LOCUS       sequence-369336-        9959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector control for pLenti_EF1a constructs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Stransky LA, Vigeant SM, Huang B, West D, Denize T, Walton E, 
            Signoretti S, Kaelin WG Jr
  TITLE     Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not
            require an intact p53 pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120403119. doi: 
            10.1073/pnas.2120403119. Epub 2022 Mar 31.
  PUBMED    35357972
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-04-5"; volume: "119(14):e2120403119. 
            doi"; pages: " 10.1073/pnas.2120403119. Epub 2022 Mar 31"
COMMENT     Sequence Label: pLenti_EF1a_EV
FEATURES             Location/Qualifiers
     source          1..9959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..38
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(104..128)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        197..696
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(232..255)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     629..648
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             714..1739
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             714..1739
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(1770..1803)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1859..2447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1912..1932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2330..2341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2450..2466)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2451..2467)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2656..2889
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2961..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2998..3017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3052..3071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3122..3257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3278..3296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3319..3677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3359..3380)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3571..3590
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3704..3808
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3810..4167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3810..4001
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3833..3853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4018..4153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4202..4996
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4202..4996
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4256..4275)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4866..4885
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5184..5203)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5228..5275
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5604..6192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6093..6112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6367..6670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6672..6870
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6825..6845
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6888..7068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7115..7240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7737..7970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8155..8199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8497..8614
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8686..9864
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8917..9855
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9812..9832
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        9881..9899
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatctggcca atcacaagtt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
       61 ctggatccgg taccgaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac
      121 aaagtggtgg gaggaggtgg atctggcgac tacaaggacg acgatgacaa gtgactcgag
      181 gttaacgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      241 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      301 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      361 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      421 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      481 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      541 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      601 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      661 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc accatgaaaa
      721 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
      781 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      841 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      901 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
      961 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1021 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     1081 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1141 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1201 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1261 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1321 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1381 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1441 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1501 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1561 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1621 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1681 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagt
     1741 gtacaagtag gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt
     1801 tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa
     1861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     1921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     1981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     2341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     2461 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     2521 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     2581 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     2641 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     2701 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2881 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     2941 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3061 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3181 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3241 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattagcat
     3301 gtgagcaaaa ggccagcata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3361 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3421 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3481 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3541 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3601 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3661 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3781 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3841 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3901 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3961 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4081 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4141 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4381 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5161 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5221 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5281 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5341 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5401 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6301 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     6361 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     6421 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     6481 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     6541 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     6601 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     6661 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     6721 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     6781 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     6841 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     6901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     7081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     7141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     7201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     7261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     7321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7561 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     7621 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     7681 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     7741 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     7801 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     7861 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     7921 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     7981 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8041 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8101 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8161 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8221 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8281 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8341 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     8401 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     8461 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8521 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8581 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     8641 tccagtttgg ttagtaccgg gcccgctcta gccctagatt taaatggctc cggtgcccgt
     8701 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     8761 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     8821 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     8881 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     8941 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     9001 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     9061 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     9121 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     9181 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     9241 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     9301 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     9361 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     9421 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     9481 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     9541 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     9601 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     9661 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     9721 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     9781 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     9841 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg
     9901 agacccaagc tggctagccg cggaatctag actgccggat cgaattgtcg acgaattcg
//
