
     1501 tcgggaattt gactatgcgt gcaatgaatt acgtatattc attaattcaa caacagtgaa
     1561 gttggctaac aacccattct tacttctcga aggaaaagca ggaattggta agtctcattt
     1621 actggctgat gtgattaaaa atcgaattgc ttctgggtat ccttcactac tcatactagg
     1681 gcaacaactt acttcagatg aatctccatg gtcacaaatc ttcaagagat tacagcttaa
     1741 aatcacttct cgtgaattcc tagaaaaact gaatttatat ggcaaaaaaa caggaaaaag
     1801 agtcttagtt tttattgatg ctattaatga aggtaatgga aataaattct ggaatgacaa
     1861 tattaacagt tttgtcgatg aaatcagatg ctttgaatgg cttggtctga taatgtcagt
     1921 cagaacaaca tatagaaatg taacaatttc acatgagaat gttgtgcgaa ataattttga
     1981 aattcatgaa catattggat tccagaacgt tgagttggaa gcggttagtc tattttatga
     2041 ttattacaat attgagaggc cttcatctcc taaccttaat ccagagttta aaaatcctct
     2101 atttcttaag ttattgtgtg aaggcattaa gaaaaatggt ttaaccaaag tgcctgttgg
     2161 atttaatggg atttcaaata tttttaactt tttagttgaa ggggtaaata aatcattagc
     2221 atcgccaaaa aaatatgcat tcgatcccag ttttcctctt gttaaagatg ctctcaatga
     2281 aatcataaaa ttcaaattag agattggtcg taatagtatt tcacttaaag atgctcactc
     2341 agtggttcaa tctgtagtta atgattatgt tgctgataaa accttcctca gcgccttgat
     2401 tgacgaagga ttattgacta aaggcatagt gagaaatgat gataattcta ctgaggaagt
     2461 agtttatgtg gcttttgaaa ggtttgatga tcatttaact gttaattttt tattaaatga
     2521 tgttgaaaat atcgaaagtg aatttaagcc tgatggtcgt ctgaaaaaat attttcatga
     2581 tgaatgtgat ttttatataa aatcgggaat agtagaggcg ttgtctattc aattgccaga
     2641 aaggtatgaa aaagagcttt atgaatttct gccggagttc agcaataatc ttaaattact
     2701 agaagccttt attgatagct tgatatggcg cgatattaag gctattgatt tcgaaaaaat
     2761 tagacctttc atcaatgaac atgtttttaa atttaaagat agttttgatc atttcctcga
     2821 ggcagtgatc tctatttcag gtttagttgg ccatcccttt aatgctaatt tcttgcatga
     2881 ttggctaaaa gattattctt tggcaaatcg agattcgttt tggactacag aacttaaata
     2941 taaatatagt gaagactcag catttaggca tctaatcgat tgggcatggg ccagaacaga
     3001 taaaagcttt gtttcggatg agtcaatcga gctagttgca actagtttat gctggttttt
     3061 aacttctagt aaccgagaac ttcgagattg ctcaactaag gctttagtga gtttactcga
     3121 gccaagaatt cctgtattga gaaaaataat tgataagttt tatggtgtaa atgatcctta
     3181 cgtttgggaa agaatatttg cagttgcatt aggctgtaca ttgcgaactg ataatattaa
     3241 agaactaaaa tatttagccg aaactgttta ccaaaaggta ttttgttcta agtatgtgta
     3301 tccaaatata ttacttagag attatgctag agagattatt gaatttgcta atcatcttgg
     3361 attggaactt gaaagcattg aattatccaa gactagacca ccctacaaca gcatttggcc
     3421 tgacaagatt ccttcaaaag aggaactaga gtccctttat gataaagaac cttatcggga
     3481 actctggagc tctattatgg aagatggtga cttttcacga tatactattg gaacaaatta
     3541 taatcattct gattggtctg gttgcaagtt taatgaaacc cctgttgacc gtaagcaagt
     3601 ttttaaaact ttcaaatgta aactaactga tcaacaaaaa gacttgtatg atgccacaga
     3661 tcctttcatt tatgatgata aatgcgaagg aattaaattt ggtcgtgtgg tcggtagaaa
     3721 agcacaggaa gaaataaagg cgagcaagaa attatttaag aattcattgt catacgatct
     3781 gttaagtgag tttgaaaatg aaatagagcc atacctggat cataataata atctgctgga
     3841 aactgataaa cactttgatc ttcgactagc tcaacaattt atattcaatc gtgttataga
     3901 gcttggttgg gatccggaga agcatggtaa ttttgaccaa caaataggaa ctggacgtgg
     3961 acgtagagag gcattccaag aacggattgg taaaaaatac caatggattg cttattatga
     4021 atacatggca aggctagccg ataattttac tcgttttgaa ggttatggtg acgaacgaaa
     4081 ggaaaatcca taccaagggc catgggagcc ttacgtaaga gatatagatc ccactatctt
     4141 acttaaagaa actggaacga aaaaaataag caataaagaa atgtggtggc ttaatgatga
     4201 agtgtttgat tggacttgct ctaatgaaga ctgggttaaa agttctacta ctataactaa
     4261 ttcatatgct tttattgaag ttaaagatga taatggtgat gaatggatag tattagaaag
