LOCUS       sequence-369330-        2781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis
            MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT017
FEATURES             Location/Qualifiers
     source          1..2781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1108..1128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(1148..1182)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1253..1912)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1827..1846
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2094..2682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2583..2602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgaagcc agttacatta tacgacgttg ctgaatacgc tggtgtttct
       61 taccaaactg tttccagagt tgttaatcaa gcctctcacg tttctgctaa gaccagagaa
      121 aaagttgaag ctgctatggc tgaattgaac tacattccaa atagagtcgc tcaacaattg
      181 gctggtaaac agtctttgtt gattggtgtt gctacttctt ctttggcttt acatgctcca
      241 tctcaaatag ttgctgccat taagtcaaga gctgatcaat tgggtgcttc cgttgttgtt
      301 tctatggttg aaagatcagg tgttgaagct tgtaaagctg ctgttcataa tttgttggcc
      361 caaagagttt ccggtttgat tattaactac ccattggatg atcaagatgc cattgctgtt
      421 gaagcagctt gtactaatgt tccagctttg ttcttggatg tctctgatca aactccaatc
      481 aactccatca tcttctcaca tgaagatggt actagattgg gtgttgaaca tttggttgct
      541 ttgggtcatc aacaaattgc tttgttggct ggtccattgt catctgtttc agctagattg
      601 agattagctg gttggcataa gtacttgacc agaaatcaaa ttcaacctat cgctgaaaga
      661 gaaggcgatt ggtctgctat gtctggtttt caacaaacca tgcagatgtt gaacgaaggt
      721 atagttccaa cagctatgtt ggttgctaat gatcaaatgg ctttgggtgc tatgagagct
      781 attactgaat ctggtttgag agttggtgcc gatatttctg ttgttggtta cgatgatacc
      841 gaggattctt cttgttatat tccaccattg accaccatca agcaagattt cagattattg
      901 ggtcaaacct ccgttgatag gttgttgcaa ttgtctcaag gtcaagctgt taagggtaat
      961 caattattgc cagtctcctt ggttaagaga aagactactt tggctccaaa cactcaaact
     1021 gcttctccaa gagctttggc tgattctttg atgcaattgg ctagacaagt ttccagattg
     1081 gaatcaggtc aaggttctgg ttctccacca aaaaagaaaa gaaaggttgg atcctgagac
     1141 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1201 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1261 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1321 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1381 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1441 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1501 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1561 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1621 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1681 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1741 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1801 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1861 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1921 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1981 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     2041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2761 cctgattctg tggataaccg t
//
