LOCUS       sequence-369338-       17882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of FLAG-HUWE1 delta3291-3298.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17882)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 17882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDEST_FLAG-HUWE1_delta3291-3298
FEATURES             Location/Qualifiers
     source          1..17882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    995..1019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14165..14189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    14223..14845
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14422..14441)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(14869..14889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14874..15203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15054..15189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(15241..15260)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15242..15260)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15274..15290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15274..15290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15287..15309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15298..15314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15322..15328,15329..15346,15347..15352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15367..15388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15505..15522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15676..16264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15756..15775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16435..17226,17227..17295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17058..17077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17296..17400)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      17427..17855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17514..17533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17724..17745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatctgat atcaacaagt ttgtacaaaa aagcaggctt
     1021 taaaggaacc aattcagtcg acatgaaagt agacaggact aaactgaaga agacacctac
     1081 tgaggctcct gcagactgca gagccttaat agacaaactc aaagtttgta atgatgagca
     1141 acttctcttg gaactgcagc agatcaaaac atggaacatt ggaaagtgcg agttatatca
     1201 ctgggtggac ctgttggacc gcttcgatgg aatactggca gatgctggac agacagtgga
     1261 gaatatgtca tggatgctcg tatgtgatag gccagaaaga gagcaactga aaatgcttct
     1321 cttggctgtg ttgaacttca cagccttgct cattgagtac agcttttccc ggcatctgta
     1381 cagttccata gagcatttga caactttatt ggcttcctct gatatgcaag tggtgctggc
     1441 agtcctcaat ctcctatatg tatttagcaa aagatcaaac tacatcactc gtctgggatc
     1501 tgacaagagg accccgctgc taactcggct acaacatttg gcagagagct ggggtggaaa
     1561 ggagaatggc tttggacttg cagaatgttg cagagacttg catatgatga aatatccacc
     1621 cagtgcaact acactacact ttgaattcta tgcagatcct ggggccgagg tcaaaattga
     1681 gaaaaggaca actagtaaca cactacatta tattcacata gagcaacttg acaagatttc
     1741 agaaagccct tctgaaatca tggaatctct taccaaaatg tacagcattc ctaaggataa
     1801 gcagatgctg ttatttacac acatacgact ggcccatggc ttttctaatc acaggaagcg
     1861 attgcaggca gttcaggcca gactgcatgc aatatctata ttagtgtatt ccaatgcctt
     1921 gcaggaatca gcaaacagta tcttgtataa tggcttgata gaggagttag tggacgtgct
     1981 gcaaataacg gataagcagc ttatggagat taaagcagct tctttacgaa cattaacatc
     2041 aattgtccac ttggagagaa ctcccaaact cagcagtatt attgactgta ctggaactgc
     2101 ctcctaccat ggatttttgc cagtgcttgt aaggaactgt atccaggcca tgattgatcc
     2161 ttccatggat ccataccctc accagtttgc cactgctctc ttctcttttt tataccatct
     2221 ggccagctac gatgctggtg gtgaagcctt ggtctcctgt ggaatgatgg aagccttatt
     2281 gaaggtcata aagtttcttg gcgatgaaca ggaccagata acatttgtca ccagagccgt