     4321 tcatccatca tggaaagaac caaaaattat tggaaacgat gattgggggc acccacgaaa
     4381 agaggtttgg tatcagatca gaagttatat cgttaaagtt gaagaatttg aaaattttag
     4441 atgttgggca atagctcaag actttatggg caggtggatg ccggaatgta ctgatagata
     4501 ccaattattt aatagggagt actattggtc cgaagcattt aagtctttta aatcagatta
     4561 ttatggtgga tctgactgga cttcggtaac agaccgggag tctggagcta agatagctga
     4621 tgttagtgtc acttcgatta attatttgtg ggaagaggag ttcgacaaat caaaaataga
     4681 aactttgaat tttttgaagc ctagtaactt aatctttgaa aagatgggat taaaaagtgg
     4741 ggaagtagag ggtagcttca atgatgaaaa tggaactatg gtttgctttg cagctgaagc
     4801 tgtatatgct tcaaagccgc atctacttgt taaaaaagaa ccatttttaa caatgttaag
     4861 ggacaatggt tttgaaatcg tttggacatt attaggtgaa aagggcgtta tagggggctc
     4921 actcatatca agtcatcatt atggtcgaca ggagtttagt ggagcatttt attatgaaga
     4981 cagtcagcta acaggaagtc ataaaactag ctttacgaga taaaaatgaa tctcagagct
     5041 gaatatataa gtagtattag aaaccgggtt atacttaaga aatcaatctt aagtgtggca
     5101 gtcgaatggt agctaatatg ctagcggcgc taatgcctgt ttgttgctca taacaggcat
     5161 tcactttagt tatggcagaa aagtatacat gctgggttgg gaaagtgtga aagaaaggaa
     5221 gattgctgcg ccgtttgtcg tcacgtttat cttcattggc tatgcacgca ataactagca
     5281 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt
     5341 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     5401 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     5461 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg tttaagggca
     5521 ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcaat actgttgtaa
     5581 ttcatttaac attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     5641 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatagtga aaacgggggc
     5701 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     5761 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     5821 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     5881 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     5941 actatcccat atcaccagct caccgtcttt catcgccata cggaactctg gatgagcatt
     6001 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     6061 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     6121 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     6181 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     6241 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     6301 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     6361 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
     6421 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     6481 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
     6541 gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac
     6601 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt
     6661 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg
     6721 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat
     6781 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc
     6841 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     6901 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     6961 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     7021 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     7081 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     7141 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     7201 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     7261 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     7321 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     7381 tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt
     7441 agc
//
