LOCUS       sequence-369334-       18080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of FLAG-HUWE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_CMVT_FLAG-HUWE1
FEATURES             Location/Qualifiers
     source          1..18080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             147..170
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(13407..13427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13825..13836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13903..13920)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13909..14116
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(14164..14169,14170..14186,14187..14192)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             14362..14895
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  14962..15185
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(15249..15837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15329..15348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15876..16667,16668..16736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16499..16518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16737..16808)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  16833..16998
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        17092..17395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        17396..17599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     17550..17570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaaag
       61 ttggccaggc ctagttggcc aggcctagtg ccgccaccat gggtgattac aaggatgacg
      121 atgacaaggg cggtggtagc ggtgggctgg aagttctgtt ccaggggccc gtcgacatga
      181 aagtagacag gactaaactg aagaagacac ctactgaggc tcctgcagac tgcagagcct
      241 taatagacaa actcaaagtt tgtaatgatg agcaacttct cttggaactg cagcagatca
      301 aaacatggaa cattggaaag tgcgagttat atcactgggt ggacctgttg gaccgcttcg
      361 atggaatact ggcagatgct ggacagacag tggagaatat gtcatggatg ctcgtatgtg
      421 ataggccaga aagagagcaa ctgaaaatgc ttctcttggc tgtgttgaac ttcacagcct
      481 tgctcattga gtacagcttt tcccggcatc tgtacagttc catagagcat ttgacaactt
      541 tattggcttc ctctgatatg caagtggtgc tggcagtcct caatctccta tatgtattta
      601 gcaaaagatc aaactacatc actcgtctgg gatctgacaa gaggaccccg ctgctaactc
      661 ggctacaaca tttggcagag agctggggtg gaaaggagaa tggctttgga cttgcagaat
      721 gttgcagaga cttgcatatg atgaaatatc cacccagtgc aactacacta cactttgaat
      781 tctatgcaga tcctggggcc gaggtcaaaa ttgagaaaag gacaactagt aacacactac
      841 attatattca catagagcaa cttgacaaga tttcagaaag cccttctgaa atcatggaat
      901 ctcttaccaa aatgtacagc attcctaagg ataagcagat gctgttattt acacacatac
      961 gactggccca tggcttttct aatcacagga agcgattgca ggcagttcag gccagactgc
     1021 atgcaatatc tatattagtg tattccaatg ccttgcagga atcagcaaac agtatcttgt
     1081 ataatggctt gatagaggag ttagtggacg tgctgcaaat aacggataag cagcttatgg
     1141 agattaaagc agcttcttta cgaacattaa catcaattgt ccacttggag agaactccca
     1201 aactcagcag tattattgac tgtactggaa ctgcctccta ccatggattt ttgccagtgc
     1261 ttgtaaggaa ctgtatccag gccatgattg atccttccat ggatccatac cctcaccagt
     1321 ttgccactgc tctcttctct tttttatacc atctggccag ctacgatgct ggtggtgaag
     1381 ccttggtctc ctgtggaatg atggaagcct tattgaaggt cataaagttt cttggcgatg
     1441 aacaggacca gataacattt gtcaccagag ccgtcagagt ggttgacctt atcaccaacc
     1501 tggatatggc agcttttcaa tcccatagtg gactttctat cttcatttat agacttgagc