     2341 cagagtggtt gaccttatca ccaacctgga tatggcagct tttcaatccc atagtggact
     2401 ttctatcttc atttatagac ttgagcatga agtagatctg tgccgaaaag aatgtccgtt
     2461 tgtgatcaag ccaaagatcc agagacccaa tactacacaa gaaggagagg aaatggaaac
     2521 tgatatggat ggagtccagt gtattccaca acgagcagca cttctgaaat ccatgttgaa
     2581 tttcctcaag aaggccatcc aagaccctgc tttctcagat ggcatacgac atgtgatgga
     2641 tggttctctg cctacctccc tgaaacacat catcagcaat gcagaatact atggcccatc
     2701 actcttcctc ctagctactg aagtggtgac tgtgtttgta tttcaagaac catcactgct
     2761 ctcctcactc caggacaatg gattgacaga tgtcatgctg catgcactgc ttatcaaaga
     2821 tgttcctgct acccgtgaag tccttggctc cctcccaaat gtattcagtg cactctgttt
     2881 gaatgcccga ggtcttcagt cttttgttca gtgtcagcct tttgaacgcc tcttcaaagt
     2941 tcttctgtct ccagattacc tcccagccat gcggaggagg agaagttctg atccccttgg
     3001 ggatactgca tccaacctgg ggagtgctgt cgatgagctc atgagacatc agcccaccct
     3061 taaaacagat gcaacgactg ccatcatcaa gttacttgaa gaaatctgta atcttggaag
     3121 ggaccccaaa tacatctgtc agaagccatc gatccagaag gcagatggca ctgccactgc
     3181 tcctccccca aggtctaatc atgccgcaga agaagcctct agtgaggatg aggaggaaga
     3241 ggaagtacag gccatgcaga gctttaattc tacccagcaa aatgaaactg agcctaatca
     3301 gcaggttgtt ggtacagagg aacgtattcc tattcccctc atggattaca tccttaatgt
     3361 gatgaaattt gtggaatcta ttctgagcaa caatacaaca gatgaccact gccaggaatt
     3421 tgtgaatcag aaaggactgt tgcctttggt taccattttg ggtcttccca atctgcccat
     3481 tgactttccc acatctgctg cctgtcaggc tgttgcaggt gtctgcaaat ccatattgac
     3541 actgtcacat gaacccaaag tccttcaaga gggtctcctt cagttggact ccatcctctc
     3601 ctccctggag cccttacacc gccccattga atcccctggg ggctcagtgt tgttgcgaga
     3661 actggcttgc gcaggcaatg ttgctgatgc taccctctca gcccaggcca cacctctgct
     3721 gcatgcactc actgctgccc atgcctacat catgatgttt gttcatactt gcagagttgg
     3781 acagagtgaa attcgttcca tctccgtaaa ccagtggggc tctcaattgg gtctgagtgt
     3841 tttgagcaag ctgagccagt tatactgttc cctggtgtgg gaaagcactg tcctcctctc
     3901 tctgtgtacc ccaaacagcc taccatctgg gtgtgaattt ggccaggcag atatgcagaa
     3961 actggttcca aaggatgaga aggcaggtac gacccagggc ggaaaaagat cagatgggga
     4021 acaggatgga gcagctggaa gtatggatgc ttctacccag ggcttattag aaggcattgg
     4081 gctagatggt gacacattgg ctcccatgga gacagatgaa cctactgctt cagactctaa
     4141 gggcaaatct aaaatcacac cagcaatggc tgccagaatt aagcaaatca agcctttgtt
     4201 atcagcttcc tccagattag gccgagcact tgctgagcta tttggacttc ttgttaaact
     4261 ttgtgtggga tctcctgtcc gccagagaag gagccatcat gctgccagca ccactacagc
     4321 accgacacct gccgcgcgat caacagcctc agctctcact aagctcttga ctaaggggtt
     4381 atcttggcag cccccaccat atacacctac tccccgattc aggctgacat tcttcatctg
     4441 ttcagttggt ttcacatccc caatgctgtt tgatgagagg aagtatccct accacctcat
     4501 gctgcaaaaa tttctctgct ccggaggcca caatgctctt tttgaaactt tcaactgggc
     4561 tctgtccatg ggaggtaaag ttcctgtttc tgagggattg gaacactcag acttgcctga
     4621 tggcacagga gaattcctag atgcctggct tatgctggtg gagaagatgg tgaatcccac
     4681 cacggtgctt gaatctccac attcgctgcc tgccaaattg cctggaggtg tccagaactt
     4741 tccccagttc agtgcactgc gcttccttgt ggtaactcag aaagcagcct ttacttgcat
     4801 caaaaactta tggaaccgga aacccctgaa ggtatatggt ggacgaatgg ctgaatcgat
     4861 gctggccatt ctatgccaca tcctccgagg agaacctgtg attcgagaga gactaagcaa
     4921 ggagaaggag gggtctcgag gagaagagga tacagggcaa gaggaaggtg gctcccgccg
     4981 ggaacctcaa gtcaaccagc aacaactgca acagctcatg gacatgggct tcacaaggga
     5041 acatgcaatg gaggcactgt tgaacaccag caccatggag caggccacag agtacctttt
     5101 aacccaccct cctccaatca tgggaggagt tgttcgggat ctcagcatgt ctgaagagga
     5161 ccagatgatg agagcaattg ctatgtctct gggacaggat attccaatgg atcaaagggc
     5221 agagtcacct gaggaagttg cttgccggaa ggaggaagag gaacggaaag ctcgggaaaa
     5281 gcaggaggag gaagaggcta aatgtctaga gaagttccag gatgctgacc cgttggaaca
