LOCUS       sequence-369366-        1900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes tPDC1 as a Type 4 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT028
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(59..647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(139..158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             829..1488
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(895..914)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1559..1593
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
       61 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      121 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      181 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      241 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      301 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      361 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      421 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      481 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      541 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      601 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      721 tgattgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      781 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      841 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      901 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      961 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1021 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
     1081 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1141 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1201 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1261 gtttcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1321 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1381 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1441 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag
     1501 ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
     1561 cagaacgctc ggttgccgcc gggcgttttt tattggtctg gtctcacagc ttttccaacg
     1621 aaaagaatat acatatcgtt taagagaaat tctccaaatt tgtaaagaag cggacccaga
     1681 cttaagccta accaggccaa ttcaacagac tgtcggcaac ttcttgtctg gtctttccat
     1741 ggtaagtgac agtgcagtaa taatatgaac caatttattt ttcgttacat aaaaatgctt
     1801 ataaaacttt aactaataat tagagattaa atcgcctcga gttaggattg agaccgacta
     1861 cggttatcca cagaatcagg ggataacgca ggaaagaaca
//