     1561 atgaagtaga tctgtgccga aaagaatgtc cgtttgtgat caagccaaag atccagagac
     1621 ccaatactac acaagaagga gaggaaatgg aaactgatat ggatggagtc cagtgtattc
     1681 cacaacgagc agcacttctg aaatccatgt tgaatttcct caagaaggcc atccaagacc
     1741 ctgctttctc agatggcata cgacatgtga tggatggttc tctgcctacc tccctgaaac
     1801 acatcatcag caatgcagaa tactatggcc catcactctt cctcctagct actgaagtgg
     1861 tgactgtgtt tgtatttcaa gaaccatcac tgctctcctc actccaggac aatggattga
     1921 cagatgtcat gctgcatgca ctgcttatca aagatgttcc tgctacccgt gaagtccttg
     1981 gctccctccc aaatgtattc agtgcactct gtttgaatgc ccgaggtctt cagtcttttg
     2041 ttcagtgtca gccttttgaa cgcctcttca aagttcttct gtctccagat tacctcccag
     2101 ccatgcggag gaggagaagt tctgatcccc ttggggatac tgcatccaac ctggggagtg
     2161 ctgtcgatga gctcatgaga catcagccca cccttaaaac agatgcaacg actgccatca
     2221 tcaagttact tgaagaaatc tgtaatcttg gaagggaccc caaatacatc tgtcagaagc
     2281 catcgatcca gaaggcagat ggcactgcca ctgctcctcc cccaaggtct aatcatgccg
     2341 cagaagaagc ctctagtgag gatgaggagg aagaggaagt acaggccatg cagagcttta
     2401 attctaccca gcaaaatgaa actgagccta atcagcaggt tgttggtaca gaggaacgta
     2461 ttcctattcc cctcatggat tacatcctta atgtgatgaa atttgtggaa tctattctga
     2521 gcaacaatac aacagatgac cactgccagg aatttgtgaa tcagaaagga ctgttgcctt
     2581 tggttaccat tttgggtctt cccaatctgc ccattgactt tcccacatct gctgcctgtc
     2641 aggctgttgc aggtgtctgc aaatccatat tgacactgtc acatgaaccc aaagtccttc
     2701 aagagggtct ccttcagttg gactccatcc tctcctccct ggagccctta caccgcccca
     2761 ttgaatcccc tgggggctca gtgttgttgc gagaactggc ttgcgcaggc aatgttgctg
     2821 atgctaccct ctcagcccag gccacacctc tgctgcatgc actcactgct gcccatgcct
     2881 acatcatgat gtttgttcat acttgcagag ttggacagag tgaaattcgt tccatctccg
     2941 taaaccagtg gggctctcaa ttgggtctga gtgttttgag caagctgagc cagttatact
     3001 gttccctggt gtgggaaagc actgtcctcc tctctctgtg taccccaaac agcctaccat
     3061 ctgggtgtga atttggccag gcagatatgc agaaactggt tccaaaggat gagaaggcag
     3121 gtacgaccca gggcggaaaa agatcagatg gggaacagga tggagcagct ggaagtatgg
     3181 atgcttctac ccagggctta ttagaaggca ttgggctaga tggtgacaca ttggctccca
     3241 tggagacaga tgaacctact gcttcagact ctaagggcaa atctaaaatc acaccagcaa
     3301 tggctgccag aattaagcaa atcaagcctt tgttatcagc ttcctccaga ttaggccgag
     3361 cacttgctga gctatttgga cttcttgtta aactttgtgt gggatctcct gtccgccaga
     3421 gaaggagcca tcatgctgcc agcaccacta cagcaccgac acctgccgcg cgatcaacag
     3481 cctcagctct cactaagctc ttgactaagg ggttatcttg gcagccccca ccatatacac
     3541 ctactccccg attcaggctg acattcttca tctgttcagt tggtttcaca tccccaatgc
     3601 tgtttgatga gaggaagtat ccctaccacc tcatgctgca aaaatttctc tgctccggag
     3661 gccacaatgc tctttttgaa actttcaact gggctctgtc catgggaggt aaagttcctg
     3721 tttctgaggg attggaacac tcagacttgc ctgatggcac aggagaattc ctagatgcct
     3781 ggcttatgct ggtggagaag atggtgaatc ccaccacggt gcttgaatct ccacattcgc
     3841 tgcctgccaa attgcctgga ggtgtccaga actttcccca gttcagtgca ctgcgcttcc
     3901 ttgtggtaac tcagaaagca gcctttactt gcatcaaaaa cttatggaac cggaaacccc
     3961 tgaaggtata tggtggacga atggctgaat cgatgctggc cattctatgc cacatcctcc
     4021 gaggagaacc tgtgattcga gagagactaa gcaaggagaa ggaggggtct cgaggagaag
     4081 aggatacagg gcaagaggaa ggtggctccc gccgggaacc tcaagtcaac cagcaacaac
     4141 tgcaacagct catggacatg ggcttcacaa gggaacatgc aatggaggca ctgttgaaca