     5341 agatgagctc cacactttca cagatactat gttgccaggc tgcttccacc ttcttgatga
     5401 gctgccagac acagtatacc gtgtgtgtga cctgatcatg acagcaatca aacgtaatgg
     5461 agcagattat cgtgacatga ttctgaagca agtagtcaat caggtgtggg aagctgctga
     5521 tgtattgatc aaagctgctc ttcccctgac aacaagtgac acaaaaaccg tgtcagagtg
     5581 gataagtcag atggccacac tgccccaggc ctccaatttg gctactagaa tcttgctttt
     5641 aacgctactt tttgaggagt tgaagctacc ttgtgcttgg gtggttgaat caagtggcat
     5701 ccttaatgtc ctaatcaaac tcttggaagt ggttcagccc tgcctccagg cagccaagga
     5761 gcagaaggaa gtccagaccc caaagtggat cacaccagtg ttgctcctga ttgatttcta
     5821 tgaaaagaca gccatctcct caaaaaggag agcccagatg actaagtacc tgcaatccaa
     5881 cagcaacaac tggcgctggt ttgatgatcg ctctgggcgt tggtgtagtt acagtgcaag
     5941 caacaatagc actattgatt ctgcctggaa atctggagag acaagcgtgc gattcactgc
     6001 aggccgaaga agatacacgg tccaattcac tacaatggtg caggttaatg aggaaacagg
     6061 gaaccgacgc cctgtgatgc tgactctcct cagggtacct cggctgaata aaaattcaaa
     6121 aaacagcaat ggacaggaac tagagaagac gctggaagaa agcaaagaaa tggatatcaa
     6181 acgtaaagaa aataaaggca atgatacccc tttggcccta gagagtacaa acactgaaaa
     6241 ggagacaagc ctggaggaaa caaaaatcgg ggagatcctg atccagggct tgacagaaga
     6301 tatggtgact gttttaatcc gggcctgcgt gagcatgctg ggagtccctg tggacccaga
     6361 tactttgcat gccacccttc gtctctgtct gaggctcacc cgggaccaca aatatgccat
     6421 gatgtttgca gaactgaaga gtacccgcat gatcttgaat ttgacccaga gctcaggctt
     6481 caatgggttt actcccctgg tcacccttct cttaagacac atcattgagg acccctgtac
     6541 ccttcgtcat accatggaaa aggttgttcg ctcagcagct acaagtggag ctggtagcac
     6601 tacctctggt gttgtgtctg gcagcctcgg ctctcgggag atcaactaca tccttcgtgt
     6661 ccttgggcca gccgcatgcc gcaatccaga catattcaca gaagtggcca actgctgtat
     6721 ccgcatcgcc cttcctgccc ctcgaggctc aggaactgct tcagatgatg aatttgagaa
     6781 tcttagaatt aaaggcccta atgctgtaca gctggtgaag accacccctt tgaagccctc
     6841 acctctgcct gtcatccctg atactatcaa ggaagtgatc tatgatatgc tgaatgctct
     6901 ggctgcatac catgctccag aggaagccga taaatctgat cctaaacctg gggttatgac
     6961 ccaagaggtt ggccagctcc tgcaagacat gggtgatgat gtataccagc agtaccggtc
     7021 acttacgcgt cagagcagtg actttgatac gcagtcaggt ttttccatta atagtcaggt
     7081 ctttgctgca gatggtgcct ccactgagac ttccgcatct gggacctccc aaggagaggc
     7141 ttcaactcca gaggagtctc gagatgggaa gaaagataaa gaaggggacc gggcctctga
     7201 ggaaggcaaa cagaaaggca agggcagcaa acctttaatg cctacctcca ctatccttcg
     7261 tcttctggca gagttggtga ggtcctatgt tggtattgct accctgattg ccaactacag
     7321 ctacactgtg ggccagtctg aactgatcaa agaggactgc agtgtgctag cttttgttct
     7381 ggaccacctg ctcccacata cccagaatgc agaagacaag gacacccctg ccttggcccg
     7441 cctgttcctc gcaagcctgg ctgctgcagg gagtggcaca gatgcccagg tggccctagt
     7501 gaatgaagta aaagcagccc ttggacgggc actggctatg gctgagagta cagagaaaca
     7561 tgccaggctt caggcagtga tgtgtatcat cagtactatc atggagtcct gcccctccac
     7621 ctccagcttc tacagcagtg ccacagcgaa gacccagcac aatggcatga acaacatcat
     7681 tcggcttttc ctgaagaagg gactggttaa tgacctggcc agagtacctc acagcttaga
     7741 cctgtccagt cccaacatgg ccaacacagt caatgctgct ctgaagcctt tggaaacact
     7801 ttcccggatt gtgaaccagc ccagtagcct ttttggcagc aagagtgctt ctagcaagaa
     7861 caagtctgag caggatgccc aaggagcctc tcaagattcc agtagcaacc agcaggaccc
     7921 aggcgagcct ggggaagcag aagtgcagga ggaggatcat gatgtcactc agacagaggt
     7981 ggcagatggg gatatcatgg atggggaggc tgaaaccgac tcagtggtga ttgctgggca
     8041 gcctgaggtg ctcagttcac aagagatgca ggttgagaat gagctggagg acctgataga
     8101 tgagttgctt gagagggatg gcggatctgg gaacagtaca attatagtga gcagaagtgg
     8161 agaggatgaa tcacaagagg acgtgctgat ggatgaagct ccttccaacc tcagccaagc
     8221 ttccaccttg caggccaacc gagaagattc catgaatatc ctggaccctg aggatgagga
