LOCUS       sequence-369354-        9513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  optimisation of phosphinothricin (Basta) selection in plant cells; 
            combines kanamycin resistance gene (nptII) and Basta resistance gene
            (bar) with fluorescent marker (eGFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Kobercova E, Srba M, Fischer L
  TITLE     Sulfadiazine and phosphinothricin selection systems optimised for 
            the transformation of tobacco BY-2 cells.
  JOURNAL   Plant Cell Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7.
  PUBMED    36609768
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. 2023 Jan 7. doi: 10.1007/s00299-022-02975-7."
COMMENT     Sequence Label: pDGB1alpha2_KanR_BastaR_Rb7_GFP
FEATURES             Location/Qualifiers
     source          1..9513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(49..301)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(341..1135)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(452..471)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1062..1081
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1169..1348)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1853..2197
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2349..2897
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(3467..3484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3467..3483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3476..3498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        4982..5027
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(5661..5663,5664..5666,5667..6380)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5706..5727)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5967..5986)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6314..6335
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6391..6408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6415..6432
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6683..6935
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6952..6976
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7067..7655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7147..7166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7826..8641)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7826..8641)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8549..8568
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8633..8652
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8932..9367
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    join(9494..9513,1..3)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 taacaagctt cgtctcagtc aggagagcga tcagcttgca tgccggtcga tctagtaaca
       61 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
      121 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
      181 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
      241 tggcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
      301 ctgcttgact ctagctagag tccgaacccc agagtcccgc tcagaagaac tcgtcaagaa
      361 ggcgatagaa ggctatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
      421 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
      481 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
      541 ccaccatgat attcggcaag caggcgtcgc cgtgggtcac gacgagatcc tcgccgtcgg
      601 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
      661 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcggt
      721 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
      781 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
      841 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
      901 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
      961 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     1021 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     1081 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcctc
     1141 gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
     1201 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
     1261 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
     1321 ctcagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
     1381 cgtcatcggc gggggtcata ccgtgactcc cttaattctc atgtatctcc gtcaggagat
     1441 gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     1501 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     1561 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     1621 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     1681 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1741 ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac
     1801 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
     1861 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
     1921 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
     1981 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     2041 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     2101 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     2161 cctctatata aggaagttca tttcatttgg agaggacctc gagaattaat tctcaacaca
     2221 acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt gcagcaattt
     2281 aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt acgaacgggg
     2341 gatctaccat gagcccagaa cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca
     2401 tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc aacttccgta
     2461 ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc
     2521 cctggctcgt cgccgaggtg gacggcgagg tcgccggcat agcctacgcg ggcccctgga
     2581 aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtatcc ccccgccacc
     2641 agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg gaggcacagg
     2701 gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg
     2761 aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag cacgggaact
     2821 ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc cgtccggtcc
     2881 tgcccgtcac cgagatctgc tcaacaatct agctagagtt tgctcctatc tatatgtaat
     2941 aaggtatgct gatatgcact attcaaatag gagcattagc tatgtttgtt aatgtcactt
     3001 tatgttatgt gggtaagtca cctaagacac tccacgtacc tacttgttgt ctcttacgcg
     3061 gctttaataa atcttctgcc cttgttccat atttactaat tatccctttc ttcactaaaa
     3121 gaaaattgtt atcattaagt attagtcttt agaacatatg aggtctttaa ttgggtaggt
     3181 tttacaaatt aactaatata aaatgtcata aaatccacgt ggttaaacaa atgcagaaaa
     3241 tcgacgtcgt ctattggacc gacagttgct attaatataa tgggccacca tagtagactg
     3301 acaaataaat tacctgacaa catcgtttca caaaaaaaca aacacaaaaa gggagtgcat
