LOCUS       sequence-369357-        2368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mScarlet-I as a Type 4a part to be used in the Dueber YTK 
            and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT022
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(434..1022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(514..533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1204..1863
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1270..1289)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1934..1968
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cagtaactgc accaccatct tcgaagttca taactctttc ccatttaaaa ccttctggga
       61 atgattgctt gtagtaatct gggatatctg ctggatgttt aataaaagct cttgaaccgt
      121 acatgaattg tggagacaag atatcccaag aaaatggcaa tggaccacct ttagtaacct
      181 tcaacttagc agtttgagta ccttcgtatg gtctaccttc accttcacct tcaatttcaa
      241 attcgtgacc gttcatacta ccttccatgt gtaccttgaa tctcataaat tccttaataa
      301 ctgcttcacc tttagaaacg gattgagacc gactacggtt atccacagaa tcaggggata
      361 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      841 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1021 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1081 aagggatttt ggtcatgatt gatcgggcac gtaagaggtt ccaactttca ccataatgaa
     1141 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1201 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1261 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1321 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1381 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     1441 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1501 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1561 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1621 gagaatatgt ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1681 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1741 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     1801 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     1861 taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact
     1921 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtctggtctc
     1981 agccactcga gttacttgta taattcgtcc ataccaccag ttgagtgtct accttcactt
     2041 ctttcatatt gttcaacaac tgtgtaatct tcattatgag aagtgatatc caactttcta
     2101 tcaacgttgt aagcacctgg catttgaact ggtttcttag ccttgtatgt agtcttgaaa
     2161 tcagccaaat atctaccacc atccttcaat ctcaatgcca tcttgatgtc acccttcaaa
     2221 acaccatctt ctgggtacaa tctttctgtt gaagcttccc aacccatagt tttcttttgc
     2281 ataactggac catctggtgg aaaattagta cctctcaact taaccttata gatcaatgta
     2341 ccatcttcca aagaagtatc ttgtgtaa
//