     8281 ggagcacact caggaagagg acagcagtgg cagtaacgag gatgaggatg atagtcagga
     8341 tgaagaggag gaggaggagg aagatgagga agatgatcag gaggatgatg aaggtgaaga
     8401 gggagatgaa gacgatgacg acgatggctc tgagatggaa ttggatgagg attatcctga
     8461 tatgaacgct tctcccttgg tccgatttga gcgctttgac cgggaggatg atctcatcat
     8521 tgagtttgac aacatgttct ccagtgctac agacatcccc ccatccccag gaaatatccc
     8581 taccacccat ccactgatgg tgcgccatgc agaccacagt tctctgacac tgggcagtgg
     8641 ctcttcaaca actcgtctca cccagggcat cgggcgcagt cagaggaccc taaggcagct
     8701 gacggccaat actggccaca ccattcatgt tcactaccct gggaatcgcc agcccaaccc
     8761 tcctcttata ctgcagaggt tgcttggtcc ctcagctgct gctgacatcc ttcagctgag
     8821 cagcagcctt cccctacaaa gccggggtcg ggcccgcctc ctggtaggca acgatgacgt
     8881 ccacatcatc gcccgttctg atgatgagct gctggatgac tttttccatg atcagagcac
     8941 agctaccagc caagcaggaa ccctgtccag catccccaca gccctgaccc gctggacaga
     9001 agaatgcaaa gttctcgatg ctgagagcat gcatgactgt gtttcagtgg ttaaagtgtc
     9061 cattgtcaat cacctggaat tcctgaggga tgaggagctg gaagaaaggc gagagaagcg
     9121 caggaaacaa ctggctgagg aagaaacaaa gataactgat aaaggcaaag aagataagga
     9181 gaacagggat cagagtgcac agtgtactgc atctaagtca aatgactcca ctgaacagaa
     9241 tctctcagat gggacgccta tgcctgacag ctacccaaca accccatctt caactgatgc
     9301 agctacatct gagtccaagg agacccttgg cactctgcaa tcctcacaac agcaaccaac
     9361 actcccaacc ccaccagctt tgggagaggt tcctcaggag ctgcagtctc cagctggaga
     9421 agggggcagc tctacacagc tattgatgcc tgtagagcca gaggaattgg gtcccacaag
     9481 gccaagtggg gaagcagaaa caactcagat ggagttatcc ccagctccca ctataacctc
     9541 actttcccca gagagagctg aggattctga tgcactgacg gctgtcagca gtcagctaga
     9601 aggctctcct atggatacaa gcagcctggc ttcctgtacc ttagaggagg ctgtgggtga
     9661 cacttcagca gctggcagtt ctgagcagcc cagagcaggc agctccactc ctggggatgc
     9721 cccaccagct gtggcggaag tgcaaggcag gagtgatggg tcaggggaat ctgcccagcc
     9781 acctgaggac agctccccac ctgcatcctc tgagagctct tccaccagag attctgccgt
     9841 ggccatttct ggcgccgatt ctcggggaat actggaagag ccactgccat ctacctctag
     9901 tgaggaagaa gatcctcttg ccggtatatc attgcccgag ggggtagacc cgtctttcct
     9961 cgccgccctc ccggacgata ttcggagaga agtacttcaa aatcaactcg gaattcggcc
    10021 ccccactcgc actgccccga gtactaatag ctcagcgccc gccgttgtcg ggaatccagg
    10081 agtcactgaa gtgagtccgg agttccttgc agcactcccg ccggctattc aggaagaagt
    10141 ccttgcgcaa cagcgagcgg aacaacagag gcgagaactt gcccaaaacg catcttcaga
    10201 cactccaatg gaccctgtca cgttcataca gacccttccc tccgacttgc gacgatccgt
    10261 gctggaagac atggaagatt ctgtacttgc cgtaatgccg cctgatattg cggcagaagc
    10321 acaagctctt cgaagggaac aagaagcaag gcagcgacag ttgatgcatg agaggctctt
    10381 cggtcactcc tcaacctccg cgttgtctgc catactcaga tcacccgcgt tcacaagccg
    10441 cctctcagga aatcggggcg tccagtacac cagattggcc gtccaacgag gcggcacgtt
    10501 tcaaatgggc gggtctagct cacataatag gccaagcggc tcaaatgtag atactctgct
    10561 gcgacttcgg ggacgactgc tgttggatca cgaagcgctc tcctgtctct tggttctgct
    10621 cttcgttgat gagcctaagc tcaacacaag taggctccat cgcgtactgc ggaatttgtg
    10681 ctatcatgcg cagacacgcc attgggtaat taggagcttg ctttcaatcc tgcagcgaag
    10741 ttcagaatcc gaactttgca tagaaacacc aaagcttacc acttccgagg aaaaggggaa
    10801 aaagtcttca aaatcttgcg gtagcagctc acatgaaaat cgcccgctcg atcttttgca
    10861 caaaatggaa agcaagtcta gtaatcagct ctcctggctt tccgtttcca tgggatctgg
    10921 gaatatattt cagattcaga gatctggtgg ccgcaagcat acagaaaagc atgcttctgg
    10981 tggaagtacg gtacacatac accctcaggc agcccctgtc gtatgcaggc acgtgttgga
    11041 cacactcatc cagctcgcta aggtctttcc ctcccatttt actcagcaac gaacgaagga
    11101 aaccaattgc gagagtgacc gcgaaagagg gaacaaagcg tgctctccgt gttcttccca
    11161 gtcttcaagt agcggcattt gtactgattt ttgggatctg cttgtgaaac tggataacat
    11221 gaacgtttcc agaaagggga aaaactccgt caaatctgtc ccggtgtctg cgggaggcga
