LOCUS       sequence-369331-        6569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Avs2 from Escherichia coli NCTC10650.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Ec2Avs2
FEATURES             Location/Qualifiers
     source          1..6569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3522..3569
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3789..4448)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4363..4382
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4449..4551)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4943..4960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5077..5622)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5157..5176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgggactggt gacttttgcg agtggtacga aacatcatta acaccaatcg ttcgagagtt
       61 ccattcactg aatggatacg gacgcaaatc gctggatata ctcggcgaaa cccgtgctac
      121 atctgccacg gcattgattg aagaaataat tgcctactgt gagagcatga gagataacaa
      181 tgtcacggcc acatcggtta cagatctttc cgtcgctctg tcatccctat tgacactttt
      241 cgctgatgcc cgccatgctc aagaagataa attttatgaa aagcatggca agcatagtga
      301 tacagaatcg ttccgacagt tccacgcaga gtatatgtgt gcatttcctg ccggagatat
      361 ggatgcggcg agaaaatggg aagagcaggc gcagcaactg caaaatttgc tgacttctca
      421 ggtcattggt gccgcaacag cacattcctt actgctggtt gggccagcgg gtatcggcaa
      481 aacccacgcg attgtcagcg cagcattgcg tcgactggaa catggtggtt tttcactggt
      541 cgtctttgga gacgactttg gcaaagcaga gccttgggaa gtgctacgca gtaaaatagg
      601 gctgggtgcc gccatcgatc gttcgacatt atttgaatgc atacaggcct gcgccgaaca
      661 tactggctta ccttttgtca tttatatcga tgcattgaac gaaagcccgc gagaagtgcg
      721 ctggaaggac aagcttcccg aattgctcgc tcaatgcaag tcttatccag acatcaaaat
      781 ctgcgtttca acccgagata cctatcgcaa tcttgtggtc gattcacgct ttccagggtt
      841 tgctttcgaa cacatcggtt tttcaggaca tcaattcgaa gcggtacaag ctttcgcagc
      901 ctactatgag ctggatgcag agattacacc acttttttca cccgaactcg gtaatccttt
      961 atttttacac ttggcctgta aaacgctaaa gggcgaaggc cgtgacagtc tggatatttc
     1021 tttgccgggt tttacctctc tgtttcaagg acatctcaaa cattgcgatg ttttaattcg
     1081 agaacgcctc cactacgcaa accctcgtaa tctggtaagg gctgcaatga tggcactcgc
     1141 gaaaaccctg acacatgagt tgccgcagaa ccgaacgtgg gaaacctgtt gcgaagcact
     1201 gagcaaaata gtgggaactg agaccacacc tgaatccttt ttaaatgcat tggcacatga
     1261 aggcctcatt atcctttctg ttgtagatga ggataccttc ctgatccgtc tgggttatca
     1321 acgctacggt gacatactcc gtgctatcag ccttgtggaa actcttgatt cggatacagt
     1381 aaaactagcg gagaaaattg cagcgttaac agaagaagat gctggattgc tggaagctct
     1441 tgccgccgtg ctgccagaga aaactgctct tgaaattact gctgaagaag taggattacc
     1501 atccgaacaa gcccataagc tgttcatcca gtcattggtt tggcgctccc gacaaagtgt
     1561 agtggaagaa attgatgaac acatccatgc agcactgcat acacctggat tatgggagtc
     1621 ggtttatgaa gcgctgtttt cacttagtct ggttcctgac catcgtctaa acgcaactaa
     1681 ctggctgggg ccatttttac ggcagtcatc cttagctgaa cgtgacacct acttgtcatt
     1741 agctgcgctg ggatcatttg ataataagac tgctgtctat tcactcatcc atgcagcact
     1801 atttgctgac ataacccatt ggcctgctga aagccggagg ctggccagtc taacacttgc
     1861 ctggctcact tcgtgtgctg accgccgaat cagggattta tcctcaaaag ggctaagcag
     1921 aatcctggca aactacccgg agaactgcca aacagtaatc agtgaatttg catattgtga
     1981 tgatgattac gtattagagc gtattagcct tgctatctac agtgcatgct tattgtcata
     2041 ccaacgcaga aatgcgttta tgccagcgct ccctggtcta ttaagcattg cgtcagatag
     2101 caagaatatt ctgctccggg atacggttca gctattagta aacttgttga aaacaggaga
     2161 atttcccaca gccgtaacaa gccaattaca gcattaccag acaaacgtat cattaccatc
     2221 acgatggcct gtactggcgg atgtcaaacc cctcctagat ctggaacatt taccatcaaa
     2281 catggtgctc tggggagaat ccatggcccc ggatttctgg cgttatcagg tggaatcgaa
     2341 gatttccggc tttgacttgg agagcgccaa tatcagccat gaaaacattg cctgttggtt
     2401 aatgcgagaa gcacttaatt taggatatcc cggttataac cactgcgcgc tcaattatga
     2461 tcgccatatc gggagtcagt atggctcggg acggggtaga aaagggtatg ctgaccgact
     2521 cggtaaaaaa tattactgga tcgccttaca tcgactactg ggcattctgg ccagtaatgt
     2581 tcccgcactg gaagacccat attccgacta cgaacctaca agtgatcttc tatggtcagt
     2641 cgacgtccgt aaagttgacc tgaccgatgt acgcgatatc accgcagaag gtgtctatcc
     2701 agtactgatg gaggaaacaa attatgcatt ccctgaccac aattcagata tcaaaggttg
     2761 ggttaggacc gatgattttc caccttatga agcttgtctt attcgaactg acgaggaagg
