LOCUS       sequence-369360-        2380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mNeonGreen as a Type 4a part to be used in the Dueber YTK 
            and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT023
FEATURES             Location/Qualifiers
     source          1..2380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(624..1212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(704..723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1394..2053
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1460..1479)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2124..2158
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtagtgtaag accacttgaa agtagagatg atggtcttat cgtttgggta agtttttttg
       61 gatctgcacc aatcagcagc agtcaaagaa ttagtcataa ctggaccatc agctggaaaa
      121 cctgtacctt ttacttgagc ttcaccctta atatgggaac cttcgtaagt atatctgtag
      181 ttgacggtca aagaagcacc atcttcaaat tgcatggttc tatgaacttg gtaaccagaa
      241 ccatcaacca tagcagcttg aaatggagac ataccatctg gatatggcaa atattggtgg
      301 aaaccgtaac caatatgtgg aaccaaaatc catggggaga attgcaagtc accttttgta
      361 gatttcaggt tcaactcttc gtaaccgtcg tttggattac cagtaccttg accaaccata
      421 tcgaaatcaa caccgttgat ggaaccgaaa atatgcaatt cgtgagtagc tggcaaagaa
      481 gccatgttat cctcttcacc tttagacacg gattgagacc gactacggtt atccacagaa
      541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1021 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1261 aactcacgtt aagggatttt ggtcatgatt gatcgggcac gtaagaggtt ccaactttca
     1321 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     1381 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1441 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1501 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     1561 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
     1621 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1681 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     1741 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1801 agggtttatt gagaatatgt ttttcgtttc agccaatccc tgggtgagtt tcaccagttt
     1861 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     1921 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     1981 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     2041 gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga
     2101 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     2161 gtctggtctc agccactcga gttacttgta caactcatcc atacccataa catcggtgaa
     2221 agccttttgc cattctttga agttcaactc ggttttggag tgtttcaatt cggtctttct
     2281 gaacacgtac ataggttgat tcttcaagta gttagcagcc ataggtttag cgaaggtgta
     2341 agtagttcta gcagtacttc tatatctctt accgttacca
//