    11281 gggtgagaca tccccttact ctctggaggc atcacccctg ggacaactta tgaatatgct
    11341 cagccaccct gtgatacgaa gatcctcact tctgacggaa aagctgctca ggctgctttc
    11401 actgatttcc atagcacttc ctgagaacaa ggtgagtgag gcacaggcaa attccgggtc
    11461 tggagcttct tccacaacga ctgcgacctc cacaaccagc acaacaacta cgactgctgc
    11521 gtctaccacc cctactcccc ctacggcgcc tactcccgtc acatccgcgc cggccctcgt
    11581 agccgctacg gcgataagta cgatcgtcgt tgcggccagt actacggtca ccacaccgac
    11641 cacagcgacc acgacggtca gtattagtcc tacgactaag gggtcaaaga gccctgctaa
    11701 agtgtccgac ggaggatcct cctcaacaga ttttaaaatg gtgtcctctg gacttacgga
    11761 aaaccagctg cagctttccg tagaggtact tacctcacat tcctgcagcg aggaaggact
    11821 cgaagacgct gcgaatgtgc tcctgcagct gagtagagga gattccggta ccagggatac
    11881 cgttctgaaa ttgcttctta atggcgccag gcacctcggg tacactctgt gcaagcaaat
    11941 tggtactctg ttggccgaac tccgagaata caatctggag cagcaacgac gggcgcagtg
    12001 tgaaacactg agtcccgacg ggcttcctga ggaacagccg cagacaacga aacttaaggg
    12061 taaaatgcag agtcggttcg acatggcaga aaatgtcgtg atagtggctt ctcagaaacg
    12121 ccctctcggg ggaagggaac tgcagctgcc cagtatgtcc atgcttactt ccaagacaag
    12181 cacccagaag ttttttttga gagtacttca agtcataatt caattgagag acgatactcg
    12241 ccgagccaac aagaaagcca aacaaacggg aaggctcggt tcttctggtc tgggctcagc
    12301 atcatccatc caggcggccg tgcggcaact tgaagcagag gcagacgcca tcatacaaat
    12361 ggtacgtgag ggtcaaaggg cgcggagaca gcaacaagca gcaacgtcgg agtctagcca
    12421 gtcagaggcg tctgtccgga gggaggaatc acccatggat gtggaccagc catctcccag
    12481 tgctcaagat actcaatcca ttgcctccga tggaacccca cagggggaga aggaaaagga
    12541 agaaagacca cctgagttac ccctgctcag cgagcagctg agtttggacg agctgtggga
    12601 catgcttggg gagtgtctaa aggaactaga ggaatcccat gaccagcatg cggtgctagt
    12661 gctacagcct gctgtcgagg ccttctttct ggtccatgcc acagagcggg agagcaagcc
    12721 tcctgtccga gacacccgtg agagccagct ggcacacatc aaggacgagc ctcctccact
    12781 ctcccctgcc cccttaaccc cagccacgcc ttcctccctt gacccattct tctcccggga
    12841 gccctcatct atgcacatct cctcaagcct gccccctgac acacagaagt tccttcgctt
    12901 tgcagagact caccgcactg tgttaaacca gatcctacgg cagtccacga cccaccttgc
    12961 tgatgggcct tttgctgtcc tggtagacta cattcgtgtc ctcgactttg atgtcaagcg
    13021 caaatatttc cgccaagagc tggagcgttt agatgagggg ctccggaaag aagacatggc
    13081 tgtgcatgtc cgtcgtgacc atgtgtttga agactcctat cgtgagctgc atcgcaaatc
    13141 ccccgaagaa atgaagaatc gattgtatat agtatttgaa ggagaagaag ggcaggatgc
    13201 tggcgggctc ctgcgggagt ggtatatgat catctctcga gagatgttta accctatgta
    13261 tgccttgttc cgtacctcac ctggtgatcg agtcacctac accatcaatc catcttccca
    13321 ctgcaacccc aaccatctgt cctattttaa atttgtcgga cgcattgtgg ccaaagctgt
    13381 atatgacaac cgtcttctgg agtgctactt tactcgatcc ttttacaaac acatcttggg
    13441 caagtcagtc agatatacag atatggagag tgaagattac cacttctacc aaggtctggt
    13501 ttatctgctg gaaaatgatg tctccacact aggctatgac ctcaccttca gcactgaggt
    13561 ccaagagttt ggagtttgtg aagttcgtga cctcaaaccc aatggggcca acatcttggt
    13621 aacagaggag aataagaagg agtatgtaca cctggtatgc cagatgagaa tgacaggagc
    13681 catccgcaag cagttggcgg ctttcttaga aggcttctat gagatcattc caaagcgcct
    13741 catttccatc ttcactgagc aggagttaga gctgcttata tcaggactgc ccaccattga
    13801 catcgatgat ctgaaatcca acactgaata ccacaagtac cagtccaact ctattcagat
    13861 ccagtggttc tggagagcat tgcgttcttt cgatcaagct gaccgtgcca agttcctcca
    13921 gtttgtcacg ggtacttcca aggtacccct gcaaggcttt gctgccctcg aaggcatgaa
    13981 tggcattcag aagtttcaga tccatcgaga tgataggtcc acagatcgcc tgccttcagc
    14041 tcacacatgt tttaatcagc tggatctgcc tgcctatgag agctttgaga agctccgcca
    14101 catgctactg ttggctatcc aggagtgctc tgaaggcttt gggctggcct aataagcggc
    14161 cgcgacccag ctttcttgta caaagtggtt gatatcggta ccagtcgact ctagaggatc
    14221 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
    14281 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
    14341 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
    14401 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
    14461 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
    14521 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
    14581 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
    14641 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
    14701 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
    14761 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
    14821 ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc
    14881 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    14941 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    15001 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    15061 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    15121 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    15181 ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt
    15241 ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
    15301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    15361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    15421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    15481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    15541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    15601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    15661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    15721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    15781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    15841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    15901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    15961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    16021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    16081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    16141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    16201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    16261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    16321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    16381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    16441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    16501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    16561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    16621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    16681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    16741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    16801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    16861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    16921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    16981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    17041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    17101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    17161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    17221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    17281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    17341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    17401 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
    17461 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    17521 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    17581 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    17641 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    17701 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    17761 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    17821 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    17881 tt
//
