LOCUS       sequence-369361-        2383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mTagBFP2 as a Type 4a part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT024
FEATURES             Location/Qualifiers
     source          1..2383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(521..1109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(601..620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1291..1950
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1357..1376)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2021..2055
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taactggacc gttagaagtg aagttgacac ctctaatctt aacgttgtag atcaagcaac
       61 cgtcttgtaa agaagtatct tgagtagcag tcaaaacacc accatcttcg taagtagtaa
      121 ctctttccca agtgaaacct tctggaaaag attgcttgaa gaaatctggg ataccttgag
      181 tgtggttgat gaaagtctta gaaccgtaca agaaggaggt agccaaaata tcaaaagcaa
      241 atggcaatgg accaccttca acaaccttga ttctcatggt ttgagtacct tcgtatggtt
      301 taccttcacc ttcagaggta cacttgaaat gatggttatc aacagtacct tccatgtaca
      361 acttcatgtg catgttttct ttgatcaact cttcaccctt agacacggat tgagaccgac
      421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgattgat cgggcacgta
     1201 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
     1261 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     1321 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     1381 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     1441 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     1501 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     1561 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     1621 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     1681 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtttcagc caatccctgg
     1741 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     1801 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     1861 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     1921 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
     1981 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     2041 ccgggcgttt tttattggtc tggtctcagc cactcgagtt agttcaactt gtgacccaac
     2101 ttagatggca aatcacaata tctagcaaca gcaacttcgt gttgttcaac gtaggtttcg
     2161 ttgttggctt ctttgattct ttccaatcta tagtcgacgt agtaaacacc tggcattttc
     2221 aaattcttag ctggcttctt agacctgtag gtagttttag cattagcaat caaatgggag
     2281 ccaccaacca atttcaaagc catatcattt ctaccttcca aaccaccatc agctggatat
     2341 aatgtttcgg tgaaagcttc ccaacctaag gtcttttttt gca
//