     4201 ccagcaccat ggagcaggcc acagagtacc ttttaaccca ccctcctcca atcatgggag
     4261 gagttgttcg ggatctcagc atgtctgaag aggaccagat gatgagagca attgctatgt
     4321 ctctgggaca ggatattcca atggatcaaa gggcagagtc acctgaggaa gttgcttgcc
     4381 ggaaggagga agaggaacgg aaagctcggg aaaagcagga ggaggaagag gctaaatgtc
     4441 tagagaagtt ccaggatgct gacccgttgg aacaagatga gctccacact ttcacagata
     4501 ctatgttgcc aggctgcttc caccttcttg atgagctgcc agacacagta taccgtgtgt
     4561 gtgacctgat catgacagca atcaaacgta atggagcaga ttatcgtgac atgattctga
     4621 agcaagtagt caatcaggtg tgggaagctg ctgatgtatt gatcaaagct gctcttcccc
     4681 tgacaacaag tgacacaaaa accgtgtcag agtggataag tcagatggcc acactgcccc
     4741 aggcctccaa tttggctact agaatcttgc ttttaacgct actttttgag gagttgaagc
     4801 taccttgtgc ttgggtggtt gaatcaagtg gcatccttaa tgtcctaatc aaactcttgg
     4861 aagtggttca gccctgcctc caggcagcca aggagcagaa ggaagtccag accccaaagt
     4921 ggatcacacc agtgttgctc ctgattgatt tctatgaaaa gacagccatc tcctcaaaaa
     4981 ggagagccca gatgactaag tacctgcaat ccaacagcaa caactggcgc tggtttgatg
     5041 atcgctctgg gcgttggtgt agttacagtg caagcaacaa tagcactatt gattctgcct
     5101 ggaaatctgg agagacaagc gtgcgattca ctgcaggccg aagaagatac acggtccaat
     5161 tcactacaat ggtgcaggtt aatgaggaaa cagggaaccg acgccctgtg atgctgactc
     5221 tcctcagggt acctcggctg aataaaaatt caaaaaacag caatggacag gaactagaga
     5281 agacgctgga agaaagcaaa gaaatggata tcaaacgtaa agaaaataaa ggcaatgata
     5341 cccctttggc cctagagagt acaaacactg aaaaggagac aagcctggag gaaacaaaaa
     5401 tcggggagat cctgatccag ggcttgacag aagatatggt gactgtttta atccgggcct
     5461 gcgtgagcat gctgggagtc cctgtggacc cagatacttt gcatgccacc cttcgtctct
     5521 gtctgaggct cacccgggac cacaaatatg ccatgatgtt tgcagaactg aagagtaccc
     5581 gcatgatctt gaatttgacc cagagctcag gcttcaatgg gtttactccc ctggtcaccc
     5641 ttctcttaag acacatcatt gaggacccct gtacccttcg tcataccatg gaaaaggttg
     5701 ttcgctcagc agctacaagt ggagctggta gcactacctc tggtgttgtg tctggcagcc
     5761 tcggctctcg ggagatcaac tacatccttc gtgtccttgg gccagccgca tgccgcaatc
     5821 cagacatatt cacagaagtg gccaactgct gtatccgcat cgcccttcct gcccctcgag
     5881 gctcaggaac tgcttcagat gatgaatttg agaatcttag aattaaaggc cctaatgctg
     5941 tacagctggt gaagaccacc cctttgaagc cctcacctct gcctgtcatc cctgatacta
     6001 tcaaggaagt gatctatgat atgctgaatg ctctggctgc ataccatgct ccagaggaag
     6061 ccgataaatc tgatcctaaa cctggggtta tgacccaaga ggttggccag ctcctgcaag
     6121 acatgggtga tgatgtatac cagcagtacc ggtcacttac gcgtcagagc agtgactttg
     6181 atacgcagtc aggtttttcc attaatagtc aggtctttgc tgcagatggt gcctccactg
     6241 agacttccgc atctgggacc tcccaaggag aggcttcaac tccagaggag tctcgagatg
     6301 ggaagaaaga taaagaaggg gaccgggcct ctgaggaagg caaacagaaa ggcaagggca
     6361 gcaaaccttt aatgcctacc tccactatcc ttcgtcttct ggcagagttg gtgaggtcct
     6421 atgttggtat tgctaccctg attgccaact acagctacac tgtgggccag tctgaactga
     6481 tcaaagagga ctgcagtgtg ctagcttttg ttctggacca cctgctccca catacccaga
     6541 atgcagaaga caaggacacc cctgccttgg cccgcctgtt cctcgcaagc ctggctgctg
     6601 cagggagtgg cacagatgcc caggtggccc tagtgaatga agtaaaagca gcccttggac
     6661 gggcactggc tatggctgag agtacagaga aacatgccag gcttcaggca gtgatgtgta
     6721 tcatcagtac tatcatggag tcctgcccct ccacctccag cttctacagc agtgccacag
     6781 cgaagaccca gcacaatggc atgaacaaca tcattcggct tttcctgaag aagggactgg
     6841 ttaatgacct ggccagagta cctcacagct tagacctgtc cagtcccaac atggccaaca