     2821 agagcagtgg gtagcgcttt cacatagcta ttgggatgac gataaagcgc cgaatgaaaa
     2881 tagctgggat tccccgtact tgggagtgcg tgcttcctac tcaagcgcac tcataaatga
     2941 aagcatccag aactttaaac agaaaagatc acgcgatatt ttccaatata atcagggaag
     3001 tagttgttat cgcggttatc ttgctgaata tcctgacagc ccggtataca aacaacttct
     3061 taatagtgat gaagatagtg aagcgtttaa ttttacagaa gtcagtttac tgcgcggaaa
     3121 cgaatgggaa tacgactact catataccat gcccgagcgc caggataacc tcattgcgcc
     3181 atgcctggga attattcaaa aactcgaact tttatgggat tgtcaaagcg gttgggttga
     3241 tcattctggc aaacttatcg ccttccatca aaaaggtgta aaacaacgcg gacttttcat
     3301 ccatcgttcg gcattgaacg cctatctgtc cataacaggt gaagagctta tacatcgccg
     3361 ttttgctaac agaggatatt ttgatttagc tggtcgtaat agcacgcaaa tagacctgaa
     3421 aacttggatc cagtaccggg cagacaaggc accggtagtt ttacgagaag aggaactgcc
     3481 gtttaactgc tgacaacgat acttattaag taacgcaata actagcataa ccccttgggg
     3541 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
     3601 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     3661 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     3721 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
     3781 taaaaaaatt acgccccgcc ctgccactca tcgcaatact gttgtaattc atttaacatt
     3841 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
     3901 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
     3961 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     4021 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     4081 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     4141 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     4201 accagctcac cgtctttcat cgccatacgg aactctggat gagcattcat caggcgggca
     4261 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     4321 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     4381 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     4441 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     4501 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     4561 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     4621 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
     4681 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
     4741 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
     4801 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
     4861 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
     4921 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     4981 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
     5041 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
     5101 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     5161 ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     5221 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     5281 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     5341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     5401 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     5461 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     5521 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     5581 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
     5641 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
     5701 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
     5761 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
     5821 gctaacttac attaattgcg ttgcgcactt acctcgcttc aataccaata catttttaat
     5881 gggtataata tgtgactgct tttgccgcat tattgacagg aacaaggact ggtgatgaat
     5941 attgatttca gtttaattcg tagcgccccc aaaagccgta acgatagctt tgaagcactc
     6001 gccgtacagt tatttaggaa aacctgtcga gtaccgacaa attcaacatt tattagtctg
     6061 cgtggagatg gtggagacgg tggcgttgag gcatatttcc gctcaccgga cggtgccgta
     6121 ttcggtgttc aggcaaaata ctttttccag cttgcttccg cagagcttac acagattgat
     6181 agttccctta aagctgcgct aagcaaccat cccacactaa ccgaatactg gatttatata
     6241 ccgtttgacc tgaccgggcg tgttgctgcg ggaaagcgag gaaaaagcca ggcggaacgc
     6301 tttgaagaat ggaaaagtaa agtcgaatcg gaagcgtcag cgaaagggaa gtcactttct
     6361 attgtccttt gtaccgctgc tgttatctgc aatcaattac ttgagataga cccttacgga
     6421 gggatgcgca ggtattggtt tgatgacacg ttgctgacaa cagctcaaat tcaacaatgt
     6481 ctggaggacg ccattgcttt tgccgggcca agatatactt caatgctgga tgtggtgacg
     6541 aatgctcatg tcggcctgga tttctttgg
//
