LOCUS       sequence-369367-        2721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes URA3 as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT029
FEATURES             Location/Qualifiers
     source          1..2721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(231..1034)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1323..1911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1403..1422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2093..2721,1..31)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2159..2178)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt gaagacatac tcgggtaata actgatataa ttaaattgaa
      181 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
      241 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
      301 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
      361 gaaaccacat catccacggt tctatactgt tgacccaatg catctccctt gtcatctaaa
      421 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
      481 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
      541 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
      601 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
      661 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
      721 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcctcgaa gagtaaaaaa
      781 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
      841 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
      901 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
      961 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     1021 gtagctttcg acatgattta tcttcgtttc ctgcacgttt ttgttctgtg cagttgggtt
     1081 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     1141 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     1201 caaggaaacc gtgtaatgtc ttcacggtta tccacagaat caggggataa cgcaggaaag
     1261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1981 gtcatgattg atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact
     2041 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     2101 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     2161 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     2221 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     2281 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
     2341 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2401 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2461 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2521 tttcgtttca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2581 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2641 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2701 aatgcttaat gaattacaac a
//