     6901 cagtcaatgc tgctctgaag cctttggaaa cactttcccg gattgtgaac cagcccagta
     6961 gcctttttgg cagcaagagt gcttctagca agaacaagtc tgagcaggat gcccaaggag
     7021 cctctcaaga ttccagtagc aaccagcagg acccaggcga gcctggggaa gcagaagtgc
     7081 aggaggagga tcatgatgtc actcagacag aggtggcaga tggggatatc atggatgggg
     7141 aggctgaaac cgactcagtg gtgattgctg ggcagcctga ggtgctcagt tcacaagaga
     7201 tgcaggttga gaatgagctg gaggacctga tagatgagtt gcttgagagg gatggcggat
     7261 ctgggaacag tacaattata gtgagcagaa gtggagagga tgaatcacaa gaggacgtgc
     7321 tgatggatga agctccttcc aacctcagcc aagcttccac cttgcaggcc aaccgagaag
     7381 attccatgaa tatcctggac cctgaggatg aggaggagca cactcaggaa gaggacagca
     7441 gtggcagtaa cgaggatgag gatgatagtc aggatgaaga ggaggaggag gaggaagatg
     7501 aggaagatga tcaggaggat gatgaaggtg aagagggaga tgaagacgat gacgacgatg
     7561 gctctgagat ggaattggat gaggattatc ctgatatgaa cgcttctccc ttggtccgat
     7621 ttgagcgctt tgaccgggag gatgatctca tcattgagtt tgacaacatg ttctccagtg
     7681 ctacagacat ccccccatcc ccaggaaata tccctaccac ccatccactg atggtgcgcc
     7741 atgcagacca cagttctctg acactgggca gtggctcttc aacaactcgt ctcacccagg
     7801 gcatcgggcg cagtcagagg accctaaggc agctgacggc caatactggc cacaccattc
     7861 atgttcacta ccctgggaat cgccagccca accctcctct tatactgcag aggttgcttg
     7921 gtccctcagc tgctgctgac atccttcagc tgagcagcag ccttccccta caaagccggg
     7981 gtcgggcccg cctcctggta ggcaacgatg acgtccacat catcgcccgt tctgatgatg
     8041 agctgctgga tgactttttc catgatcaga gcacagctac cagccaagca ggaaccctgt
     8101 ccagcatccc cacagccctg acccgctgga cagaagaatg caaagttctc gatgctgaga
     8161 gcatgcatga ctgtgtttca gtggttaaag tgtccattgt caatcacctg gaattcctga
     8221 gggatgagga gctggaagaa aggcgagaga agcgcaggaa acaactggct gaggaagaaa
     8281 caaagataac tgataaaggc aaagaagata aggagaacag ggatcagagt gcacagtgta
     8341 ctgcatctaa gtcaaatgac tccactgaac agaatctctc agatgggacg cctatgcctg
     8401 acagctaccc aacaacccca tcttcaactg atgcagctac atctgagtcc aaggagaccc
     8461 ttggcactct gcaatcctca caacagcaac caacactccc aaccccacca gctttgggag
     8521 aggttcctca ggagctgcag tctccagctg gagaaggggg cagctctaca cagctattga
     8581 tgcctgtaga gccagaggaa ttgggtccca caaggccaag tggggaagca gaaacaactc
     8641 agatggagtt atccccagct cccactataa cctcactttc cccagagaga gctgaggatt
     8701 ctgatgcact gacggctgtc agcagtcagc tagaaggctc tcctatggat acaagcagcc
     8761 tggcttcctg taccttagag gaggctgtgg gtgacacttc agcagctggc agttctgagc
     8821 agcccagagc aggcagctcc actcctgggg atgccccacc agctgtggcg gaagtgcaag
     8881 gcaggagtga tgggtcaggg gaatctgccc agccacctga ggacagctcc ccacctgcat
     8941 cctctgagag ctcttccacc agagattctg ccgtggccat ttctggcgcc gattcccgag
     9001 gaatcctaga agagccgttg ccttcaacaa gcagtgaaga agaagatccc cttgcgggta
     9061 tcagtctccc tgaaggtgtg gacccctctt ttctggctgc cctgcctgat gacatccgtc
     9121 gggaagttct acagaaccag ctaggcattc gtccaccaac ccggactgcc ccctccacaa
     9181 atagctcagc gcctgcagtg gtggggaatc ctggtgtgac tgaggtgagt cccgagtttc
     9241 ttgcggcact gccacccgcg atccaagagg aagtactggc acagcagaga gctgagcagc
     9301 agcgacgaga actagcacag aatgccagct cagacacccc tatggaccct gtgaccttca
     9361 tccagactct gccctcagac ctgcgccgta gtgtcctaga ggatatggag gacagtgtgt
     9421 tagctgtgat gccacctgac attgcagctg aggctcaagc cctgagacga gagcaagaag
     9481 cccggcagcg acagctcatg catgagcgtc tgtttgggca cagtagcacc tccgcactct
     9541 ctgctattct ccgaagcccg gctttcacca gtcgcttaag tggcaaccgt ggggtccagt
