LOCUS       sequence-36934-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3904 (=HL 770 + pHL 991)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(63..80)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      233..319
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      411..438
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    505..523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    530..548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    789..805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tggatgagct ctacaaataa aagctagctt ggctgttttg gcggatgaga gaagattttc
       61 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
      121 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
      181 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
      241 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      301 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
      361 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
      421 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
      481 caaatatgta tccgctcagg atcctcccta tcagtgatag agattgacat ccctatcagt
      541 gatagagata ctcccggggc gatcaggaag accctcgcgg agaacctgaa agcacgacat
      601 tgctcacatt gcttccagta ttacttagcc agccgggtgc tggctttttt tttgatcttt
      661 cgttctcaat ttatccacaa gctttaatcg tacagggtag tacaaataaa aaaggcacgt
      721 cagatgacgt gccttttttc ttgtgagcag tgaattcgat ccataaatgt gagcggataa
      781 cattgacatt gtgagcggat aacaagatac t
//
