LOCUS       sequence-369355-        2670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis
            MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT018
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(38..626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(118..137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             808..1467
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(874..893)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1538..1572
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1946..1966)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
       61 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gattgatcgg gcacgtaaga
      721 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      781 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      841 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      901 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      961 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     1021 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     1081 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     1141 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     1201 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tttcagccaa tccctgggtg
     1261 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1321 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1381 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1441 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
     1501 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     1561 ggcgtttttt attggtctgg tctcaggatc cttctttctt tggatttggt ggtgtatctt
     1621 catcatcgaa caagtagttg tagacgtcat ccatagtagt agtgttaaac ataccggtgg
     1681 taataccgaa agcattataa gcagtttgat cagtccaacc tggagacaat ggcttagaat
     1741 tgttaccatc atcaattttg gaggcggtga tttcgttgtt catgaagtta tcgtgaacat
     1801 tagaagaggc aattggctga gacaaagctt gagaattagt ttgggtagtg atcaagtttg
     1861 gaccgttgga agaattagtg aaagtgaaag acaaagagga gtcggcaata ttaccagact
     1921 gattgaaatt agcgctaccg gaaccaactt ttctcttttt cttaggaggt gaaccactac
     1981 cagattcgca ttttagctgc ttttccaagc cgcaaataat caactctaaa ccgaacaaga
     2041 atgcaggttc tgcaccctgt ctatcaaaca gttcgatagc ttgtcttagc aatggaggca
     2101 ttgaatctgt tgtaggtgtt tctctctctt ctttagcaac ctggtgctct tgttcttcta
     2161 gtacgcaacc taaagtgaag tgaccaactg cacttaaagc gtataaggca ttctccaatg
     2221 aaaaaccctg ttggcataaa aaggctagct gattctccaa agtctcatac tgtttttctg
     2281 ttggccttgt acctagatga accttggcac catcacgatg gctcaacaag gcgcatctaa
     2341 aggatttagc attgttacgc aaaaagtctt gccaactctc accctctaat gggcaaaaat
     2401 gagtgtgatg acgatctaac atttcgattg gcaatgcgtc tagcaatgct ctcttgtttt
     2461 tgacgtgcca atataatgtt ggctgctcta cacctaactt ttgtgctaat tttctggtcg
     2521 ttaagccttc tatacctaca ccgttcaaca gttccaaggc accgttaata accttgcttt
     2581 tgtccaatct tgacatatga gaccgactac ggttatccac agaatcaggg gataacgcag
     2641 gaaagaacat gtgagcaaaa ggccagcaaa
//