     9601 atactcgcct tgctgtgcag agaggtggca ccttccagat ggggggtagc agcagccata
     9661 acaggccttc tggcagtaat gtagatactc tcctccgcct ccgaggacgg ctccttctgg
     9721 accacgaagc cctttcttgt ctcttggtcc tactttttgt ggatgagcca aagctcaata
     9781 ctagccgtct acaccgagta ctgagaaatc tctgctacca tgcccagacc cgccactggg
     9841 tcatccgcag tctgctctcc atcttgcagc gcagcagtga gagtgagcta tgcattgaaa
     9901 cacccaaact cactacaagt gaggaaaagg gcaaaaagtc gagcaagagc tgtgggtcaa
     9961 gtagccatga gaaccgtccc ctggacctgc tacacaagat ggagtcaaag agctccaacc
    10021 agctttcctg gctctcagta tccatggatg cagccctagg ctgcaggact aatatatttc
    10081 agatccagcg ttcagggggg cgtaaacata ccgagaagca tgcaagcggt ggctccaccg
    10141 tccacatcca tccccaagct gctcctgttg tctgcagaca cgttttggat acactcattc
    10201 aattggccaa ggtatttccc agccacttca cacagcagcg gaccaaagaa acaaactgtg
    10261 agagtgatcg ggaaaggggc aataaggcct gtagcccatg ctcctcacag tcctccagca
    10321 gtggcatttg cacagacttc tgggacttat tggtaaaact ggacaacatg aatgtcagcc
    10381 ggaaaggcaa gaactccgtg aagtcagtgc cagtgagcgc tggcggtgag ggggaaacct
    10441 ctccatacag cctcgaggcc tctccactgg ggcagctcat gaacatgttg tcacacccag
    10501 tcatccgccg gagctctctc ttaactgaga aactcctcag actcctttct ctcatctcaa
    10561 ttgctctccc agaaaacaag gtgtcagaag cacaggctaa ttctggcagc ggtgcttcct
    10621 ccaccaccac tgccacctca accacatcta ccaccaccac cactgccgcc tccaccacgc
    10681 ccacaccccc tactgcaccc acccctgtca cttctgctcc agccctggtt gctgccacgg
    10741 ctatttccac cattgtcgta gctgcttcga ccacagtgac tacccccacg actgctacca
    10801 ctactgtttc aatttctccc actactaagg gcagcaaatc tccagcgaag gtgagtgatg
    10861 ggggcagcag cagtacagac tttaagatgg tgtcctctgg cctcactgaa aaccagctac
    10921 agctctctgt agaggtgttg acatcccact cttgttctga ggaaggctta gaggatgcag
    10981 ccaacgtact actgcagctc tcccgggggg actctgggac ccgggacact gttctcaagc
    11041 tgctactgaa tggagcccgc catctgggtt ataccctttg taaacaaata ggtaccctgc
    11101 tggccgagct gcgggaatac aacctcgagc agcagcggcg agcccaatgt gaaaccctct
    11161 ctcctgatgg cctgcctgag gagcagccac agaccaccaa gctgaagggc aaaatgcaga
    11221 gcaggtttga catggctgag aatgtggtaa ttgtggcatc tcagaagcga cctttgggtg
    11281 gccgggagct ccagctgcct tctatgtcca tgttgacatc caagacatct acccagaagt
    11341 tcttcttgag ggtactacag gtcatcatcc agctccggga cgacacgcgc cgggctaaca
    11401 agaaagccaa gcagacaggc aggctaggtt cctccggttt aggctcagct agcagcatcc
    11461 aggcagctgt tcggcagctg gaggctgagg ctgatgccat tatacaaatg gtacgtgagg
    11521 gtcaaagggc gcggagacag caacaagcag caacgtcgga gtctagccag tcagaggcgt
    11581 ctgtccggag ggaggaatca cccatggatg tggaccagcc atctcccagt gctcaagata
    11641 ctcaatccat tgcctccgat ggaaccccac agggggagaa ggaaaaggaa gaaagaccac
    11701 ctgagttacc cctgctcagc gagcagctga gtttggacga gctgtgggac atgcttgggg
    11761 agtgtctaaa ggaactagag gaatcccatg accagcatgc ggtgctagtg ctacagcctg
    11821 ctgtcgaggc cttctttctg gtccatgcca cagagcggga gagcaagcct cctgtccgag
    11881 acacccgtga gagccagctg gcacacatca aggacgagcc tcctccactc tcccctgccc
    11941 ccttaacccc agccacgcct tcctcccttg acccattctt ctcccgggag ccctcatcta
    12001 tgcacatctc ctcaagcctg ccccctgaca cacagaagtt ccttcgcttt gcagagactc
    12061 accgcactgt gttaaaccag atcctacggc agtccacgac ccaccttgct gatgggcctt
    12121 ttgctgtcct ggtagactac attcgtgtcc tcgactttga tgtcaagcgc aaatatttcc
    12181 gccaagagct ggagcgttta gatgaggggc tccggaaaga agacatggct gtgcatgtcc