     3361 tttccagggc atttttgtaa taaaaaacag ttaaaaggga gtgcaataga aatatagggg
     3421 tgtggaaata gtgatttgag cacgtcttga agcgaattag cttggcactg gccgtcgttt
     3481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact cgctgtcagg agtcgattaa
     3541 aaatcccaat tatatttggt ctaatttagt ttggtattga gtaaaacaaa ttcgaaccaa
     3601 accaaaatat aaatatatag tttttatata tatgccttta agacttttta tagaattttc
     3661 tttaaaaaat atctagaaat atttgcgact cttctggcat gtaatatttc gttaaatatg
     3721 aagtgctcca tttttattaa ctttaaataa ttggttgtac gatcactttc ttatcaagtg
     3781 ttactaaaat gcgtcaatct ctttgttctt ccatattcat atgtcaaaat ctatcaaaat
     3841 tcttatatat ctttttcgaa tttgaagtga aatttcgata atttaaaatt aaatagaaca
     3901 tatcattatt taggtatcat attgattttt atacttaatt actaaatttg gttaactttg
     3961 aaagtgtaca tcaacgaaaa attagtcaaa cgactaaaat aaataaatat catgtgttat
     4021 taagaaaatt ctcctataag aatattttaa tagatcatat gtttgtaaaa aaaattaatt
     4081 tttactaaca catatattta cttatcaaaa atttgacaaa gtaagattaa aataatattc
     4141 atctaacaaa aaaaaaacca gaaaatgctg aaaacccggc aaaaccgaac caatccaaac
     4201 cgatatagtt ggtttggttt gattttgata taaaccgaac caactcggtc catttgcacc
     4261 cctaatcata atagctttaa tatttcaaga tattattaag ttaacgttgt caatatcctg
     4321 gaaattttgc aaaatgaatc aagcctatat ggctgtaata tgaatttaaa agcagctcga
     4381 tgtggtggta atatgtaatt tacttgattc taaaaaaata tcccaagtat taataatttc
     4441 tgctaggaag aaggttagct acgatttaca gcaaagccag aatacaaaga accataaagt
     4501 gattgaagct cgaaatatac gaaggaacaa atatttttaa aaaaatacgc aatgacttgg
     4561 aacaaaagaa agtgatatat tttttgttct taaacaagca tcccctctaa agaatggcag
     4621 ttttcctttg catgtaacta ttatgctccc ttcgttacaa aaattttgga ctactattgg
     4681 gaacttcttc tgaaaatagt cgctgtcagg agggaaacct cctcggattc cattgcccag
     4741 ctatctgtca ctttattgag aagatagtgg aaaaggaagg tggctcctac aaatgccatc
     4801 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
     4861 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     4921 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     4981 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggtat taaaatctta
     5041 ataggttttg ataaaagcga acgtggggaa acccgaacca aaccttcttc taaactctct
     5101 ctcatctctc ttaaagcaaa cttctctctt gtctttcttg cgtgagcgat cttcaacgtt
     5161 gtcagatcgt gcttcggcac cagtacaacg ttttctttca ctgaagcgaa atcaaagatc
     5221 tctttgtgga cacgtagtgc ggcgccatta aataacgtgt acttgtccta ttcttgtcgg
     5281 tgtggtcttg ggaaaagaaa gcttgctgga ggctgctgtt cagccccata cattacttgt
     5341 tacgattctg ctgactttcg gcgggtgcaa tatctctact tctgcttgac gaggtattgt
     5401 tgcctgtact tctttcttct tcttcttgct gattggttct ataagaaatc tagtattttc
     5461 tttgaaacag agttttcccg tggttttcga acttggagaa agattgttaa gcttctgtat
     5521 attctgccca aattcgcgac cggtccatag gtacttcatg cttcaacgtg taacttaaga
     5581 gatactgtgt gaaattttat atttccatac atttgcttga cctttgcttt ttgtcaattt
     5641 ttttcccctt acaggtaaaa atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5701 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5761 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5821 tgcccgtgcc ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc
     5881 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5941 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6001 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6061 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6121 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6181 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6241 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     6301 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     6361 tggacgagct gtacaagtaa gcttcccggg catcaccatc accatcatcc cgggcatcac
     6421 catcaccatc actagctcga ggcctttaac tctggtttca ttaaattttc tttagtttga
     6481 atttactgtt attcggtgtg catttctatg tttggtgagc ggttttctgt gctcagagtg
     6541 tgtttatttt atgtaattta atttctttgt gagctcctgt ttagcaggtc gtcccttcag
     6601 caaggacaca aaaagatttt aattttatta aaaaaaaaaa aaaaaaagac cgggaattcg
     6661 atatcaagct tatcgacctg cagatcgttc aaacatttgg caataaagtt tcttaagatt
     6721 gaatcctgtt gccggtcttg cgacgattat catataattt ctgttgaatt acgttaagca
     6781 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     6841 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     6901 attatcgcgc gcggtgtcat ctatgttact agatccgctt gagacgaagc ttgacaggat
     6961 atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag
     7021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag
     7561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7801 tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa tgaaactgca
     7861 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     7921 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     7981 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     8041 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     8101 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     8161 ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa
     8221 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     8281 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     8341 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     8401 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     8461 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt
     8521 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     8581 catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca
     8641 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     8701 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     8761 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
     8821 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
     8881 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc cccatccaac
     8941 agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg gtagttatcc
     9001 acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg cccgctccaa
     9061 aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa
     9121 atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc
     9181 ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca gcaagtagta
     9241 tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc cttggcttgt
     9301 ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt
     9361 cgagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat aaattttttt
     9421 ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat ttccgatccc
     9481 cggaattaga tcttggcagg atatattgtg gtg
//
