LOCUS       sequence-369356-        2361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mScarlet-I as a Type 3 part to be used in the Dueber YTK and
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT019
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(169..757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(249..268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             939..1598
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1005..1024)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1669..1703
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 catgtgtacc ttgaatctca taaattcctt aataactgct tcacctttag aaaccatatg
       61 agaccgacta cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgattgatcg
      841 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      901 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      961 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1021 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1081 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1141 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1201 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1261 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1321 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtttcagcca
     1381 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1441 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1501 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1561 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     1621 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1681 ggttgccgcc gggcgttttt tattggtctg gtctcaggat cccttgtata attcgtccat
     1741 accaccagtt gagtgtctac cttcacttct ttcatattgt tcaacaactg tgtaatcttc
     1801 attatgagaa gtgatatcca actttctatc aacgttgtaa gcacctggca tttgaactgg
     1861 tttcttagcc ttgtatgtag tcttgaaatc agccaaatat ctaccaccat ccttcaatct
     1921 caatgccatc ttgatgtcac ccttcaaaac accatcttct gggtacaatc tttctgttga
     1981 agcttcccaa cccatagttt tcttttgcat aactggacca tctggtggaa aattagtacc
     2041 tctcaactta accttataga tcaatgtacc atcttccaaa gaagtatctt gtgtaacagt
     2101 aactgcacca ccatcttcga agttcataac tctttcccat ttaaaacctt ctgggaatga
     2161 ttgcttgtag taatctggga tatctgctgg atgtttaata aaagctcttg aaccgtacat
     2221 gaattgtgga gacaagatat cccaagaaaa tggcaatgga ccacctttag taaccttcaa
     2281 cttagcagtt tgagtacctt cgtatggtct accttcacct tcaccttcaa tttcaaattc
     2341 gtgaccgttc atactacctt c
//