    12241 gtcgtgacca tgtgtttgaa gactcctatc gtgagctgca tcgcaaatcc cccgaagaaa
    12301 tgaagaatcg attgtatata gtatttgaag gagaagaagg gcaggatgct ggcgggctcc
    12361 tgcgggagtg gtatatgatc atctctcgag agatgtttaa ccctatgtat gccttgttcc
    12421 gtacctcacc tggtgatcga gtcacctaca ccatcaatcc atcttcccac tgcaacccca
    12481 accatctgtc ctattttaaa tttgtcggac gcattgtggc caaagctgta tatgacaacc
    12541 gtcttctgga gtgctacttt actcgatcct tttacaaaca catcttgggc aagtcagtca
    12601 gatatacaga tatggagagt gaagattacc acttctacca aggtctggtt tatctgctgg
    12661 aaaatgatgt ctccacacta ggctatgacc tcaccttcag cactgaggtc caagagtttg
    12721 gagtttgtga agttcgtgac ctcaaaccca atggggccaa catcttggta acagaggaga
    12781 ataagaagga gtatgtacac ctggtatgcc agatgagaat gacaggagcc atccgcaagc
    12841 agttggcggc tttcttagaa ggcttctatg agatcattcc aaagcgcctc atttccatct
    12901 tcactgagca ggagttagag ctgcttatat caggactgcc caccattgac atcgatgatc
    12961 tgaaatccaa cactgaatac cacaagtacc agtccaactc tattcagatc cagtggttct
    13021 ggagagcatt gcgttctttc gatcaagctg accgtgccaa gttcctccag tttgtcacgg
    13081 gtacttccaa ggtacccctg caaggctttg ctgccctcga aggcatgaat ggcattcaga
    13141 agtttcagat ccatcgagat gataggtcca cagatcgcct gccttcagct cacacatgtt
    13201 ttaatcagct ggatctgcct gcctatgaga gctttgagaa gctccgccac atgctactgt
    13261 tggctatcca ggagtgctct gaaggctttg ggctggccta ataagcggcc gctgatgata
    13321 atggcctgtg gccgtaatgg cctgtggccg taaaatcaac ctctggatta caaaatttgt
    13381 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    13441 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    13501 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    13561 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    13621 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact cagtgccgcc
    13681 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    13741 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    13801 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    13861 ctgctgccgg ctctgcggcc tcttccgcgg cttcgagatc tgcctcgact gtgccttcta
    13921 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
    13981 ctcccactgt cctttcctaa taaaatgagg aaattgcatc aaattgtctg agtaggtgtc
    14041 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gcagacaata
    14101 gcaggcatgc tggggactcg agttaagggc gaatcgctgg gacgcccgaa cggtggctac
    14161 tcgttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc
    14221 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
    14281 gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca gcaagcgcgt
    14341 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
    14401 cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgtacatg taggctcggc
    14461 cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggtgat
    14521 gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt
    14581 aagacattta ttgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct
    14641 tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc
    14701 tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag
    14761 gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca
    14821 gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca
    14881 agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccgc ggagttgttc
    14941 ggtaaattgt cacaacgccg cgaatatagt ctttacatgc ccttggccac gcccctcttt
    15001 aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca taacaaaagt
