LOCUS       sequence-369370-        2772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes HIS3 as a yeast Vector selectable marker to be used in the 
            Ellis MYT system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT031
FEATURES             Location/Qualifiers
     source          1..2772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(184..218)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(289..948)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     863..882
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1130..1718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1619..1638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1879..2066
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2067..2729
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta tgatactgaa
       61 gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg ctttcctttt
      121 ttctttttgc tttttctttt tttttctctt gaactcgacg gatcatagag tatgtcttca
      181 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      241 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
      301 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
      361 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
      421 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
      481 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
      541 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
      601 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
      661 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
      721 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
      781 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
      841 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
      901 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
      961 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     1021 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt
     1081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1141 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1201 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1261 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1321 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1381 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1441 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1501 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1561 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1621 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1681 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1741 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1801 attctgtgga taaccgtgaa gacattacaa acacagtcct ttcccgcaat tttctttttc
     1861 tattactctt ggcctcctct agtacactct atattttttt atgcctcggt aatgattttc
     1921 attttttttt ttccacctag cggatgactc tttttttttc ttagcgattg gcattatcac
     1981 ataatgaatt atacattata taaagtaatg tgatttcttc gaagaatata ctaaaaaatg
     2041 agcaggcaag ataaacgaag gcaaagatga cagagcagaa agccctagta aagcgtatta
     2101 caaatgaaac caagattcag attgcgatct ctttaaaggg tggtccccta gcgatagagc
     2161 actcgatctt cccagaaaaa gaggcagaag cagtagcaga acaggccaca caatcgcaag
     2221 tgattaacgt ccacacaggt atagggtttc tggaccatat gatacatgct ctggccaagc
     2281 attccggctg gtcgctaatc gttgagtgca ttggtgactt acacatagac gaccatcaca
     2341 ccactgagga ctgcgggatt gctctcggtc aagcttttaa agaggcccta ggggccgtgc
     2401 gtggagtaaa aaggtttgga tcaggatttg cgcctttgga tgaggcactt tccagagcgg
     2461 tggttgatct ttcgaacagg ccgtacgcag ttgtcgaact tggtttgcaa agggagaaag
     2521 taggtgatct ctcttgcgag atgatcccgc attttcttga aagctttgca gaggctagca
     2581 gaattaccct ccacgttgat tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg
     2641 cgttcaaggc tcttgcggtt gccataagag aagccacctc gcccaatggt accaacgatg
     2701 ttccctccac caaaggtgtt cttatgtagt gacaccgatt atttaaagct gctgcatacg
     2761 atatatatac at
//
