LOCUS       sequence-369365-        1900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tTDH2 as a Type 4 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT027
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(388..976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(468..487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1158..1817
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1224..1243)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      join(1888..1900,1..22)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gttgccgccg ggcgtttttt attggtctgg tctcacagca aaacggtcga atgtaagcat
       61 atatctttcg attgtataat tgttcccaaa tactacagca tctcaaggaa aaaaaaacaa
      121 aaacttccaa aaaaatcgaa tccctgagga atctttaata cattttcaat ctatttaagt
      181 tttataaacg tgtatatgag atgtcatgag catgaattat taataataaa aactaaatca
      241 ttaaagtaac ttaaggagtt aaatctcgag ttaggattga gaccgactac ggttatccac
      301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      361 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1021 cgaaaactca cgttaaggga ttttggtcat gattgatcgg gcacgtaaga ggttccaact
     1081 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1141 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1201 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1261 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1321 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     1381 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1441 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1501 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1561 ctaaagggtt tattgagaat atgtttttcg tttcagccaa tccctgggtg agtttcacca
     1621 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1681 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1741 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1801 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
     1861 atgacctcag aactccatct ggatttgttc agaacgctcg
//