    15061 ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat tctgtctagt
    15121 ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt tattgtccgc
    15181 ccacacccgc ttacgcaggg catctgcgca tccacaggaa gccgtaaaaa ggccgcgttg
    15241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    15301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    15361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    15421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    15481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    15541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    15601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    15661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    15721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    15781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    15841 gatcctttga tcttttctac ggggtctgac gcttattacc aatgcttaat cagtgaggca
    15901 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    15961 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgtgac
    16021 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    16081 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    16141 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    16201 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    16261 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    16321 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    16381 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    16441 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    16501 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    16561 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    16621 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    16681 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    16741 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    16801 tttgaatgta tttaggaccc aagtcaaccg gttgtgggcg gacaaaatag ttgggaactg
    16861 ggaggggtgg aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga
    16921 actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta
    16981 gatttcactt atctggttca gcctgcggtc cggattcgcc cttaagctag ctagttatta
    17041 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    17101 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    17161 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    17221 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
    17281 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    17341 atgggacttt cctacttggc agtacatcta cgtattagtc attgctatta ccatggtgat
    17401 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
    17461 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    17521 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
    17581 ggtctatata agcagagctg gtttagtgaa ccgtcagatc ctcactctct tccgcctcgc
    17641 tgtctgcgag ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca
    17701 gtactcttgg atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga
    17761 gcgagtccgc atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt
    17821 cgcaaggtag gctgagcacc gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg
    17881 cggaggtgct gctgatgatg taattaaagt aggcggtgtt gacaccgcgg atggtcgagg
    17941 tgaggtgtgg caggcttgag atccagctgt tggggtgagt actccctctc aaaagcgggc
    18001 attacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
    18061 cccgatctgg ccatacactt
//
